Tags

Type your tag names separated by a space and hit enter

An H5N1 M2e-based multiple antigenic peptide vaccine confers heterosubtypic protection from lethal infection with pandemic 2009 H1N1 virus.
Virol J. 2010 Jul 12; 7:151.VJ

Abstract

BACKGROUND

A 2009 global influenza pandemic caused by a novel swine-origin H1N1 influenza A virus has posted an increasing threat of a potential pandemic by the highly pathogenic avian influenza (HPAI) H5N1 virus, driving us to develop an influenza vaccine which confers cross-protection against both H5N1 and H1N1 viruses. Previously, we have shown that a tetra-branched multiple antigenic peptide (MAP) vaccine based on the extracellular domain of M2 protein (M2e) from H5N1 virus (H5N1-M2e-MAP) induced strong immune responses and cross-protection against different clades of HPAI H5N1 viruses. In this report, we investigated whether such M2e-MAP presenting the H5N1-M2e consensus sequence can afford heterosubtypic protection from lethal challenge with the pandemic 2009 H1N1 virus.

RESULTS

Our results demonstrated that H5N1-M2e-MAP plus Freund's or aluminum adjuvant induced strong cross-reactive IgG antibody responses against M2e of the pandemic H1N1 virus which contains one amino acid variation with M2e of H5N1 at position 13. These cross-reactive antibodies may maintain for 6 months and bounced back quickly to the previous high level after the 2nd boost administered 2 weeks before virus challenge. H5N1-M2e-MAP could afford heterosubtypic protection against lethal challenge with pandemic H1N1 virus, showing significant decrease of viral replications and obvious alleviation of histopathological damages in the challenged mouse lungs. 100% and 80% of the H5N1-M2e-MAP-vaccinated mice with Freund's and aluminum adjuvant, respectively, survived the lethal challenge with pandemic H1N1 virus.

CONCLUSIONS

Our results suggest that H5N1-M2e-MAP has a great potential to prevent the threat from re-emergence of pandemic H1N1 influenza and possible novel influenza pandemic due to the reassortment of HPAI H5N1 virus with the 2009 swine-origin H1N1 influenza virus.

Authors+Show Affiliations

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20624292

Citation

Zhao, Guangyu, et al. "An H5N1 M2e-based Multiple Antigenic Peptide Vaccine Confers Heterosubtypic Protection From Lethal Infection With Pandemic 2009 H1N1 Virus." Virology Journal, vol. 7, 2010, p. 151.
Zhao G, Sun S, Du L, et al. An H5N1 M2e-based multiple antigenic peptide vaccine confers heterosubtypic protection from lethal infection with pandemic 2009 H1N1 virus. Virol J. 2010;7:151.
Zhao, G., Sun, S., Du, L., Xiao, W., Ru, Z., Kou, Z., Guo, Y., Yu, H., Jiang, S., Lone, Y., Zheng, B. J., & Zhou, Y. (2010). An H5N1 M2e-based multiple antigenic peptide vaccine confers heterosubtypic protection from lethal infection with pandemic 2009 H1N1 virus. Virology Journal, 7, 151. https://doi.org/10.1186/1743-422X-7-151
Zhao G, et al. An H5N1 M2e-based Multiple Antigenic Peptide Vaccine Confers Heterosubtypic Protection From Lethal Infection With Pandemic 2009 H1N1 Virus. Virol J. 2010 Jul 12;7:151. PubMed PMID: 20624292.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An H5N1 M2e-based multiple antigenic peptide vaccine confers heterosubtypic protection from lethal infection with pandemic 2009 H1N1 virus. AU - Zhao,Guangyu, AU - Sun,Shihui, AU - Du,Lanying, AU - Xiao,Wenjun, AU - Ru,Zhitao, AU - Kou,Zhihua, AU - Guo,Yan, AU - Yu,Hong, AU - Jiang,Shibo, AU - Lone,Yuchun, AU - Zheng,Bo-Jian, AU - Zhou,Yusen, Y1 - 2010/07/12/ PY - 2010/05/31/received PY - 2010/07/12/accepted PY - 2010/7/14/entrez PY - 2010/7/14/pubmed PY - 2010/9/8/medline SP - 151 EP - 151 JF - Virology journal JO - Virol J VL - 7 N2 - BACKGROUND: A 2009 global influenza pandemic caused by a novel swine-origin H1N1 influenza A virus has posted an increasing threat of a potential pandemic by the highly pathogenic avian influenza (HPAI) H5N1 virus, driving us to develop an influenza vaccine which confers cross-protection against both H5N1 and H1N1 viruses. Previously, we have shown that a tetra-branched multiple antigenic peptide (MAP) vaccine based on the extracellular domain of M2 protein (M2e) from H5N1 virus (H5N1-M2e-MAP) induced strong immune responses and cross-protection against different clades of HPAI H5N1 viruses. In this report, we investigated whether such M2e-MAP presenting the H5N1-M2e consensus sequence can afford heterosubtypic protection from lethal challenge with the pandemic 2009 H1N1 virus. RESULTS: Our results demonstrated that H5N1-M2e-MAP plus Freund's or aluminum adjuvant induced strong cross-reactive IgG antibody responses against M2e of the pandemic H1N1 virus which contains one amino acid variation with M2e of H5N1 at position 13. These cross-reactive antibodies may maintain for 6 months and bounced back quickly to the previous high level after the 2nd boost administered 2 weeks before virus challenge. H5N1-M2e-MAP could afford heterosubtypic protection against lethal challenge with pandemic H1N1 virus, showing significant decrease of viral replications and obvious alleviation of histopathological damages in the challenged mouse lungs. 100% and 80% of the H5N1-M2e-MAP-vaccinated mice with Freund's and aluminum adjuvant, respectively, survived the lethal challenge with pandemic H1N1 virus. CONCLUSIONS: Our results suggest that H5N1-M2e-MAP has a great potential to prevent the threat from re-emergence of pandemic H1N1 influenza and possible novel influenza pandemic due to the reassortment of HPAI H5N1 virus with the 2009 swine-origin H1N1 influenza virus. SN - 1743-422X UR - https://www.unboundmedicine.com/medline/citation/20624292/An_H5N1_M2e_based_multiple_antigenic_peptide_vaccine_confers_heterosubtypic_protection_from_lethal_infection_with_pandemic_2009_H1N1_virus_ L2 - https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-7-151 DB - PRIME DP - Unbound Medicine ER -