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Urine accurately reflects circulating isoflavonoids and ascertains compliance during soy intervention.

Abstract

BACKGROUND

Isoflavonoids (IFL) may protect against chronic diseases, including cancer. IFL exposure is traditionally measured from plasma (PL), but the reliability of urine is uncertain. We assessed whether IFL excretion in overnight urine (OU) or spot urine (SU) reliably reflects IFLs in PL and the usefulness of the three matrices to determine soy intake compliance.

METHODS

In a randomized, double-blind, placebo-controlled soy intervention trial with 350 postmenopausal women, IFLs (daidzein, genistein, glycitein, equol, O-desmethylangolensin, dihydrodaidzein, dihydrogenistein) were analyzed by liquid chromatography/mass spectrometry in OU, SU, and PL collected at baseline and every 6 months over 2.5 years.

RESULTS

High between-subject intraclass correlations between all three matrices (median, 0.94) and high between-subject Pearson correlations (median r(OU-PL) = 0.80; median r(SU-PL) = 0.80; median r(OU-SU) = 0.92) allowed the development of equations to predict IFL values from any of the three matrices. Equations developed from a randomly selected 87% of all available data were valid because high correlations were found on the residual 13% of data between equation-generated and measured IFL values (median r(OU-PL) = 0.86; median r(SU-PL) = 0.78; median r(OU-SU) = 0.84); median absolute IFL differences for OU-PL, SU-PL, and OU-SU were 8.8 nmol/L, 10.3 nmol/L, and 0.28 nmol/mg, respectively. All three matrices showed highly significant IFL differences between the placebo and soy intervention group at study end (P < 0.0001) and highly significant correlations between IFL values and counted soy doses in the intervention group.

CONCLUSIONS

OU and SU IFL excretion reflect circulating PL IFL levels in healthy postmenopausal women accurately.

IMPACT

Noninvasively-collected urine can be used to reliably determine systemic IFL exposure and soy intake compliance.

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  • Authors+Show Affiliations

    ,

    Cancer Research Center of Hawaii, 1236 Lauhala Street, Honolulu, HI 96813, USA. adrian@crch.hawaii.edu

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    Source

    MeSH

    Adult
    Aged
    Double-Blind Method
    Female
    Humans
    Isoflavones
    Middle Aged
    Milk Proteins
    Neoplasms
    Patient Compliance
    Placebos
    Postmenopause
    Soybean Proteins

    Pub Type(s)

    Journal Article
    Randomized Controlled Trial
    Research Support, N.I.H., Extramural

    Language

    eng

    PubMed ID

    20615889

    Citation

    Franke, Adrian A., et al. "Urine Accurately Reflects Circulating Isoflavonoids and Ascertains Compliance During Soy Intervention." Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored By the American Society of Preventive Oncology, vol. 19, no. 7, 2010, pp. 1775-83.
    Franke AA, Hebshi SM, Pagano I, et al. Urine accurately reflects circulating isoflavonoids and ascertains compliance during soy intervention. Cancer Epidemiol Biomarkers Prev. 2010;19(7):1775-83.
    Franke, A. A., Hebshi, S. M., Pagano, I., Kono, N., Mack, W. J., & Hodis, H. N. (2010). Urine accurately reflects circulating isoflavonoids and ascertains compliance during soy intervention. Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored By the American Society of Preventive Oncology, 19(7), pp. 1775-83. doi:10.1158/1055-9965.EPI-10-0116.
    Franke AA, et al. Urine Accurately Reflects Circulating Isoflavonoids and Ascertains Compliance During Soy Intervention. Cancer Epidemiol Biomarkers Prev. 2010;19(7):1775-83. PubMed PMID: 20615889.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Urine accurately reflects circulating isoflavonoids and ascertains compliance during soy intervention. AU - Franke,Adrian A, AU - Hebshi,Sandra M, AU - Pagano,Ian, AU - Kono,Naoko, AU - Mack,Wendy J, AU - Hodis,Howard N, PY - 2010/7/10/entrez PY - 2010/7/10/pubmed PY - 2010/10/29/medline SP - 1775 EP - 83 JF - Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology JO - Cancer Epidemiol. Biomarkers Prev. VL - 19 IS - 7 N2 - BACKGROUND: Isoflavonoids (IFL) may protect against chronic diseases, including cancer. IFL exposure is traditionally measured from plasma (PL), but the reliability of urine is uncertain. We assessed whether IFL excretion in overnight urine (OU) or spot urine (SU) reliably reflects IFLs in PL and the usefulness of the three matrices to determine soy intake compliance. METHODS: In a randomized, double-blind, placebo-controlled soy intervention trial with 350 postmenopausal women, IFLs (daidzein, genistein, glycitein, equol, O-desmethylangolensin, dihydrodaidzein, dihydrogenistein) were analyzed by liquid chromatography/mass spectrometry in OU, SU, and PL collected at baseline and every 6 months over 2.5 years. RESULTS: High between-subject intraclass correlations between all three matrices (median, 0.94) and high between-subject Pearson correlations (median r(OU-PL) = 0.80; median r(SU-PL) = 0.80; median r(OU-SU) = 0.92) allowed the development of equations to predict IFL values from any of the three matrices. Equations developed from a randomly selected 87% of all available data were valid because high correlations were found on the residual 13% of data between equation-generated and measured IFL values (median r(OU-PL) = 0.86; median r(SU-PL) = 0.78; median r(OU-SU) = 0.84); median absolute IFL differences for OU-PL, SU-PL, and OU-SU were 8.8 nmol/L, 10.3 nmol/L, and 0.28 nmol/mg, respectively. All three matrices showed highly significant IFL differences between the placebo and soy intervention group at study end (P < 0.0001) and highly significant correlations between IFL values and counted soy doses in the intervention group. CONCLUSIONS: OU and SU IFL excretion reflect circulating PL IFL levels in healthy postmenopausal women accurately. IMPACT: Noninvasively-collected urine can be used to reliably determine systemic IFL exposure and soy intake compliance. SN - 1538-7755 UR - https://www.unboundmedicine.com/medline/citation/20615889/Urine_accurately_reflects_circulating_isoflavonoids_and_ascertains_compliance_during_soy_intervention_ L2 - http://cebp.aacrjournals.org/cgi/pmidlookup?view=long&amp;pmid=20615889 DB - PRIME DP - Unbound Medicine ER -