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Anti-inflammatory, antioxidant, anti-tyrosinase and phenolic contents of four Podocarpus species used in traditional medicine in South Africa.
J Ethnopharmacol. 2011 Jul 14; 136(3):496-503.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Species of Podocarpus are used traditionally in their native areas for the treatment of fevers, asthma, coughs, cholera, chest complaints, arthritis, rheumatism, venereal diseases and distemper in dogs.

AIMS OF THE STUDY

To investigate the antioxidant, anti-inflammatory and anti-tyrosinase activities of four Podocarpus species, Podocarpus elongatus, Podocarpus falcatus, Podocarpus henkelii and Podocarpus latifolius, used in traditional medicine in South Africa. Phytochemical analysis to determine the phenolic contents was also carried out.

MATERIALS AND METHODS

DPPH, FRAP and β-carotene-linoleic acid assays were used to determine the antioxidant/radical scavenging activities of these species. Anti-inflammatory activity of these species was assayed against two cyclooxygenase enzymes (COX-1 and COX-2). Tyrosinase inhibition activity was analysed using the modified dopachrome method with l-DOPA as the substrate. Phenolics were quantitatively determined using spectrophotometric methods.

RESULTS

Stems of Podocarpus latifolius exhibited the lowest EC(50) (0.84 μg/ml) inhibition against DPPH. The percentage antioxidant activity based on the bleaching rate of β-carotene ranged from 96% to 99%. High ferric reducing power was observed in all the extracts. For COX-1, the lowest EC(50) value was exhibited by stem extracts of Podocarpus elongatus (5.02 μg/ml) and leaf extract of Podocarpus latifolius showed the lowest EC(50) against COX-2 (5.13 μg/ml). All extracts inhibited tyrosinase activity in a dose-dependent manner with stem extract of Podocarpus elongatus being the most potent with an EC(50) value of 0.14 mg/ml. The total phenolic content ranged from 2.38 to 6.94 mg of GAE/g dry sample.

CONCLUSION

The significant pharmacological activities observed support the use of these species in traditional medicine and may also be candidates in the search for modern pharmaceuticals in medicine, food and cosmetic industries.

Authors+Show Affiliations

Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Scottsville, South Africa.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20633623

Citation

Abdillahi, H S., et al. "Anti-inflammatory, Antioxidant, Anti-tyrosinase and Phenolic Contents of Four Podocarpus Species Used in Traditional Medicine in South Africa." Journal of Ethnopharmacology, vol. 136, no. 3, 2011, pp. 496-503.
Abdillahi HS, Finnie JF, Van Staden J. Anti-inflammatory, antioxidant, anti-tyrosinase and phenolic contents of four Podocarpus species used in traditional medicine in South Africa. J Ethnopharmacol. 2011;136(3):496-503.
Abdillahi, H. S., Finnie, J. F., & Van Staden, J. (2011). Anti-inflammatory, antioxidant, anti-tyrosinase and phenolic contents of four Podocarpus species used in traditional medicine in South Africa. Journal of Ethnopharmacology, 136(3), 496-503. https://doi.org/10.1016/j.jep.2010.07.019
Abdillahi HS, Finnie JF, Van Staden J. Anti-inflammatory, Antioxidant, Anti-tyrosinase and Phenolic Contents of Four Podocarpus Species Used in Traditional Medicine in South Africa. J Ethnopharmacol. 2011 Jul 14;136(3):496-503. PubMed PMID: 20633623.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-inflammatory, antioxidant, anti-tyrosinase and phenolic contents of four Podocarpus species used in traditional medicine in South Africa. AU - Abdillahi,H S, AU - Finnie,J F, AU - Van Staden,J, Y1 - 2010/07/13/ PY - 2010/04/13/received PY - 2010/06/28/revised PY - 2010/07/06/accepted PY - 2010/7/17/entrez PY - 2010/7/17/pubmed PY - 2012/1/11/medline SP - 496 EP - 503 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 136 IS - 3 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Species of Podocarpus are used traditionally in their native areas for the treatment of fevers, asthma, coughs, cholera, chest complaints, arthritis, rheumatism, venereal diseases and distemper in dogs. AIMS OF THE STUDY: To investigate the antioxidant, anti-inflammatory and anti-tyrosinase activities of four Podocarpus species, Podocarpus elongatus, Podocarpus falcatus, Podocarpus henkelii and Podocarpus latifolius, used in traditional medicine in South Africa. Phytochemical analysis to determine the phenolic contents was also carried out. MATERIALS AND METHODS: DPPH, FRAP and β-carotene-linoleic acid assays were used to determine the antioxidant/radical scavenging activities of these species. Anti-inflammatory activity of these species was assayed against two cyclooxygenase enzymes (COX-1 and COX-2). Tyrosinase inhibition activity was analysed using the modified dopachrome method with l-DOPA as the substrate. Phenolics were quantitatively determined using spectrophotometric methods. RESULTS: Stems of Podocarpus latifolius exhibited the lowest EC(50) (0.84 μg/ml) inhibition against DPPH. The percentage antioxidant activity based on the bleaching rate of β-carotene ranged from 96% to 99%. High ferric reducing power was observed in all the extracts. For COX-1, the lowest EC(50) value was exhibited by stem extracts of Podocarpus elongatus (5.02 μg/ml) and leaf extract of Podocarpus latifolius showed the lowest EC(50) against COX-2 (5.13 μg/ml). All extracts inhibited tyrosinase activity in a dose-dependent manner with stem extract of Podocarpus elongatus being the most potent with an EC(50) value of 0.14 mg/ml. The total phenolic content ranged from 2.38 to 6.94 mg of GAE/g dry sample. CONCLUSION: The significant pharmacological activities observed support the use of these species in traditional medicine and may also be candidates in the search for modern pharmaceuticals in medicine, food and cosmetic industries. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/20633623/Anti_inflammatory_antioxidant_anti_tyrosinase_and_phenolic_contents_of_four_Podocarpus_species_used_in_traditional_medicine_in_South_Africa_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(10)00481-2 DB - PRIME DP - Unbound Medicine ER -