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High cell-density processes in batch mode of a genetically engineered Escherichia coli strain with minimized overflow metabolism using a pressurized bioreactor.
J Biotechnol. 2010 Oct 01; 150(1):73-9.JB

Abstract

A common method to minimize overflow metabolism and to enable high cell-density is to operate microbial processes in fed-batch mode under carbon-limiting conditions. This requires sophisticated process control schemes with expensive hardware equipment and software and well-characterized processes parameters. To generate high-cell density, a more simplified strategy would be beneficial. Therefore, a genetically engineered Escherichia coli strain with a modified glucose uptake system was cultivated in batch mode. In the applied strain, the usual phosphotransferase system of a K12-derived strain was inactivated, while the galactose permease system was amplified. Upon cultivating this E. coli strain in pure minimal media, the acetate concentration did not exceed values of 0.35 g L(-1), even when the batch fermentation was started with a glucose concentration of 130 g L(-1). Finally, maximum biomass concentrations of 48 g L(-1) dry cell weight and maximum space-time yields of 2.10 g L(-1) h(-1) were reached. To provide an unlimited growth under fully aerobic conditions (DOT>30%) at comparatively low values for specific power input (3-4 kW m(-3)), a pressurized bioreactor was used. Consequentially, to our knowledge, this study using a bioreactor with elevated headspace pressure generate the highest oxygen transfer rate (451 mmol L(-1) h(-1)) ever reached in batch cultivations.

Authors+Show Affiliations

RWTH Aachen, AVT--Biochemical Engineering, Worringer Weg 1, 52056 Aachen, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20630485

Citation

Knabben, Ingo, et al. "High Cell-density Processes in Batch Mode of a Genetically Engineered Escherichia Coli Strain With Minimized Overflow Metabolism Using a Pressurized Bioreactor." Journal of Biotechnology, vol. 150, no. 1, 2010, pp. 73-9.
Knabben I, Regestein L, Marquering F, et al. High cell-density processes in batch mode of a genetically engineered Escherichia coli strain with minimized overflow metabolism using a pressurized bioreactor. J Biotechnol. 2010;150(1):73-9.
Knabben, I., Regestein, L., Marquering, F., Steinbusch, S., Lara, A. R., & Büchs, J. (2010). High cell-density processes in batch mode of a genetically engineered Escherichia coli strain with minimized overflow metabolism using a pressurized bioreactor. Journal of Biotechnology, 150(1), 73-9. https://doi.org/10.1016/j.jbiotec.2010.07.006
Knabben I, et al. High Cell-density Processes in Batch Mode of a Genetically Engineered Escherichia Coli Strain With Minimized Overflow Metabolism Using a Pressurized Bioreactor. J Biotechnol. 2010 Oct 1;150(1):73-9. PubMed PMID: 20630485.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High cell-density processes in batch mode of a genetically engineered Escherichia coli strain with minimized overflow metabolism using a pressurized bioreactor. AU - Knabben,Ingo, AU - Regestein,Lars, AU - Marquering,Frank, AU - Steinbusch,Sven, AU - Lara,Alvaro R, AU - Büchs,Jochen, Y1 - 2010/07/12/ PY - 2010/03/16/received PY - 2010/06/04/revised PY - 2010/07/06/accepted PY - 2010/7/16/entrez PY - 2010/7/16/pubmed PY - 2011/2/9/medline SP - 73 EP - 9 JF - Journal of biotechnology JO - J. Biotechnol. VL - 150 IS - 1 N2 - A common method to minimize overflow metabolism and to enable high cell-density is to operate microbial processes in fed-batch mode under carbon-limiting conditions. This requires sophisticated process control schemes with expensive hardware equipment and software and well-characterized processes parameters. To generate high-cell density, a more simplified strategy would be beneficial. Therefore, a genetically engineered Escherichia coli strain with a modified glucose uptake system was cultivated in batch mode. In the applied strain, the usual phosphotransferase system of a K12-derived strain was inactivated, while the galactose permease system was amplified. Upon cultivating this E. coli strain in pure minimal media, the acetate concentration did not exceed values of 0.35 g L(-1), even when the batch fermentation was started with a glucose concentration of 130 g L(-1). Finally, maximum biomass concentrations of 48 g L(-1) dry cell weight and maximum space-time yields of 2.10 g L(-1) h(-1) were reached. To provide an unlimited growth under fully aerobic conditions (DOT>30%) at comparatively low values for specific power input (3-4 kW m(-3)), a pressurized bioreactor was used. Consequentially, to our knowledge, this study using a bioreactor with elevated headspace pressure generate the highest oxygen transfer rate (451 mmol L(-1) h(-1)) ever reached in batch cultivations. SN - 1873-4863 UR - https://www.unboundmedicine.com/medline/citation/20630485/High_cell_density_processes_in_batch_mode_of_a_genetically_engineered_Escherichia_coli_strain_with_minimized_overflow_metabolism_using_a_pressurized_bioreactor_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1656(10)00305-6 DB - PRIME DP - Unbound Medicine ER -