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On-line coupling of counter-current chromatography and macroporous resin chromatography for continuous isolation of arctiin from the fruit of Arctium lappa L.
J Chromatogr A. 2010 Aug 13; 1217(33):5398-406.JC

Abstract

In this work, we have developed a novel hybrid two-dimensional counter-current chromatography and liquid chromatography (2D CCC x LC) system for the continuous purification of arctiin from crude extract of Arctium lappa. The first dimensional CCC column has been designed to fractionalize crude complex extract into pure arctiin effluent using a one-component organic/salt-containing system, and the second dimensional LC column has been packed with macroporous resin for on-line adsorption, desalination and desorption of arctiin which was effluent purified from the first CCC dimension. Thus, the crude arctiin mixture has been purified efficiently and conveniently by on-line CCC x LC in spite of the use of a salt-containing solvent system in CCC separation. As a result, high purity (more than 97%) of arctiin has been isolated by repeated injections both using the ethyl acetate-8% sodium chloride aqueous solution and butanol-1% sodium chloride aqueous solution. By contrast with the traditional CCC processes using multi-component organic/aqueous solvent systems, the present on-line CCC x LC process only used a one-component organic solvent and thus the solvent is easier to recover and regenerate. All of used solvents such as ethyl acetate, n-butanol and NaCl aqueous solution are low toxicity and environment-friendly. Moreover, the lower phase of salt-containing aqueous solution used as mobile phase, only contained minor organic solvent, which will save much organic solvent in continuous separation. In summary, our results indicated that the on-line hybrid 2D CCC x LC system using one-component organic/salt-containing aqueous solution is very promising and powerful tool for high-throughput purification of arctiin from fruits of A. lappa.

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Authors+Show Affiliations

Guo M
Zhejiang University, College of Life Sciences, Research Center of Siyuan Natural Pharmacy and Biotoxicology, Zijingang Campus, Hangzhou, Zhejiang Province 310058, China.
Liang J
No affiliation info available
Wu S
No affiliation info available

MeSH

AcetatesAdsorptionArctiumButanolsChromatography, High Pressure LiquidCountercurrent DistributionDrugs, Chinese HerbalFruitFuransGlucosidesPorositySodium Chloride

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20619844

Citation

Guo, Mengzhe, et al. "On-line Coupling of Counter-current Chromatography and Macroporous Resin Chromatography for Continuous Isolation of Arctiin From the Fruit of Arctium Lappa L." Journal of Chromatography. A, vol. 1217, no. 33, 2010, pp. 5398-406.
Guo M, Liang J, Wu S. On-line coupling of counter-current chromatography and macroporous resin chromatography for continuous isolation of arctiin from the fruit of Arctium lappa L. J Chromatogr A. 2010;1217(33):5398-406.
Guo, M., Liang, J., & Wu, S. (2010). On-line coupling of counter-current chromatography and macroporous resin chromatography for continuous isolation of arctiin from the fruit of Arctium lappa L. Journal of Chromatography. A, 1217(33), 5398-406. https://doi.org/10.1016/j.chroma.2010.06.038
Guo M, Liang J, Wu S. On-line Coupling of Counter-current Chromatography and Macroporous Resin Chromatography for Continuous Isolation of Arctiin From the Fruit of Arctium Lappa L. J Chromatogr A. 2010 Aug 13;1217(33):5398-406. PubMed PMID: 20619844.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - On-line coupling of counter-current chromatography and macroporous resin chromatography for continuous isolation of arctiin from the fruit of Arctium lappa L. AU - Guo,Mengzhe, AU - Liang,Junling, AU - Wu,Shihua, Y1 - 2010/06/19/ PY - 2010/01/16/received PY - 2010/06/12/revised PY - 2010/06/15/accepted PY - 2010/7/13/entrez PY - 2010/7/14/pubmed PY - 2010/10/29/medline SP - 5398 EP - 406 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1217 IS - 33 N2 - In this work, we have developed a novel hybrid two-dimensional counter-current chromatography and liquid chromatography (2D CCC x LC) system for the continuous purification of arctiin from crude extract of Arctium lappa. The first dimensional CCC column has been designed to fractionalize crude complex extract into pure arctiin effluent using a one-component organic/salt-containing system, and the second dimensional LC column has been packed with macroporous resin for on-line adsorption, desalination and desorption of arctiin which was effluent purified from the first CCC dimension. Thus, the crude arctiin mixture has been purified efficiently and conveniently by on-line CCC x LC in spite of the use of a salt-containing solvent system in CCC separation. As a result, high purity (more than 97%) of arctiin has been isolated by repeated injections both using the ethyl acetate-8% sodium chloride aqueous solution and butanol-1% sodium chloride aqueous solution. By contrast with the traditional CCC processes using multi-component organic/aqueous solvent systems, the present on-line CCC x LC process only used a one-component organic solvent and thus the solvent is easier to recover and regenerate. All of used solvents such as ethyl acetate, n-butanol and NaCl aqueous solution are low toxicity and environment-friendly. Moreover, the lower phase of salt-containing aqueous solution used as mobile phase, only contained minor organic solvent, which will save much organic solvent in continuous separation. In summary, our results indicated that the on-line hybrid 2D CCC x LC system using one-component organic/salt-containing aqueous solution is very promising and powerful tool for high-throughput purification of arctiin from fruits of A. lappa. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/20619844/On_line_coupling_of_counter_current_chromatography_and_macroporous_resin_chromatography_for_continuous_isolation_of_arctiin_from_the_fruit_of_Arctium_lappa_L_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(10)00808-3 DB - PRIME DP - Unbound Medicine ER -