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Effect of different bile duct flush solutions on biliary tract preservation injury of donated livers for transplantation.

Abstract

OBJECTIVES

The objective of this study was to explore the effect of various bile duct flush (BDF) solutions on biliary tract preservation of donated livers in rats.

METHODS

We studied the effects of BDF solutions and cold ischemic times on biliary tract preservation, using 2 kinds of solutions: (1) BDFa with normal saline (NS), or hypertonic citrate-adenine kidney preservation in vivo (HCA), and (2) BDFb with University of Wisconsin solution (UW), or histidine-tryptophan-ketoglutarate solution (HTK). The cold ischemic times (CIT) were 4, 8, or 12 hours. Forty-five healthy male Wistar rats were randomly divided into 9 groups of 5 rats each using a [L(9)(3(4))] orthogonal table. Biliary tract tissues were examined at the corresponding cold preservation times for the following: microscopic changes in bile duct cells; TUNEL (Transferase-mediated, dUTP-bitin nick end labeling) procedure assays apoptotic indices (AI) of endothelial cells in the biliary tract; ultrastructural changes; and average volumes (V) and density (Nd) of mitochondria in endothelial cells calculated using an image analysis system. The results were evaluated by analysis of variance (ANOVA) and direct analysis by an orthogonal design.

RESULTS

AI of biliary tract endothelial cells showed significance (P < .01) of cold preservation of the biliary tract of donor liver with BDFa or BDFb and CIT; furthermore, HCA, HTK, and 4-hour CIT were all ideal. V and Nd of mitochondrial endothelial cells were significantly increased (P < .01) with BDFa, BDFb, and CIT; furthermore, factors HCA, HTK, and 4-hour CIT were all ideal.

CONCLUSIONS

Cold preservation injuries to the biliary tract of a donor liver may be greatly decreased by efficient and sufficient flushing of the bile tract. A suitable bile duct solution greatly decreases cold preservation injuries and protects endothelial cells.

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  • Authors+Show Affiliations

    ,

    Department of Hepatopancreatobiliary Surgery, Shengjing Hospital affiliated to China Medical University, Shenyang City, China. cuidx@sj-hospital.org

    , , , ,

    Source

    Transplantation proceedings 42:5 2010 Jun pg 1576-81

    MeSH

    Animals
    Apoptosis
    Bile
    Bile Ducts
    Biliary Tract
    Epithelial Cells
    Humans
    In Situ Nick-End Labeling
    Liver
    Liver Transplantation
    Male
    Organ Preservation
    Organ Preservation Solutions
    Rats
    Tissue Donors

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    20620477

    Citation

    Cui, D X., et al. "Effect of Different Bile Duct Flush Solutions On Biliary Tract Preservation Injury of Donated Livers for Transplantation." Transplantation Proceedings, vol. 42, no. 5, 2010, pp. 1576-81.
    Cui DX, Yin JQ, Xu WX, et al. Effect of different bile duct flush solutions on biliary tract preservation injury of donated livers for transplantation. Transplant Proc. 2010;42(5):1576-81.
    Cui, D. X., Yin, J. Q., Xu, W. X., Chai, F., Liu, B. L., & Zhang, X. B. (2010). Effect of different bile duct flush solutions on biliary tract preservation injury of donated livers for transplantation. Transplantation Proceedings, 42(5), pp. 1576-81. doi:10.1016/j.transproceed.2009.12.057.
    Cui DX, et al. Effect of Different Bile Duct Flush Solutions On Biliary Tract Preservation Injury of Donated Livers for Transplantation. Transplant Proc. 2010;42(5):1576-81. PubMed PMID: 20620477.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Effect of different bile duct flush solutions on biliary tract preservation injury of donated livers for transplantation. AU - Cui,D X, AU - Yin,J Q, AU - Xu,W X, AU - Chai,F, AU - Liu,B L, AU - Zhang,X B, PY - 2009/02/16/received PY - 2009/12/29/accepted PY - 2010/7/13/entrez PY - 2010/7/14/pubmed PY - 2010/10/30/medline SP - 1576 EP - 81 JF - Transplantation proceedings JO - Transplant. Proc. VL - 42 IS - 5 N2 - OBJECTIVES: The objective of this study was to explore the effect of various bile duct flush (BDF) solutions on biliary tract preservation of donated livers in rats. METHODS: We studied the effects of BDF solutions and cold ischemic times on biliary tract preservation, using 2 kinds of solutions: (1) BDFa with normal saline (NS), or hypertonic citrate-adenine kidney preservation in vivo (HCA), and (2) BDFb with University of Wisconsin solution (UW), or histidine-tryptophan-ketoglutarate solution (HTK). The cold ischemic times (CIT) were 4, 8, or 12 hours. Forty-five healthy male Wistar rats were randomly divided into 9 groups of 5 rats each using a [L(9)(3(4))] orthogonal table. Biliary tract tissues were examined at the corresponding cold preservation times for the following: microscopic changes in bile duct cells; TUNEL (Transferase-mediated, dUTP-bitin nick end labeling) procedure assays apoptotic indices (AI) of endothelial cells in the biliary tract; ultrastructural changes; and average volumes (V) and density (Nd) of mitochondria in endothelial cells calculated using an image analysis system. The results were evaluated by analysis of variance (ANOVA) and direct analysis by an orthogonal design. RESULTS: AI of biliary tract endothelial cells showed significance (P < .01) of cold preservation of the biliary tract of donor liver with BDFa or BDFb and CIT; furthermore, HCA, HTK, and 4-hour CIT were all ideal. V and Nd of mitochondrial endothelial cells were significantly increased (P < .01) with BDFa, BDFb, and CIT; furthermore, factors HCA, HTK, and 4-hour CIT were all ideal. CONCLUSIONS: Cold preservation injuries to the biliary tract of a donor liver may be greatly decreased by efficient and sufficient flushing of the bile tract. A suitable bile duct solution greatly decreases cold preservation injuries and protects endothelial cells. SN - 1873-2623 UR - https://www.unboundmedicine.com/medline/citation/20620477/Effect_of_different_bile_duct_flush_solutions_on_biliary_tract_preservation_injury_of_donated_livers_for_transplantation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-1345(10)00497-5 DB - PRIME DP - Unbound Medicine ER -