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Genes encoding the alpha-carboxyltransferase subunit of acetyl-CoA carboxylase from Brassica napus and parental species: cloning, expression patterns, and evolution.
Genome. 2010 May; 53(5):360-70.G

Abstract

Heteromeric acetyl coenzyme A carboxylase (ACCase), a rate-limiting enzyme in fatty acid biosynthesis in dicots, is a multi-enzyme complex consisting of biotin carboxylase, biotin carboxyl carrier protein, and carboxyltransferase (alpha-CT and beta-CT). In the present study, four genes encoding alpha-CT were cloned from Brassica napus, and two were cloned from each of the two parental species, B. rapa and B. oleracea. Comparative and cluster analyses indicated that these genes were divided into two major groups. The major divergence between group-1 and group-2 occurred in the second intron. Group-2 alpha-CT genes represented the ancestral form in the genus Brassica. The divergence of group-1 and group-2 genes occurred in their common ancestor 12.96-17.78 million years ago (MYA), soon after the divergence of Arabidopsis thaliana and Brassica (15-20 MYA). This time of divergence is identical to that reported for the paralogous subgenomes of diploid Brassica species (13-17 MYA). Real-time reverse transcription PCR revealed that the expression patterns of the two groups of genes were similar in different organs, except in leaves. To better understand the regulation and evolution of alpha-CT genes, promoter regions from two sets of orthologous gene copies from B. napus, B. rapa, and B. oleracea were cloned and compared. The function of the promoter of gene Bnalpha-CT-1-1 in group-1 and gene Bnalpha-CT-2-1 in group-2 was examined by assaying beta-glucuronidase activity in transgenic A. thaliana. Our results will be helpful in elucidating the evolution and regulation of ACCase in oilseed rape.

Authors+Show Affiliations

Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20616867

Citation

Li, Zhi-Guo, et al. "Genes Encoding the Alpha-carboxyltransferase Subunit of acetyl-CoA Carboxylase From Brassica Napus and Parental Species: Cloning, Expression Patterns, and Evolution." Genome, vol. 53, no. 5, 2010, pp. 360-70.
Li ZG, Yin WB, Guo H, et al. Genes encoding the alpha-carboxyltransferase subunit of acetyl-CoA carboxylase from Brassica napus and parental species: cloning, expression patterns, and evolution. Genome. 2010;53(5):360-70.
Li, Z. G., Yin, W. B., Guo, H., Song, L. Y., Chen, Y. H., Guan, R. Z., Wang, J. Q., Wang, R. R., & Hu, Z. M. (2010). Genes encoding the alpha-carboxyltransferase subunit of acetyl-CoA carboxylase from Brassica napus and parental species: cloning, expression patterns, and evolution. Genome, 53(5), 360-70. https://doi.org/10.1139/g10-011
Li ZG, et al. Genes Encoding the Alpha-carboxyltransferase Subunit of acetyl-CoA Carboxylase From Brassica Napus and Parental Species: Cloning, Expression Patterns, and Evolution. Genome. 2010;53(5):360-70. PubMed PMID: 20616867.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genes encoding the alpha-carboxyltransferase subunit of acetyl-CoA carboxylase from Brassica napus and parental species: cloning, expression patterns, and evolution. AU - Li,Zhi-Guo, AU - Yin,Wei-Bo, AU - Guo,Huan, AU - Song,Li-Ying, AU - Chen,Yu-Hong, AU - Guan,Rong-Zhan, AU - Wang,Jing-Qiao, AU - Wang,Richard R-C, AU - Hu,Zan-Min, PY - 2010/7/10/entrez PY - 2010/7/10/pubmed PY - 2010/10/30/medline SP - 360 EP - 70 JF - Genome JO - Genome VL - 53 IS - 5 N2 - Heteromeric acetyl coenzyme A carboxylase (ACCase), a rate-limiting enzyme in fatty acid biosynthesis in dicots, is a multi-enzyme complex consisting of biotin carboxylase, biotin carboxyl carrier protein, and carboxyltransferase (alpha-CT and beta-CT). In the present study, four genes encoding alpha-CT were cloned from Brassica napus, and two were cloned from each of the two parental species, B. rapa and B. oleracea. Comparative and cluster analyses indicated that these genes were divided into two major groups. The major divergence between group-1 and group-2 occurred in the second intron. Group-2 alpha-CT genes represented the ancestral form in the genus Brassica. The divergence of group-1 and group-2 genes occurred in their common ancestor 12.96-17.78 million years ago (MYA), soon after the divergence of Arabidopsis thaliana and Brassica (15-20 MYA). This time of divergence is identical to that reported for the paralogous subgenomes of diploid Brassica species (13-17 MYA). Real-time reverse transcription PCR revealed that the expression patterns of the two groups of genes were similar in different organs, except in leaves. To better understand the regulation and evolution of alpha-CT genes, promoter regions from two sets of orthologous gene copies from B. napus, B. rapa, and B. oleracea were cloned and compared. The function of the promoter of gene Bnalpha-CT-1-1 in group-1 and gene Bnalpha-CT-2-1 in group-2 was examined by assaying beta-glucuronidase activity in transgenic A. thaliana. Our results will be helpful in elucidating the evolution and regulation of ACCase in oilseed rape. SN - 1480-3321 UR - https://www.unboundmedicine.com/medline/citation/20616867/Genes_encoding_the_alpha_carboxyltransferase_subunit_of_acetyl_CoA_carboxylase_from_Brassica_napus_and_parental_species:_cloning_expression_patterns_and_evolution_ L2 - https://cdnsciencepub.com/doi/10.1139/g10-011?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -