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Neuroprotective effects of an herbal medicine, Yi-Gan San on MPP+/MPTP-induced cytotoxicity in vitro and in vivo.
J Ethnopharmacol. 2010 Sep 15; 131(2):433-42.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

A traditional herb, Yi-Gan San, has been widely used for the management of neurodegenerative disorders in traditional East Asian Medicine.

AIM OF THE STUDY

The present study investigated the neuroprotective effects of Yi-Gan San in 1-methyl-4-phenylpyridine/1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced cytotoxicity in vitro and in vivo and sought to clarify its underlying mechanisms.

MATERIALS AND METHODS

The effect of Yi-Gan San on 1-methyl-4-phenylpyridine was measured in terms of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assays, caspase-3 activity, and western blot analysis of phosphorylated Akt, one of the survival-related signaling proteins in SH-SY5Y cells. The effects of Yi-Gan San were also confirmed in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinsonian mouse model using a rotarod test and tyrosine hydroxylase-immunohistochemistry.

RESULTS

Pretreatment of Yi-Gan San with 1-methyl-4-phenylpyridine showed a significant protective effect on SH-SY5Y cells and significantly decreased the level of caspase-3 activity compared to the values for the 1-methyl-4-phenylpyridine-treated cells. This process increased the protein expressions of phosphorylated Akt, and an inhibitor of phosphatidylinositol 3-kinase (PI3K)/Akt, LY294002, significantly decreased this protective effect of Yi-Gan San. In the mouse Parkinson's disease model, treatment with Yi-Gan San also significantly improved motor functioning and prevented dopaminergic loss related to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine challenge.

CONCLUSION

Using both in vitro and in vivo methods, this study revealed that Yi-Gan San has neuroprotective effects and rescues dopaminergic neurons from 1-methyl-4-phenylpyridine/1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine toxicity, possibly via the PI3K/Akt pathway.

Authors+Show Affiliations

Studies of Translational Acupuncture Research (STAR), Acupuncture & Meridian Science Research Center (AMSRC), Kyung Hee University, Seoul, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20633628

Citation

Doo, Ah-Reum, et al. "Neuroprotective Effects of an Herbal Medicine, Yi-Gan San On MPP+/MPTP-induced Cytotoxicity in Vitro and in Vivo." Journal of Ethnopharmacology, vol. 131, no. 2, 2010, pp. 433-42.
Doo AR, Kim SN, Park JY, et al. Neuroprotective effects of an herbal medicine, Yi-Gan San on MPP+/MPTP-induced cytotoxicity in vitro and in vivo. J Ethnopharmacol. 2010;131(2):433-42.
Doo, A. R., Kim, S. N., Park, J. Y., Cho, K. H., Hong, J., Eun-Kyung, K., Moon, S. K., Jung, W. S., Lee, H., Jung, J. H., & Park, H. J. (2010). Neuroprotective effects of an herbal medicine, Yi-Gan San on MPP+/MPTP-induced cytotoxicity in vitro and in vivo. Journal of Ethnopharmacology, 131(2), 433-42. https://doi.org/10.1016/j.jep.2010.07.008
Doo AR, et al. Neuroprotective Effects of an Herbal Medicine, Yi-Gan San On MPP+/MPTP-induced Cytotoxicity in Vitro and in Vivo. J Ethnopharmacol. 2010 Sep 15;131(2):433-42. PubMed PMID: 20633628.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuroprotective effects of an herbal medicine, Yi-Gan San on MPP+/MPTP-induced cytotoxicity in vitro and in vivo. AU - Doo,Ah-Reum, AU - Kim,Seung-Nam, AU - Park,Ji-Yeon, AU - Cho,Ki Ho, AU - Hong,Jongki, AU - Eun-Kyung,Kim, AU - Moon,Sang Kwan, AU - Jung,Woo Sang, AU - Lee,Hyejung, AU - Jung,Jae Han, AU - Park,Hi-Joon, Y1 - 2010/07/13/ PY - 2010/02/17/received PY - 2010/05/27/revised PY - 2010/07/06/accepted PY - 2010/7/17/entrez PY - 2010/7/17/pubmed PY - 2011/5/3/medline SP - 433 EP - 42 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 131 IS - 2 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: A traditional herb, Yi-Gan San, has been widely used for the management of neurodegenerative disorders in traditional East Asian Medicine. AIM OF THE STUDY: The present study investigated the neuroprotective effects of Yi-Gan San in 1-methyl-4-phenylpyridine/1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced cytotoxicity in vitro and in vivo and sought to clarify its underlying mechanisms. MATERIALS AND METHODS: The effect of Yi-Gan San on 1-methyl-4-phenylpyridine was measured in terms of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assays, caspase-3 activity, and western blot analysis of phosphorylated Akt, one of the survival-related signaling proteins in SH-SY5Y cells. The effects of Yi-Gan San were also confirmed in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinsonian mouse model using a rotarod test and tyrosine hydroxylase-immunohistochemistry. RESULTS: Pretreatment of Yi-Gan San with 1-methyl-4-phenylpyridine showed a significant protective effect on SH-SY5Y cells and significantly decreased the level of caspase-3 activity compared to the values for the 1-methyl-4-phenylpyridine-treated cells. This process increased the protein expressions of phosphorylated Akt, and an inhibitor of phosphatidylinositol 3-kinase (PI3K)/Akt, LY294002, significantly decreased this protective effect of Yi-Gan San. In the mouse Parkinson's disease model, treatment with Yi-Gan San also significantly improved motor functioning and prevented dopaminergic loss related to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine challenge. CONCLUSION: Using both in vitro and in vivo methods, this study revealed that Yi-Gan San has neuroprotective effects and rescues dopaminergic neurons from 1-methyl-4-phenylpyridine/1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine toxicity, possibly via the PI3K/Akt pathway. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/20633628/Neuroprotective_effects_of_an_herbal_medicine_Yi_Gan_San_on_MPP+/MPTP_induced_cytotoxicity_in_vitro_and_in_vivo_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(10)00470-8 DB - PRIME DP - Unbound Medicine ER -