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[Gene chips-aided analysis on the profiles of hippocampal whole-genome expression in depression rats following electroacupuncture treatment].
Zhen Ci Yan Jiu. 2010 Apr; 35(2):83-9.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) on the profiles of hippocampal whole-genome expression in depression rats so as to study its underlying mechanism in relieving depression.

METHODS

Thirty male SD rats were randomized into normal, model and EA groups (n = 10/group). Depression model was established by chronic unpredictable stress stimulation (forced ice-water swimming, electric shock, tail-clamping, etc.) and lonely raising for 21 days. EA (2 Hz, 1 mA) was applied to "Baihui"(GV 20) and "Yintang" (EX-HN 3) for 15 min, once daily for 7 days. The rats' ethological activities (horizontal and vertical movements) were detected with Open-field test in Morris labyrinth, and 1% sucrose solution consumption was detected. After termination of the treatment, the rats were killed and their hippocampal tissues were taken out for analyzing the whole-genome expression with Roche-NimbleGen chips (Rattus norvegicus 12 x 135 Karray).

RESULTS

Compared with normal group, the distance of horizontal movement, rearing times, and sucrose consumption at day 21 and day 28 of model group decreased considerably (P < 0.01). In comparison with model group, the sucrose consumption at day 28 in EA group increased significantly (P < 0.05). Results of gene expression microarray displayed that compared with normal group, of the down-regulated and up-regulated genes due to modeling, 101 and 125 turned to normal in their expression, respectively following EA treatment. The down-regulated genes involve energy and substance metabolism, protein biosynthesis, etc., and the up-regulated genes involve blood clotting, immunity, inflammation, apoptosis, etc.

CONCLUSION

EA of GV 20 and EX-HN 3 can normalize chronic stress stimulation induced down- and up-regulated expression of many genes in depression rats, which may contribute to its clinical effect in relieving depression.

Authors+Show Affiliations

Key Laboratory for Study on the Integration of Acupuncture and Medication, Nanjing University of Chinese Medicine, Nanjing 210029, China. birddingdang@yahoo.com.cnNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

20626138

Citation

Jiang, Li-hua, and Ling-ling Wang. "[Gene Chips-aided Analysis On the Profiles of Hippocampal Whole-genome Expression in Depression Rats Following Electroacupuncture Treatment]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 35, no. 2, 2010, pp. 83-9.
Jiang LH, Wang LL. [Gene chips-aided analysis on the profiles of hippocampal whole-genome expression in depression rats following electroacupuncture treatment]. Zhen Ci Yan Jiu. 2010;35(2):83-9.
Jiang, L. H., & Wang, L. L. (2010). [Gene chips-aided analysis on the profiles of hippocampal whole-genome expression in depression rats following electroacupuncture treatment]. Zhen Ci Yan Jiu = Acupuncture Research, 35(2), 83-9.
Jiang LH, Wang LL. [Gene Chips-aided Analysis On the Profiles of Hippocampal Whole-genome Expression in Depression Rats Following Electroacupuncture Treatment]. Zhen Ci Yan Jiu. 2010;35(2):83-9. PubMed PMID: 20626138.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Gene chips-aided analysis on the profiles of hippocampal whole-genome expression in depression rats following electroacupuncture treatment]. AU - Jiang,Li-hua, AU - Wang,Ling-ling, PY - 2010/7/15/entrez PY - 2010/7/16/pubmed PY - 2010/8/18/medline SP - 83 EP - 9 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 35 IS - 2 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) on the profiles of hippocampal whole-genome expression in depression rats so as to study its underlying mechanism in relieving depression. METHODS: Thirty male SD rats were randomized into normal, model and EA groups (n = 10/group). Depression model was established by chronic unpredictable stress stimulation (forced ice-water swimming, electric shock, tail-clamping, etc.) and lonely raising for 21 days. EA (2 Hz, 1 mA) was applied to "Baihui"(GV 20) and "Yintang" (EX-HN 3) for 15 min, once daily for 7 days. The rats' ethological activities (horizontal and vertical movements) were detected with Open-field test in Morris labyrinth, and 1% sucrose solution consumption was detected. After termination of the treatment, the rats were killed and their hippocampal tissues were taken out for analyzing the whole-genome expression with Roche-NimbleGen chips (Rattus norvegicus 12 x 135 Karray). RESULTS: Compared with normal group, the distance of horizontal movement, rearing times, and sucrose consumption at day 21 and day 28 of model group decreased considerably (P < 0.01). In comparison with model group, the sucrose consumption at day 28 in EA group increased significantly (P < 0.05). Results of gene expression microarray displayed that compared with normal group, of the down-regulated and up-regulated genes due to modeling, 101 and 125 turned to normal in their expression, respectively following EA treatment. The down-regulated genes involve energy and substance metabolism, protein biosynthesis, etc., and the up-regulated genes involve blood clotting, immunity, inflammation, apoptosis, etc. CONCLUSION: EA of GV 20 and EX-HN 3 can normalize chronic stress stimulation induced down- and up-regulated expression of many genes in depression rats, which may contribute to its clinical effect in relieving depression. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/20626138/[Gene_chips_aided_analysis_on_the_profiles_of_hippocampal_whole_genome_expression_in_depression_rats_following_electroacupuncture_treatment]_ L2 - http://www.diseaseinfosearch.org/result/2199 DB - PRIME DP - Unbound Medicine ER -