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The nitrative and oxidative stress in blood platelets isolated from breast cancer patients: the protectory action of aronia melanocarpa extract.
Platelets 2010; 21(7):541-8P

Abstract

Since mechanisms involved in the relationship between oxidative stress and breast cancer are still unclear, the aim of our present study was to evaluate oxidative/nitrative modifications of blood platelet proteins by measuring the level of biomarkers of oxidative/nitrative stress such as carbonyl groups, thiol groups and 3-nitrotyrosine in proteins in patients with benign breast diseases and in patients with invasive breast cancer, and compare with the control group. Levels of carbonyl groups and 3-nitrotyrosine residues in platelet proteins were measured by ELISA and a competition ELISA, respectively. The method with 5,5′-dithio-bis(2-nitro-benzoic acid) has been used to analyse free thiol groups in platelet proteins. Patients were hospitalized in the Department of Oncological Surgery, Medical University of Lodz, Poland. Exogenous antioxidants reduce oxidative stress, therefore we also investigated in a model system in vitro the effects of a polyphenol rich extract of Aronia melanocarpa (Rosaceae, final concentration of 50 µg/ml, 5 min, 37°C) on modified blood platelet proteins as well from patients with breast cancer and from the healthy group. We demonstrated in platelet proteins from patients with invasive breast cancer a higher level of carbonyl groups than in the control healthy group (p < 0.02). The level of 3-nitrotyrosine in platelet proteins from patients with invasive breast cancer was also significantly higher than in the healthy subject group (p < 0.001). In contrast, the amount of thiol groups in platelet proteins from patients was significantly lower (about < 50%) than in control blood platelets. In a model system in vitro we also observed that the extract from berries of A. melanocarpa (50 µg/ml, 5 min, 37°C) due to antioxidant action, significantly reduced the oxidative/nitrative stress (measured by thiol groups and 3-nitrotyrosine) in platelets, not only from the healthy group, but also from patients with benign breast diseases and in patients with invasive breast cancer.

Authors+Show Affiliations

Department of General Biochemistry, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20624007

Citation

Kedzierska, Magdalena, et al. "The Nitrative and Oxidative Stress in Blood Platelets Isolated From Breast Cancer Patients: the Protectory Action of Aronia Melanocarpa Extract." Platelets, vol. 21, no. 7, 2010, pp. 541-8.
Kedzierska M, Olas B, Wachowicz B, et al. The nitrative and oxidative stress in blood platelets isolated from breast cancer patients: the protectory action of aronia melanocarpa extract. Platelets. 2010;21(7):541-8.
Kedzierska, M., Olas, B., Wachowicz, B., Stochmal, A., Oleszek, W., Jeziorski, A., & Piekarski, J. (2010). The nitrative and oxidative stress in blood platelets isolated from breast cancer patients: the protectory action of aronia melanocarpa extract. Platelets, 21(7), pp. 541-8. doi:10.3109/09537104.2010.492534.
Kedzierska M, et al. The Nitrative and Oxidative Stress in Blood Platelets Isolated From Breast Cancer Patients: the Protectory Action of Aronia Melanocarpa Extract. Platelets. 2010;21(7):541-8. PubMed PMID: 20624007.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The nitrative and oxidative stress in blood platelets isolated from breast cancer patients: the protectory action of aronia melanocarpa extract. AU - Kedzierska,Magdalena, AU - Olas,Beata, AU - Wachowicz,Barbara, AU - Stochmal,Anna, AU - Oleszek,Wieslaw, AU - Jeziorski,Arkadiusz, AU - Piekarski,Janusz, PY - 2010/7/14/entrez PY - 2010/7/14/pubmed PY - 2011/2/26/medline SP - 541 EP - 8 JF - Platelets JO - Platelets VL - 21 IS - 7 N2 - Since mechanisms involved in the relationship between oxidative stress and breast cancer are still unclear, the aim of our present study was to evaluate oxidative/nitrative modifications of blood platelet proteins by measuring the level of biomarkers of oxidative/nitrative stress such as carbonyl groups, thiol groups and 3-nitrotyrosine in proteins in patients with benign breast diseases and in patients with invasive breast cancer, and compare with the control group. Levels of carbonyl groups and 3-nitrotyrosine residues in platelet proteins were measured by ELISA and a competition ELISA, respectively. The method with 5,5′-dithio-bis(2-nitro-benzoic acid) has been used to analyse free thiol groups in platelet proteins. Patients were hospitalized in the Department of Oncological Surgery, Medical University of Lodz, Poland. Exogenous antioxidants reduce oxidative stress, therefore we also investigated in a model system in vitro the effects of a polyphenol rich extract of Aronia melanocarpa (Rosaceae, final concentration of 50 µg/ml, 5 min, 37°C) on modified blood platelet proteins as well from patients with breast cancer and from the healthy group. We demonstrated in platelet proteins from patients with invasive breast cancer a higher level of carbonyl groups than in the control healthy group (p < 0.02). The level of 3-nitrotyrosine in platelet proteins from patients with invasive breast cancer was also significantly higher than in the healthy subject group (p < 0.001). In contrast, the amount of thiol groups in platelet proteins from patients was significantly lower (about < 50%) than in control blood platelets. In a model system in vitro we also observed that the extract from berries of A. melanocarpa (50 µg/ml, 5 min, 37°C) due to antioxidant action, significantly reduced the oxidative/nitrative stress (measured by thiol groups and 3-nitrotyrosine) in platelets, not only from the healthy group, but also from patients with benign breast diseases and in patients with invasive breast cancer. SN - 1369-1635 UR - https://www.unboundmedicine.com/medline/citation/20624007/The_nitrative_and_oxidative_stress_in_blood_platelets_isolated_from_breast_cancer_patients:_the_protectory_action_of_aronia_melanocarpa_extract_ L2 - http://www.tandfonline.com/doi/full/10.3109/09537104.2010.492534 DB - PRIME DP - Unbound Medicine ER -