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Hepatitis B virus X protein promotes hypermethylation of p16(INK4A) promoter through upregulation of DNA methyltransferases in hepatocarcinogenesis.
Exp Mol Pathol. 2010 Dec; 89(3):268-75.EM

Abstract

The hepatitis B virus×protein (HBx) has been implicated as a potential trigger of the epigenetic deregulation of some genes, but the underlying mechanism remains unknown. The aim of this study is to identify underlying mechanisms involved in HBx-mediated epigenetic modification in the process of HBx induced p16(INK4A) promoter hypermethylation. Liver cell lines were stably transfected with HBx-expressing vector. The methylation status of p16(INK4A) was examined by methyl-specific polymerase chain reaction (MSP) and bisulfite sequencing. Reverse transcription and real-time polymerase chain reaction (real-time RT-PCR), Western blot and immunohistochemistry were used to analyze the expression of HBx, HBx-mediated DNA methylation abnormalities and p16(INK4A). Some cases of HCC and corresponding noncancerous liver tissues were studied. HBx up-regulates DNMT1 and DNMT3A expression in both mRNA level and protein level, and HBx represses p16(INK4A) expression through inducing hypermethylation of p16(INK4A) promoter. Moreover, HBx induces hypermethylation of p16(INK4A) promoter through DNMT1 and DNMT3A. Regulation of DNMT1 and DNMT3A by HBx promoted hypermethylation of p16(INK4A) promoter region. HBx-DNMTs-p16(INK4A) promoter hypermethylation may suggest a mechanism for tumorigenesis during hepatocarcinogenesis.

Authors+Show Affiliations

Department of Pathology, Shanghai Medical College, Fudan University, Shanghai, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20620135

Citation

Zhu, Ya-Zhen, et al. "Hepatitis B Virus X Protein Promotes Hypermethylation of p16(INK4A) Promoter Through Upregulation of DNA Methyltransferases in Hepatocarcinogenesis." Experimental and Molecular Pathology, vol. 89, no. 3, 2010, pp. 268-75.
Zhu YZ, Zhu R, Shi LG, et al. Hepatitis B virus X protein promotes hypermethylation of p16(INK4A) promoter through upregulation of DNA methyltransferases in hepatocarcinogenesis. Exp Mol Pathol. 2010;89(3):268-75.
Zhu, Y. Z., Zhu, R., Shi, L. G., Mao, Y., Zheng, G. J., Chen, Q., & Zhu, H. G. (2010). Hepatitis B virus X protein promotes hypermethylation of p16(INK4A) promoter through upregulation of DNA methyltransferases in hepatocarcinogenesis. Experimental and Molecular Pathology, 89(3), 268-75. https://doi.org/10.1016/j.yexmp.2010.06.013
Zhu YZ, et al. Hepatitis B Virus X Protein Promotes Hypermethylation of p16(INK4A) Promoter Through Upregulation of DNA Methyltransferases in Hepatocarcinogenesis. Exp Mol Pathol. 2010;89(3):268-75. PubMed PMID: 20620135.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepatitis B virus X protein promotes hypermethylation of p16(INK4A) promoter through upregulation of DNA methyltransferases in hepatocarcinogenesis. AU - Zhu,Ya-Zhen, AU - Zhu,Rong, AU - Shi,Lian-Guo, AU - Mao,Yi, AU - Zheng,Guang-Juan, AU - Chen,Qi, AU - Zhu,Hong-Guang, Y1 - 2010/07/08/ PY - 2010/05/10/received PY - 2010/06/29/accepted PY - 2010/7/13/entrez PY - 2010/7/14/pubmed PY - 2010/12/16/medline SP - 268 EP - 75 JF - Experimental and molecular pathology JO - Exp. Mol. Pathol. VL - 89 IS - 3 N2 - The hepatitis B virus×protein (HBx) has been implicated as a potential trigger of the epigenetic deregulation of some genes, but the underlying mechanism remains unknown. The aim of this study is to identify underlying mechanisms involved in HBx-mediated epigenetic modification in the process of HBx induced p16(INK4A) promoter hypermethylation. Liver cell lines were stably transfected with HBx-expressing vector. The methylation status of p16(INK4A) was examined by methyl-specific polymerase chain reaction (MSP) and bisulfite sequencing. Reverse transcription and real-time polymerase chain reaction (real-time RT-PCR), Western blot and immunohistochemistry were used to analyze the expression of HBx, HBx-mediated DNA methylation abnormalities and p16(INK4A). Some cases of HCC and corresponding noncancerous liver tissues were studied. HBx up-regulates DNMT1 and DNMT3A expression in both mRNA level and protein level, and HBx represses p16(INK4A) expression through inducing hypermethylation of p16(INK4A) promoter. Moreover, HBx induces hypermethylation of p16(INK4A) promoter through DNMT1 and DNMT3A. Regulation of DNMT1 and DNMT3A by HBx promoted hypermethylation of p16(INK4A) promoter region. HBx-DNMTs-p16(INK4A) promoter hypermethylation may suggest a mechanism for tumorigenesis during hepatocarcinogenesis. SN - 1096-0945 UR - https://www.unboundmedicine.com/medline/citation/20620135/Hepatitis_B_virus_X_protein_promotes_hypermethylation_of_p16_INK4A__promoter_through_upregulation_of_DNA_methyltransferases_in_hepatocarcinogenesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4800(10)00097-3 DB - PRIME DP - Unbound Medicine ER -