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Bioassay-guided isolation of anti-inflammatory, antinociceptive and wound healer glycosides from the flowers of Verbascum mucronatum Lam.
J Ethnopharmacol. 2011 Jul 14; 136(3):436-43.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

The leaves, flowers and whole aerial parts of Verbascum L. species have been used to treat respiratory problems, haemorrhoids and other types of inflammatory conditions in traditional Turkish medicine.

AIM OF THE STUDY

In order to evaluate this traditional information, the anti-inflammatory, antinociceptive and wound healing activities of Verbascum mucronatum Lam. which is used as haemostatic in Turkish folk medicine were investigated.

MATERIALS AND METHODS

In vivo inhibitory effect of the extracts on the carrageenan-induced hind paw edema model in mice was studied for the assessment of anti-inflammatory activity. Moreover, the wound healing potential of the plant were evaluated by using in vivo wound healing experimental models, i.e. incision and excision models on mice and rats, were comparatively assessed with a reference ointment Madecassol(®). Skin samples were also evaluated histopathologically.

RESULTS

The results of these experimental studies exhibited that Verbascum mucronatum displays anti-inflammatory, antinociceptive and wound healing activities. Through bioassay-guided fractionation and isolation procedures four iridoid glucosides, ajugol (1), aucubin (2), lasianthoside I (3), catalpol (4), two saponins, ilwensisaponin A (5) and C (6) and a phenylethanoid glycoside, verbascoside (7) were isolated and their structures were elucidated by spectral techniques. Verbascoside (7) was found to possess significant wound healing activity as well as antinociceptive and anti-inflammatory potentials, per os without inducing any apparent acute toxicity or gastric damage.

CONCLUSION

The experimental study revealed that Verbascum mucronatum displays remarkable antinociceptive, anti-inflammatory and wound healing activities.

Authors+Show Affiliations

Department of Pharmacognosy, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20621642

Citation

Akdemir, Zeliha, et al. "Bioassay-guided Isolation of Anti-inflammatory, Antinociceptive and Wound Healer Glycosides From the Flowers of Verbascum Mucronatum Lam." Journal of Ethnopharmacology, vol. 136, no. 3, 2011, pp. 436-43.
Akdemir Z, Kahraman C, Tatlı II, et al. Bioassay-guided isolation of anti-inflammatory, antinociceptive and wound healer glycosides from the flowers of Verbascum mucronatum Lam. J Ethnopharmacol. 2011;136(3):436-43.
Akdemir, Z., Kahraman, C., Tatlı, I. I., Küpeli Akkol, E., Süntar, I., & Keles, H. (2011). Bioassay-guided isolation of anti-inflammatory, antinociceptive and wound healer glycosides from the flowers of Verbascum mucronatum Lam. Journal of Ethnopharmacology, 136(3), 436-43. https://doi.org/10.1016/j.jep.2010.05.059
Akdemir Z, et al. Bioassay-guided Isolation of Anti-inflammatory, Antinociceptive and Wound Healer Glycosides From the Flowers of Verbascum Mucronatum Lam. J Ethnopharmacol. 2011 Jul 14;136(3):436-43. PubMed PMID: 20621642.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bioassay-guided isolation of anti-inflammatory, antinociceptive and wound healer glycosides from the flowers of Verbascum mucronatum Lam. AU - Akdemir,Zeliha, AU - Kahraman,Ciğdem, AU - Tatlı,I Irem, AU - Küpeli Akkol,Esra, AU - Süntar,Ipek, AU - Keles,Hikmet, Y1 - 2010/06/04/ PY - 2010/02/09/received PY - 2010/05/26/revised PY - 2010/05/28/accepted PY - 2010/7/13/entrez PY - 2010/7/14/pubmed PY - 2012/1/11/medline SP - 436 EP - 43 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 136 IS - 3 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: The leaves, flowers and whole aerial parts of Verbascum L. species have been used to treat respiratory problems, haemorrhoids and other types of inflammatory conditions in traditional Turkish medicine. AIM OF THE STUDY: In order to evaluate this traditional information, the anti-inflammatory, antinociceptive and wound healing activities of Verbascum mucronatum Lam. which is used as haemostatic in Turkish folk medicine were investigated. MATERIALS AND METHODS: In vivo inhibitory effect of the extracts on the carrageenan-induced hind paw edema model in mice was studied for the assessment of anti-inflammatory activity. Moreover, the wound healing potential of the plant were evaluated by using in vivo wound healing experimental models, i.e. incision and excision models on mice and rats, were comparatively assessed with a reference ointment Madecassol(®). Skin samples were also evaluated histopathologically. RESULTS: The results of these experimental studies exhibited that Verbascum mucronatum displays anti-inflammatory, antinociceptive and wound healing activities. Through bioassay-guided fractionation and isolation procedures four iridoid glucosides, ajugol (1), aucubin (2), lasianthoside I (3), catalpol (4), two saponins, ilwensisaponin A (5) and C (6) and a phenylethanoid glycoside, verbascoside (7) were isolated and their structures were elucidated by spectral techniques. Verbascoside (7) was found to possess significant wound healing activity as well as antinociceptive and anti-inflammatory potentials, per os without inducing any apparent acute toxicity or gastric damage. CONCLUSION: The experimental study revealed that Verbascum mucronatum displays remarkable antinociceptive, anti-inflammatory and wound healing activities. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/20621642/Bioassay_guided_isolation_of_anti_inflammatory_antinociceptive_and_wound_healer_glycosides_from_the_flowers_of_Verbascum_mucronatum_Lam_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(10)00379-X DB - PRIME DP - Unbound Medicine ER -