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RNA secondary structure repression of a muscle-specific exon in HeLa cell nuclear extracts.
Science. 1991 Jun 28; 252(5014):1823-8.Sci

Abstract

The chicken beta-tropomyosin pre-messenger RNA (pre-mRNA) is spliced in a tissue-specific manner to yield messenger RNA's (mRNA's) coding for different isoforms of this protein. Exons 6A and 6B are spliced in a mutually exclusive manner; exon 6B was included in skeletal muscle, whereas exon 6A was preferred in all other tissues. The distal portion of the intron upstream of exon 6B was shown to form stable double-stranded regions with part of the intron downstream of exon 6B and with sequences in exon 6B. This structure repressed splicing of exon 6B to exon 7 in a HeLa cell extract. Derepression of splicing occurred on disruption of this structure and repression followed when the structure was re-formed, even if the structure was formed between two different RNA molecules. Repression leads to inhibition of formation of spliceosomes. Disrupting either of the two double-stranded regions could lead to derepression, whereas re-forming the helices by suppressor mutations reestablished repression. These results support a simple model of tissue-specific splicing in this region of the pre-mRNA.

Authors+Show Affiliations

Centre de Génétique Moléculaire, C.N.R.S., Université de Paris VI, Gif-sur-Yvette, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2063195

Citation

Clouet d'Orval, B, et al. "RNA Secondary Structure Repression of a Muscle-specific Exon in HeLa Cell Nuclear Extracts." Science (New York, N.Y.), vol. 252, no. 5014, 1991, pp. 1823-8.
Clouet d'Orval B, d'Aubenton Carafa Y, Sirand-Pugnet P, et al. RNA secondary structure repression of a muscle-specific exon in HeLa cell nuclear extracts. Science. 1991;252(5014):1823-8.
Clouet d'Orval, B., d'Aubenton Carafa, Y., Sirand-Pugnet, P., Gallego, M., Brody, E., & Marie, J. (1991). RNA secondary structure repression of a muscle-specific exon in HeLa cell nuclear extracts. Science (New York, N.Y.), 252(5014), 1823-8.
Clouet d'Orval B, et al. RNA Secondary Structure Repression of a Muscle-specific Exon in HeLa Cell Nuclear Extracts. Science. 1991 Jun 28;252(5014):1823-8. PubMed PMID: 2063195.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RNA secondary structure repression of a muscle-specific exon in HeLa cell nuclear extracts. AU - Clouet d'Orval,B, AU - d'Aubenton Carafa,Y, AU - Sirand-Pugnet,P, AU - Gallego,M, AU - Brody,E, AU - Marie,J, PY - 1991/6/28/pubmed PY - 1991/6/28/medline PY - 1991/6/28/entrez SP - 1823 EP - 8 JF - Science (New York, N.Y.) JO - Science VL - 252 IS - 5014 N2 - The chicken beta-tropomyosin pre-messenger RNA (pre-mRNA) is spliced in a tissue-specific manner to yield messenger RNA's (mRNA's) coding for different isoforms of this protein. Exons 6A and 6B are spliced in a mutually exclusive manner; exon 6B was included in skeletal muscle, whereas exon 6A was preferred in all other tissues. The distal portion of the intron upstream of exon 6B was shown to form stable double-stranded regions with part of the intron downstream of exon 6B and with sequences in exon 6B. This structure repressed splicing of exon 6B to exon 7 in a HeLa cell extract. Derepression of splicing occurred on disruption of this structure and repression followed when the structure was re-formed, even if the structure was formed between two different RNA molecules. Repression leads to inhibition of formation of spliceosomes. Disrupting either of the two double-stranded regions could lead to derepression, whereas re-forming the helices by suppressor mutations reestablished repression. These results support a simple model of tissue-specific splicing in this region of the pre-mRNA. SN - 0036-8075 UR - https://www.unboundmedicine.com/medline/citation/2063195/RNA_secondary_structure_repression_of_a_muscle_specific_exon_in_HeLa_cell_nuclear_extracts_ L2 - https://www.sciencemag.org/cgi/pmidlookup?view=long&pmid=2063195 DB - PRIME DP - Unbound Medicine ER -