Tags

Type your tag names separated by a space and hit enter

TOR and PKA pathways synergize at the level of the Ste11 transcription factor to prevent mating and meiosis in fission yeast.
PLoS One. 2010 Jul 09; 5(7):e11514.Plos

Abstract

BACKGROUND

In the fission yeast Schizosaccharomyces pombe, the TOR (target of rapamycin) and PKA (protein kinase A) signaling transduction pathways regulate the expression of genes required for cell growth and sexual differentiation in response to the nutritional environment. Inhibition of Tor2 signaling results in the induction of genes involved in sexual differentiation, and the cells undergo mating and meiosis, even under good nutritional conditions. The same phenotype is observed in mutants in which the PKA pathway is inactive. By contrast, Tor2 overexpression or mutations that hyperactivate PKA signaling impair sexual differentiation, even under poor nutritional conditions. Accordingly, a very important question is to understand the molecular mechanism by which these two pathways coordinately regulate gene expression in response to nutrients.

METHODOLOGY/PRINCIPAL FINDINGS

Here we demonstrate that TOR and PKA pathways operate coordinately to negatively regulate sexual differentiation by inhibiting the nuclear accumulation of the Ste11 transcription factor. However, the Tor2 pathway is unable to block the nuclear localization of Ste11 under good nutritional conditions when the PKA pathway is inactive. Using microarray analyses, we found that both pathways inhibit sexual differentiation by blocking ste11-dependent gene expression.

CONCLUSIONS/SIGNIFICANCE

We conclude that both the PKA and the TOR pathways inhibit Ste11 nuclear accumulation to repress Ste11-dependent gene expression. However, the PKA pathway plays a quantitatively more important role than the TOR pathway in this process.

Authors+Show Affiliations

Instituto de Biología Molecular y Celular del Cáncer, Consejo Superior de Investigaciones Científicas, Salamanca University, Salamanca, Spain.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20634885

Citation

Valbuena, Noelia, and Sergio Moreno. "TOR and PKA Pathways Synergize at the Level of the Ste11 Transcription Factor to Prevent Mating and Meiosis in Fission Yeast." PloS One, vol. 5, no. 7, 2010, pp. e11514.
Valbuena N, Moreno S. TOR and PKA pathways synergize at the level of the Ste11 transcription factor to prevent mating and meiosis in fission yeast. PLoS One. 2010;5(7):e11514.
Valbuena, N., & Moreno, S. (2010). TOR and PKA pathways synergize at the level of the Ste11 transcription factor to prevent mating and meiosis in fission yeast. PloS One, 5(7), e11514. https://doi.org/10.1371/journal.pone.0011514
Valbuena N, Moreno S. TOR and PKA Pathways Synergize at the Level of the Ste11 Transcription Factor to Prevent Mating and Meiosis in Fission Yeast. PLoS One. 2010 Jul 9;5(7):e11514. PubMed PMID: 20634885.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TOR and PKA pathways synergize at the level of the Ste11 transcription factor to prevent mating and meiosis in fission yeast. AU - Valbuena,Noelia, AU - Moreno,Sergio, Y1 - 2010/07/09/ PY - 2010/04/27/received PY - 2010/06/16/accepted PY - 2010/7/17/entrez PY - 2010/7/17/pubmed PY - 2010/10/29/medline SP - e11514 EP - e11514 JF - PloS one JO - PLoS One VL - 5 IS - 7 N2 - BACKGROUND: In the fission yeast Schizosaccharomyces pombe, the TOR (target of rapamycin) and PKA (protein kinase A) signaling transduction pathways regulate the expression of genes required for cell growth and sexual differentiation in response to the nutritional environment. Inhibition of Tor2 signaling results in the induction of genes involved in sexual differentiation, and the cells undergo mating and meiosis, even under good nutritional conditions. The same phenotype is observed in mutants in which the PKA pathway is inactive. By contrast, Tor2 overexpression or mutations that hyperactivate PKA signaling impair sexual differentiation, even under poor nutritional conditions. Accordingly, a very important question is to understand the molecular mechanism by which these two pathways coordinately regulate gene expression in response to nutrients. METHODOLOGY/PRINCIPAL FINDINGS: Here we demonstrate that TOR and PKA pathways operate coordinately to negatively regulate sexual differentiation by inhibiting the nuclear accumulation of the Ste11 transcription factor. However, the Tor2 pathway is unable to block the nuclear localization of Ste11 under good nutritional conditions when the PKA pathway is inactive. Using microarray analyses, we found that both pathways inhibit sexual differentiation by blocking ste11-dependent gene expression. CONCLUSIONS/SIGNIFICANCE: We conclude that both the PKA and the TOR pathways inhibit Ste11 nuclear accumulation to repress Ste11-dependent gene expression. However, the PKA pathway plays a quantitatively more important role than the TOR pathway in this process. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/20634885/TOR_and_PKA_pathways_synergize_at_the_level_of_the_Ste11_transcription_factor_to_prevent_mating_and_meiosis_in_fission_yeast_ L2 - https://dx.plos.org/10.1371/journal.pone.0011514 DB - PRIME DP - Unbound Medicine ER -