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The Pseudomonas syringae effector protein HopZ1a suppresses effector-triggered immunity.
New Phytol. 2010 Sep; 187(4):1018-33.NP

Abstract

*The Pseudomonas syringae pv syringae type III effector HopZ1a is a member of the HopZ effector family of cysteine-proteases that triggers immunity in Arabidopsis. This immunity is dependent on HopZ1a cysteine-protease activity, and independent of known resistance genes. We have previously shown that HopZ1a-triggered immunity is partially additive to that triggered by AvrRpt2. These partially additive effects could be caused by at least two mechanisms: their signalling pathways share a common element(s), or one effector interferes with the response triggered by the other. *Here, we investigate the molecular basis for the partially additive effect displayed by AvrRpt2- and HopZ1a-triggered immunities, by analysing competitive indices, hypersensitive response and symptom induction, PR-1 accumulation, expression of PR genes, and systemic acquired resistance (SAR) induction. *Partially additive effects between these defence responses require HopZ1a cysteine-protease activity, and also take place between HopZ1a and AvrRps4 or AvrRpm1-triggered responses. We establish that HopZ1a-triggered immunity is independent of salicylic acid (SA), EDS1, jasmonic acid (JA) and ethylene (ET)-dependent pathways, and show that HopZ1a suppresses the induction of PR-1 and PR-5 associated with P. syringae pv tomato (Pto)-triggered effector-triggered immunity (ETI)-like defences, AvrRpt2-triggered immunity, and Pto or Pto (avrRpt2) activation of SAR, and that suppression requires HopZ1a cysteine-protease activity. *Our results indicate that HopZ1a triggers an unusual resistance independent of known pathways and suppresses SA and EDS1-dependent resistance.

Authors+Show Affiliations

Instituto de Hortofruticultura Subtropical y Mediterranea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Depto Biología Celular, Genética y Fisiología, Campus de Teatinos, Málaga E-29071, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20636323

Citation

Macho, Alberto P., et al. "The Pseudomonas Syringae Effector Protein HopZ1a Suppresses Effector-triggered Immunity." The New Phytologist, vol. 187, no. 4, 2010, pp. 1018-33.
Macho AP, Guevara CM, Tornero P, et al. The Pseudomonas syringae effector protein HopZ1a suppresses effector-triggered immunity. New Phytol. 2010;187(4):1018-33.
Macho, A. P., Guevara, C. M., Tornero, P., Ruiz-Albert, J., & Beuzón, C. R. (2010). The Pseudomonas syringae effector protein HopZ1a suppresses effector-triggered immunity. The New Phytologist, 187(4), 1018-33. https://doi.org/10.1111/j.1469-8137.2010.03381.x
Macho AP, et al. The Pseudomonas Syringae Effector Protein HopZ1a Suppresses Effector-triggered Immunity. New Phytol. 2010;187(4):1018-33. PubMed PMID: 20636323.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Pseudomonas syringae effector protein HopZ1a suppresses effector-triggered immunity. AU - Macho,Alberto P, AU - Guevara,Carlos M, AU - Tornero,Pablo, AU - Ruiz-Albert,Javier, AU - Beuzón,Carmen R, Y1 - 2010/07/15/ PY - 2010/7/20/entrez PY - 2010/7/20/pubmed PY - 2011/3/29/medline SP - 1018 EP - 33 JF - The New phytologist JO - New Phytol. VL - 187 IS - 4 N2 - *The Pseudomonas syringae pv syringae type III effector HopZ1a is a member of the HopZ effector family of cysteine-proteases that triggers immunity in Arabidopsis. This immunity is dependent on HopZ1a cysteine-protease activity, and independent of known resistance genes. We have previously shown that HopZ1a-triggered immunity is partially additive to that triggered by AvrRpt2. These partially additive effects could be caused by at least two mechanisms: their signalling pathways share a common element(s), or one effector interferes with the response triggered by the other. *Here, we investigate the molecular basis for the partially additive effect displayed by AvrRpt2- and HopZ1a-triggered immunities, by analysing competitive indices, hypersensitive response and symptom induction, PR-1 accumulation, expression of PR genes, and systemic acquired resistance (SAR) induction. *Partially additive effects between these defence responses require HopZ1a cysteine-protease activity, and also take place between HopZ1a and AvrRps4 or AvrRpm1-triggered responses. We establish that HopZ1a-triggered immunity is independent of salicylic acid (SA), EDS1, jasmonic acid (JA) and ethylene (ET)-dependent pathways, and show that HopZ1a suppresses the induction of PR-1 and PR-5 associated with P. syringae pv tomato (Pto)-triggered effector-triggered immunity (ETI)-like defences, AvrRpt2-triggered immunity, and Pto or Pto (avrRpt2) activation of SAR, and that suppression requires HopZ1a cysteine-protease activity. *Our results indicate that HopZ1a triggers an unusual resistance independent of known pathways and suppresses SA and EDS1-dependent resistance. SN - 1469-8137 UR - https://www.unboundmedicine.com/medline/citation/20636323/The_Pseudomonas_syringae_effector_protein_HopZ1a_suppresses_effector_triggered_immunity_ L2 - https://doi.org/10.1111/j.1469-8137.2010.03381.x DB - PRIME DP - Unbound Medicine ER -