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CspC regulates rpoS transcript levels and complements hfq deletions.
Res Microbiol. 2010 Oct; 161(8):694-700.RM

Abstract

The general stress response in Escherichia coli is activated by several stress agents, including entering the stationary growth phase. This response constitutes a complex regulatory network in which a large number of genes are induced and others are repressed. The stress response is regulated by the alternative sigma factor σ(S) encoded by the rpoS gene. The rpoS transcripts are substrates of the RNA binding protein, Hfq, which is essential for its translation. The rpoS mRNA is also a substrate of the cold shock protein C (CspC) which stabilizes the transcripts. Here we demonstrate, using pull-down assays, that CspC interacts with Hfq via mRNA molecules. We also show that CspC acts on the 5' UTR of the rpoS transcript, but its activity on rpoS is independent of Hfq. Moreover, we show that CspC suppresses the phenotypes of an hfq deletion. These results elucidate a new aspect in the post-transcriptional regulation of the stress response and will further our understanding of this complex network.

Authors+Show Affiliations

Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Tel Aviv 69978, Israel.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20633642

Citation

Cohen-Or, Ifat, et al. "CspC Regulates rpoS Transcript Levels and Complements Hfq Deletions." Research in Microbiology, vol. 161, no. 8, 2010, pp. 694-700.
Cohen-Or I, Shenhar Y, Biran D, et al. CspC regulates rpoS transcript levels and complements hfq deletions. Res Microbiol. 2010;161(8):694-700.
Cohen-Or, I., Shenhar, Y., Biran, D., & Ron, E. Z. (2010). CspC regulates rpoS transcript levels and complements hfq deletions. Research in Microbiology, 161(8), 694-700. https://doi.org/10.1016/j.resmic.2010.06.009
Cohen-Or I, et al. CspC Regulates rpoS Transcript Levels and Complements Hfq Deletions. Res Microbiol. 2010;161(8):694-700. PubMed PMID: 20633642.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CspC regulates rpoS transcript levels and complements hfq deletions. AU - Cohen-Or,Ifat, AU - Shenhar,Yotam, AU - Biran,Dvora, AU - Ron,Eliora Z, Y1 - 2010/07/13/ PY - 2010/05/02/received PY - 2010/06/21/revised PY - 2010/06/28/accepted PY - 2010/7/17/entrez PY - 2010/7/17/pubmed PY - 2010/12/16/medline SP - 694 EP - 700 JF - Research in microbiology JO - Res Microbiol VL - 161 IS - 8 N2 - The general stress response in Escherichia coli is activated by several stress agents, including entering the stationary growth phase. This response constitutes a complex regulatory network in which a large number of genes are induced and others are repressed. The stress response is regulated by the alternative sigma factor σ(S) encoded by the rpoS gene. The rpoS transcripts are substrates of the RNA binding protein, Hfq, which is essential for its translation. The rpoS mRNA is also a substrate of the cold shock protein C (CspC) which stabilizes the transcripts. Here we demonstrate, using pull-down assays, that CspC interacts with Hfq via mRNA molecules. We also show that CspC acts on the 5' UTR of the rpoS transcript, but its activity on rpoS is independent of Hfq. Moreover, we show that CspC suppresses the phenotypes of an hfq deletion. These results elucidate a new aspect in the post-transcriptional regulation of the stress response and will further our understanding of this complex network. SN - 1769-7123 UR - https://www.unboundmedicine.com/medline/citation/20633642/CspC_regulates_rpoS_transcript_levels_and_complements_hfq_deletions_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0923-2508(10)00155-5 DB - PRIME DP - Unbound Medicine ER -