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Thymic stromal lymphopoietin-activated invariant natural killer T cells trigger an innate allergic immune response in atopic dermatitis.
J Allergy Clin Immunol. 2010 Aug; 126(2):290-9, 299.e1-4.JA

Abstract

BACKGROUND

Although invariant natural killer T (iNKT) cells have been shown to play a critical role in the pathogenesis of asthma, the role of iNKT cells in atopic dermatitis (AD) has not been well evaluated.

OBJECTIVE

We investigated whether iNKT cells in patients with AD increased and whether iNKT cells were activated by thymic stromal lymphopoietin (TSLP), which is highly expressed in keratinocytes of AD.

METHODS

We assessed the population of iNKT cells in PBMCs of patients with AD and healthy controls (HCs) using flow cytometry. Immunohistochemistry was used to evaluate iNKT cells and TSLP expression in AD and HC skin. We also evaluated whether iNKT cells expressed the TSLP receptor, the effects of TSLP on iNKT cells, and iNKT cell-dendritic cell interactions in a TSLP-rich environment.

RESULTS

There were more iNKT cells among PBMCs of patients with moderate to severe AD than mild AD (P < .05) and HC (P < .001). The number of iNKT cells was significantly larger in severe AD skin lesions than in mild (P < .001) or moderate AD skin lesions (P < .05). TSLP expression increased in lesional skin (P < .001) but not in the sera of patients with AD (P = .729) compared with HC. iNKT cells expressed TSLP receptor protein and mRNA. TSLP directly activated iNKT cells to secrete IL-4 and IL-13, and the concurrent addition of dendritic cells further activated IFN-gamma expression.

CONCLUSION

Increased iNKT cells activated by TSLP, especially in patients with severe AD, might play an essential role in the innate allergic immune response in AD.

Authors+Show Affiliations

Department of Dermatology and Cutaneous Biology Research Institute, Yonsei University College of Medicine, Seoul, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20624642

Citation

Wu, Wen Hao, et al. "Thymic Stromal Lymphopoietin-activated Invariant Natural Killer T Cells Trigger an Innate Allergic Immune Response in Atopic Dermatitis." The Journal of Allergy and Clinical Immunology, vol. 126, no. 2, 2010, pp. 290-9, 299.e1-4.
Wu WH, Park CO, Oh SH, et al. Thymic stromal lymphopoietin-activated invariant natural killer T cells trigger an innate allergic immune response in atopic dermatitis. J Allergy Clin Immunol. 2010;126(2):290-9, 299.e1-4.
Wu, W. H., Park, C. O., Oh, S. H., Kim, H. J., Kwon, Y. S., Bae, B. G., Noh, J. Y., & Lee, K. H. (2010). Thymic stromal lymphopoietin-activated invariant natural killer T cells trigger an innate allergic immune response in atopic dermatitis. The Journal of Allergy and Clinical Immunology, 126(2), 290-9, e1-4. https://doi.org/10.1016/j.jaci.2010.05.024
Wu WH, et al. Thymic Stromal Lymphopoietin-activated Invariant Natural Killer T Cells Trigger an Innate Allergic Immune Response in Atopic Dermatitis. J Allergy Clin Immunol. 2010;126(2):290-9, 299.e1-4. PubMed PMID: 20624642.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Thymic stromal lymphopoietin-activated invariant natural killer T cells trigger an innate allergic immune response in atopic dermatitis. AU - Wu,Wen Hao, AU - Park,Chang Ook, AU - Oh,Sang Ho, AU - Kim,Hee Jung, AU - Kwon,Yeon Sook, AU - Bae,Byung Gi, AU - Noh,Ji Yeon, AU - Lee,Kwang Hoon, Y1 - 2010/07/10/ PY - 2009/09/24/received PY - 2010/05/08/revised PY - 2010/05/21/accepted PY - 2010/7/14/entrez PY - 2010/7/14/pubmed PY - 2010/9/8/medline SP - 290-9, 299.e1-4 JF - The Journal of allergy and clinical immunology JO - J. Allergy Clin. Immunol. VL - 126 IS - 2 N2 - BACKGROUND: Although invariant natural killer T (iNKT) cells have been shown to play a critical role in the pathogenesis of asthma, the role of iNKT cells in atopic dermatitis (AD) has not been well evaluated. OBJECTIVE: We investigated whether iNKT cells in patients with AD increased and whether iNKT cells were activated by thymic stromal lymphopoietin (TSLP), which is highly expressed in keratinocytes of AD. METHODS: We assessed the population of iNKT cells in PBMCs of patients with AD and healthy controls (HCs) using flow cytometry. Immunohistochemistry was used to evaluate iNKT cells and TSLP expression in AD and HC skin. We also evaluated whether iNKT cells expressed the TSLP receptor, the effects of TSLP on iNKT cells, and iNKT cell-dendritic cell interactions in a TSLP-rich environment. RESULTS: There were more iNKT cells among PBMCs of patients with moderate to severe AD than mild AD (P < .05) and HC (P < .001). The number of iNKT cells was significantly larger in severe AD skin lesions than in mild (P < .001) or moderate AD skin lesions (P < .05). TSLP expression increased in lesional skin (P < .001) but not in the sera of patients with AD (P = .729) compared with HC. iNKT cells expressed TSLP receptor protein and mRNA. TSLP directly activated iNKT cells to secrete IL-4 and IL-13, and the concurrent addition of dendritic cells further activated IFN-gamma expression. CONCLUSION: Increased iNKT cells activated by TSLP, especially in patients with severe AD, might play an essential role in the innate allergic immune response in AD. SN - 1097-6825 UR - https://www.unboundmedicine.com/medline/citation/20624642/Thymic_stromal_lymphopoietin_activated_invariant_natural_killer_T_cells_trigger_an_innate_allergic_immune_response_in_atopic_dermatitis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091-6749(10)00849-3 DB - PRIME DP - Unbound Medicine ER -