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A putative RNA-binding protein positively regulates salicylic acid-mediated immunity in Arabidopsis.
Mol Plant Microbe Interact. 2010 Dec; 23(12):1573-83.MP

Abstract

RNA-binding proteins (RBP) can control gene expression at both transcriptional and post-transcriptional levels. Plants respond to pathogen infection with rapid reprogramming of gene expression. However, little is known about how plant RBP function in plant immunity. Here, we describe the involvement of an RBP, Arabidopsis thaliana RNA-binding protein-defense related 1 (AtRBP-DR1; At4g03110), in resistance to the pathogen Pseudomonas syringae pv. tomato DC3000. AtRBP-DR1 loss-of-function mutants showed enhanced susceptibility to P. syringae pv. tomato DC3000. Overexpression of AtRBP-DR1 led to enhanced resistance to P. syringae pv. tomato DC3000 strains and dwarfism. The hypersensitive response triggered by P. syringae pv. tomato DC3000 avrRpt2 was compromised in the Atrbp-dr1 mutant and enhanced in the AtRBP-DR1 overexpression line at early time points. AtRBP-DR1 overexpression lines showed higher mRNA levels of SID2 and PR1, which are salicylic acid (SA) inducible, as well as spontaneous cell death in mature leaves. Consistent with these observations, the SA level was low in the Atrbp-dr1 mutant but high in the overexpression line. The SA-related phenotype in the overexpression line was fully dependent on SID2. Thus, AtRBP-DR1 is a positive regulator of SA-mediated immunity, possibly acting on SA signaling-related genes at a post-transcriptional level.

Authors+Show Affiliations

Department of Plant Biology, Microbial and Plant Genomics Institute, University of Minnesota, 1500 Gortner Avenue, St. Paul 55108, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

20636102

Citation

Qi, Yiping, et al. "A Putative RNA-binding Protein Positively Regulates Salicylic Acid-mediated Immunity in Arabidopsis." Molecular Plant-microbe Interactions : MPMI, vol. 23, no. 12, 2010, pp. 1573-83.
Qi Y, Tsuda K, Joe A, et al. A putative RNA-binding protein positively regulates salicylic acid-mediated immunity in Arabidopsis. Mol Plant Microbe Interact. 2010;23(12):1573-83.
Qi, Y., Tsuda, K., Joe, A., Sato, M., Nguyen, l. e. . V., Glazebrook, J., Alfano, J. R., Cohen, J. D., & Katagiri, F. (2010). A putative RNA-binding protein positively regulates salicylic acid-mediated immunity in Arabidopsis. Molecular Plant-microbe Interactions : MPMI, 23(12), 1573-83. https://doi.org/10.1094/MPMI-05-10-0106
Qi Y, et al. A Putative RNA-binding Protein Positively Regulates Salicylic Acid-mediated Immunity in Arabidopsis. Mol Plant Microbe Interact. 2010;23(12):1573-83. PubMed PMID: 20636102.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A putative RNA-binding protein positively regulates salicylic acid-mediated immunity in Arabidopsis. AU - Qi,Yiping, AU - Tsuda,Kenichi, AU - Joe,Anna, AU - Sato,Masanao, AU - Nguyen,Le V, AU - Glazebrook,Jane, AU - Alfano,James R, AU - Cohen,Jerry D, AU - Katagiri,Fumiaki, PY - 2010/7/20/entrez PY - 2010/7/20/pubmed PY - 2011/1/12/medline SP - 1573 EP - 83 JF - Molecular plant-microbe interactions : MPMI JO - Mol. Plant Microbe Interact. VL - 23 IS - 12 N2 - RNA-binding proteins (RBP) can control gene expression at both transcriptional and post-transcriptional levels. Plants respond to pathogen infection with rapid reprogramming of gene expression. However, little is known about how plant RBP function in plant immunity. Here, we describe the involvement of an RBP, Arabidopsis thaliana RNA-binding protein-defense related 1 (AtRBP-DR1; At4g03110), in resistance to the pathogen Pseudomonas syringae pv. tomato DC3000. AtRBP-DR1 loss-of-function mutants showed enhanced susceptibility to P. syringae pv. tomato DC3000. Overexpression of AtRBP-DR1 led to enhanced resistance to P. syringae pv. tomato DC3000 strains and dwarfism. The hypersensitive response triggered by P. syringae pv. tomato DC3000 avrRpt2 was compromised in the Atrbp-dr1 mutant and enhanced in the AtRBP-DR1 overexpression line at early time points. AtRBP-DR1 overexpression lines showed higher mRNA levels of SID2 and PR1, which are salicylic acid (SA) inducible, as well as spontaneous cell death in mature leaves. Consistent with these observations, the SA level was low in the Atrbp-dr1 mutant but high in the overexpression line. The SA-related phenotype in the overexpression line was fully dependent on SID2. Thus, AtRBP-DR1 is a positive regulator of SA-mediated immunity, possibly acting on SA signaling-related genes at a post-transcriptional level. SN - 0894-0282 UR - https://www.unboundmedicine.com/medline/citation/20636102/A_putative_RNA_binding_protein_positively_regulates_salicylic_acid_mediated_immunity_in_Arabidopsis_ L2 - http://apsjournals.apsnet.org/doi/full/10.1094/MPMI-05-10-0106?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -