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RETRACTED ARTICLE

Quantifying conversion of linoleic to arachidonic and other n-6 polyunsaturated fatty acids in unanesthetized rats.
J Lipid Res. 2010 Oct; 51(10):2940-6.JL

Abstract

Isotope feeding studies report a wide range of conversion fractions of dietary shorter-chain polyunsaturated fatty acids (PUFAs) to long-chain PUFAs, which limits assessing nutritional requirements and organ effects of arachidonic (AA, 20:4n-6) and docosahexaenoic (DHA, 22:6n-3) acids. In this study, whole-body (largely liver) steady-state conversion coefficients and rates of circulating unesterified linoleic acid (LA, 18:2n-6) to esterified AA and other elongated n-6 PUFAs were quantified directly using operational equations, in unanesthetized adult rats on a high-DHA but AA-free diet, using 2 h of intravenous [U-(13)C]LA infusion. Unesterified LA was converted to esterified LA in plasma at a greater rate than to esterified gamma-linolenic (gamma-LNA, 18:3n-6), eicosatrienoic acid (ETA, 20:3n-6), or AA. The steady-state whole-body synthesis-secretion (conversion) coefficient k*(i) to AA equaled 5.4 x 10(-3) min(-1), while the conversion rate (coefficient x concentration) equaled 16.1 micromol/day. This rate exceeds the reported brain AA consumption rate by 27-fold. As brain and heart cannot synthesize significant AA from circulating LA, liver synthesis is necessary to maintain their homeostatic AA concentrations in the absence of dietary AA. The heavy-isotope intravenous infusion method could be used to quantify steady-state liver synthesis-secretion of AA from LA under different conditions in rodents and in humans.

Authors+Show Affiliations

Brain Physiology and Metabolism Section, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA. gaof@mail.nih.govNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Intramural
Retracted Publication

Language

eng

PubMed ID

20622136

Citation

Gao, Fei, et al. "Quantifying Conversion of Linoleic to Arachidonic and Other N-6 Polyunsaturated Fatty Acids in Unanesthetized Rats." Journal of Lipid Research, vol. 51, no. 10, 2010, pp. 2940-6.
Gao F, Kiesewetter D, Chang L, et al. Quantifying conversion of linoleic to arachidonic and other n-6 polyunsaturated fatty acids in unanesthetized rats. J Lipid Res. 2010;51(10):2940-6.
Gao, F., Kiesewetter, D., Chang, L., Rapoport, S. I., & Igarashi, M. (2010). Quantifying conversion of linoleic to arachidonic and other n-6 polyunsaturated fatty acids in unanesthetized rats. Journal of Lipid Research, 51(10), 2940-6. https://doi.org/10.1194/jlr.M005595
Gao F, et al. Quantifying Conversion of Linoleic to Arachidonic and Other N-6 Polyunsaturated Fatty Acids in Unanesthetized Rats. J Lipid Res. 2010;51(10):2940-6. PubMed PMID: 20622136.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantifying conversion of linoleic to arachidonic and other n-6 polyunsaturated fatty acids in unanesthetized rats. AU - Gao,Fei, AU - Kiesewetter,Dale, AU - Chang,Lisa, AU - Rapoport,Stanley I, AU - Igarashi,Miki, Y1 - 2010/07/09/ PY - 2010/7/13/entrez PY - 2010/7/14/pubmed PY - 2011/1/25/medline SP - 2940 EP - 6 JF - Journal of lipid research JO - J Lipid Res VL - 51 IS - 10 N2 - Isotope feeding studies report a wide range of conversion fractions of dietary shorter-chain polyunsaturated fatty acids (PUFAs) to long-chain PUFAs, which limits assessing nutritional requirements and organ effects of arachidonic (AA, 20:4n-6) and docosahexaenoic (DHA, 22:6n-3) acids. In this study, whole-body (largely liver) steady-state conversion coefficients and rates of circulating unesterified linoleic acid (LA, 18:2n-6) to esterified AA and other elongated n-6 PUFAs were quantified directly using operational equations, in unanesthetized adult rats on a high-DHA but AA-free diet, using 2 h of intravenous [U-(13)C]LA infusion. Unesterified LA was converted to esterified LA in plasma at a greater rate than to esterified gamma-linolenic (gamma-LNA, 18:3n-6), eicosatrienoic acid (ETA, 20:3n-6), or AA. The steady-state whole-body synthesis-secretion (conversion) coefficient k*(i) to AA equaled 5.4 x 10(-3) min(-1), while the conversion rate (coefficient x concentration) equaled 16.1 micromol/day. This rate exceeds the reported brain AA consumption rate by 27-fold. As brain and heart cannot synthesize significant AA from circulating LA, liver synthesis is necessary to maintain their homeostatic AA concentrations in the absence of dietary AA. The heavy-isotope intravenous infusion method could be used to quantify steady-state liver synthesis-secretion of AA from LA under different conditions in rodents and in humans. SN - 1539-7262 UR - https://www.unboundmedicine.com/medline/citation/20622136/Quantifying_conversion_of_linoleic_to_arachidonic_and_other_n_6_polyunsaturated_fatty_acids_in_unanesthetized_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2275(20)40983-6 DB - PRIME DP - Unbound Medicine ER -