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Polymorphisms in PPARD, PPARG and APM1 associated with four types of traditional Chinese medicine constitutions.
J Genet Genomics. 2010 Jun; 37(6):371-9.JG

Abstract

Based on the theory of constitution of Traditional Chinese Medicine (TCM), the human population is divided into nine constitutions including one balanced constitution (Normality) and eight unbalanced constitutions (Yang-deficiency, Yin-deficiency, Phlegm-wetness, Qi-deficiency, Wetness-heat, Blood stasis, Depressed constitution, and Inherited special constitution). Different constitutions have specific metabolic features and different susceptibility to certain diseases. However, whether a genetic basis accounts for such constitution classification is yet to be determined. Here we performed a genetic study to assess the association between genetic variations of metabolic genes including PPARD, PPARG and APM1 and the constitutions. A total of 233 individuals of the Han population in China were classified into four groups, Normality, Yang-deficiency, Yin-deficiency and Phlegm-wetness with whom 23 single nucleotide polymorphisms (SNPs) in the three genes were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Biased distribution of PPARD rs2267669 and rs2076167, APM1 rs7627128 and rs1063539 in Yang-deficiency, PPARG Pro12Ala in Yin-deficiency and PPARD rs2076167, APM1 rs266729 and rs7627128 in Phlegm-wetness were observed. The frequencies of Haplotype13 (Hap13) of PPARG in Yin-deficiency, Hap25 of APM1 in Yang-deficiency and Hap2 of PPARD and Hap14 of PPARG in Phlegm-wetness, were significantly different from those in Normality, suggesting those might be group-associated haplotypes. These results suggested that single SNP and haplotypes of PPARD, PPARG and APM1 may underlie the genetic basis of the constitutions classified in TCM.

Authors+Show Affiliations

Human Genetics Center of Yunnan University, Kunming, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20621019

Citation

Wu, Yanrui, et al. "Polymorphisms in PPARD, PPARG and APM1 Associated With Four Types of Traditional Chinese Medicine Constitutions." Journal of Genetics and Genomics = Yi Chuan Xue Bao, vol. 37, no. 6, 2010, pp. 371-9.
Wu Y, Cun Y, Dong J, et al. Polymorphisms in PPARD, PPARG and APM1 associated with four types of traditional Chinese medicine constitutions. J Genet Genomics. 2010;37(6):371-9.
Wu, Y., Cun, Y., Dong, J., Shao, J., Luo, S., Nie, S., Yu, H., Zheng, B., Wang, Q., & Xiao, C. (2010). Polymorphisms in PPARD, PPARG and APM1 associated with four types of traditional Chinese medicine constitutions. Journal of Genetics and Genomics = Yi Chuan Xue Bao, 37(6), 371-9. https://doi.org/10.1016/S1673-8527(09)60055-2
Wu Y, et al. Polymorphisms in PPARD, PPARG and APM1 Associated With Four Types of Traditional Chinese Medicine Constitutions. J Genet Genomics. 2010;37(6):371-9. PubMed PMID: 20621019.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polymorphisms in PPARD, PPARG and APM1 associated with four types of traditional Chinese medicine constitutions. AU - Wu,Yanrui, AU - Cun,Yina, AU - Dong,Jing, AU - Shao,Jingru, AU - Luo,Shengjun, AU - Nie,Shengjie, AU - Yu,Haijing, AU - Zheng,Bingrong, AU - Wang,Qi, AU - Xiao,Chunjie, Y1 - 2010/07/01/ PY - 2009/12/10/received PY - 2010/04/30/revised PY - 2010/05/10/accepted PY - 2010/7/13/entrez PY - 2010/7/14/pubmed PY - 2010/10/29/medline SP - 371 EP - 9 JF - Journal of genetics and genomics = Yi chuan xue bao JO - J Genet Genomics VL - 37 IS - 6 N2 - Based on the theory of constitution of Traditional Chinese Medicine (TCM), the human population is divided into nine constitutions including one balanced constitution (Normality) and eight unbalanced constitutions (Yang-deficiency, Yin-deficiency, Phlegm-wetness, Qi-deficiency, Wetness-heat, Blood stasis, Depressed constitution, and Inherited special constitution). Different constitutions have specific metabolic features and different susceptibility to certain diseases. However, whether a genetic basis accounts for such constitution classification is yet to be determined. Here we performed a genetic study to assess the association between genetic variations of metabolic genes including PPARD, PPARG and APM1 and the constitutions. A total of 233 individuals of the Han population in China were classified into four groups, Normality, Yang-deficiency, Yin-deficiency and Phlegm-wetness with whom 23 single nucleotide polymorphisms (SNPs) in the three genes were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Biased distribution of PPARD rs2267669 and rs2076167, APM1 rs7627128 and rs1063539 in Yang-deficiency, PPARG Pro12Ala in Yin-deficiency and PPARD rs2076167, APM1 rs266729 and rs7627128 in Phlegm-wetness were observed. The frequencies of Haplotype13 (Hap13) of PPARG in Yin-deficiency, Hap25 of APM1 in Yang-deficiency and Hap2 of PPARD and Hap14 of PPARG in Phlegm-wetness, were significantly different from those in Normality, suggesting those might be group-associated haplotypes. These results suggested that single SNP and haplotypes of PPARD, PPARG and APM1 may underlie the genetic basis of the constitutions classified in TCM. SN - 1673-8527 UR - https://www.unboundmedicine.com/medline/citation/20621019/Polymorphisms_in_PPARD_PPARG_and_APM1_associated_with_four_types_of_traditional_Chinese_medicine_constitutions_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1673-8527(09)60055-2 DB - PRIME DP - Unbound Medicine ER -