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Leukotriene B4 and 8-isoprostane in exhaled breath condensate of children with episodic and persistent asthma.
J Investig Allergol Clin Immunol. 2010; 20(3):237-43.JI

Abstract

BACKGROUND

Leukotrienes and isoprostanes are biomarkers of airway inflammation and oxidative stress that can be detected in exhaled breath condensate (EBC). The aim of this study was to evaluate leukotriene B4 (LTB4) and 8-isoprostane levels in EBC of healthy and asthmatic children with episodic and moderate persistent asthma.

METHODS

EBC was collected from 62 children aged 6 to 14 years: 22 healthy children, 30 patients with episodic asthma, and 10 patients with moderate persistent asthma, without preventive treatment at the time of enrolment.

RESULTS

LTB concentrations were higher in children with asthma than in healthy controls (50.7 pg/mL vs. 13.68 pg/mL, P < .011). The same was true for children with moderate persistent asthma compared to children with episodic asthma (146.9 pg/mL vs. 18.85 pg/mL, P < .0001), children with moderate persistent asthma compared to healthy controls (146.9 pg/mL vs. 13.68 pg/mL, P < .0001), and children with episodic asthma compared to healthy controls (P, nonsignificant). EBC concentrations of 8-isoprostane were higher in asthmatic than in healthy children (18.3 pg/mL vs. 6.59 pg/mL, P < .026). They were also increased in children with moderate persistent asthma compared to those with episodic asthma (36.25 pg/mL and 12.28 pg/mL, P < .012), and in children with moderate persistent asthma and episodic asthma compared to healthy controls (36.25 pg/mL vs. 6.59 pg/mL [P < .0001] and 12.28 pg/mL versus 6.59 pg/mL [P < .0001], respectively).

CONCLUSION

LTB4 and 8-isoprostane concentrations were increased in asthmatic children compared to healthy individuals, with differences detected for 2 degrees of asthma severity. Our findings suggest that EBC is a noninvasive method for airway inflammation and oxidative stress assessment.

Authors+Show Affiliations

Unidad de Alergologia, Hospital General Universitario, Valencia, Spain. socaba@alumni.uv.esNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20635789

Citation

Caballero Balanzá, S, et al. "Leukotriene B4 and 8-isoprostane in Exhaled Breath Condensate of Children With Episodic and Persistent Asthma." Journal of Investigational Allergology & Clinical Immunology, vol. 20, no. 3, 2010, pp. 237-43.
Caballero Balanzá S, Martorell Aragonés A, Cerdá Mir JC, et al. Leukotriene B4 and 8-isoprostane in exhaled breath condensate of children with episodic and persistent asthma. J Investig Allergol Clin Immunol. 2010;20(3):237-43.
Caballero Balanzá, S., Martorell Aragonés, A., Cerdá Mir, J. C., Belda Ramírez, J., Ramírez, J. B., Navarro Iváñez, R., Navarro Soriano, A., Félix Toledo, R., & Escribano Montaner, A. (2010). Leukotriene B4 and 8-isoprostane in exhaled breath condensate of children with episodic and persistent asthma. Journal of Investigational Allergology & Clinical Immunology, 20(3), 237-43.
Caballero Balanzá S, et al. Leukotriene B4 and 8-isoprostane in Exhaled Breath Condensate of Children With Episodic and Persistent Asthma. J Investig Allergol Clin Immunol. 2010;20(3):237-43. PubMed PMID: 20635789.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Leukotriene B4 and 8-isoprostane in exhaled breath condensate of children with episodic and persistent asthma. AU - Caballero Balanzá,S, AU - Martorell Aragonés,A, AU - Cerdá Mir,J C, AU - Belda Ramírez,J, AU - Ramírez,J B, AU - Navarro Iváñez,R, AU - Navarro Soriano,A, AU - Félix Toledo,R, AU - Escribano Montaner,A, PY - 2010/7/20/entrez PY - 2010/7/20/pubmed PY - 2010/9/2/medline SP - 237 EP - 43 JF - Journal of investigational allergology & clinical immunology JO - J Investig Allergol Clin Immunol VL - 20 IS - 3 N2 - BACKGROUND: Leukotrienes and isoprostanes are biomarkers of airway inflammation and oxidative stress that can be detected in exhaled breath condensate (EBC). The aim of this study was to evaluate leukotriene B4 (LTB4) and 8-isoprostane levels in EBC of healthy and asthmatic children with episodic and moderate persistent asthma. METHODS: EBC was collected from 62 children aged 6 to 14 years: 22 healthy children, 30 patients with episodic asthma, and 10 patients with moderate persistent asthma, without preventive treatment at the time of enrolment. RESULTS: LTB concentrations were higher in children with asthma than in healthy controls (50.7 pg/mL vs. 13.68 pg/mL, P < .011). The same was true for children with moderate persistent asthma compared to children with episodic asthma (146.9 pg/mL vs. 18.85 pg/mL, P < .0001), children with moderate persistent asthma compared to healthy controls (146.9 pg/mL vs. 13.68 pg/mL, P < .0001), and children with episodic asthma compared to healthy controls (P, nonsignificant). EBC concentrations of 8-isoprostane were higher in asthmatic than in healthy children (18.3 pg/mL vs. 6.59 pg/mL, P < .026). They were also increased in children with moderate persistent asthma compared to those with episodic asthma (36.25 pg/mL and 12.28 pg/mL, P < .012), and in children with moderate persistent asthma and episodic asthma compared to healthy controls (36.25 pg/mL vs. 6.59 pg/mL [P < .0001] and 12.28 pg/mL versus 6.59 pg/mL [P < .0001], respectively). CONCLUSION: LTB4 and 8-isoprostane concentrations were increased in asthmatic children compared to healthy individuals, with differences detected for 2 degrees of asthma severity. Our findings suggest that EBC is a noninvasive method for airway inflammation and oxidative stress assessment. SN - 1018-9068 UR - https://www.unboundmedicine.com/medline/citation/20635789/Leukotriene_B4_and_8_isoprostane_in_exhaled_breath_condensate_of_children_with_episodic_and_persistent_asthma_ L2 - http://www.jiaci.org/issues/vol20issue3/vol20issue03-9.htm DB - PRIME DP - Unbound Medicine ER -