TY - JOUR T1 - Determination of polycyclic aromatic hydrocarbons in food samples by automated on-line in-tube solid-phase microextraction coupled with high-performance liquid chromatography-fluorescence detection. AU - Ishizaki,A, AU - Saito,K, AU - Hanioka,N, AU - Narimatsu,S, AU - Kataoka,H, Y1 - 2010/06/30/ PY - 2010/05/02/received PY - 2010/06/15/revised PY - 2010/06/24/accepted PY - 2010/7/20/entrez PY - 2010/7/20/pubmed PY - 2010/11/10/medline SP - 5555 EP - 63 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1217 IS - 35 N2 - A simple and sensitive automated method, consisting of in-tube solid-phase microextraction (SPME) coupled with high-performance liquid chromatography-fluorescence detection (HPLC-FLD), was developed for the determination of 15 polycyclic aromatic hydrocarbons (PAHs) in food samples. PAHs were separated within 15 min by HPLC using a Zorbax Eclipse PAH column with a water/acetonitrile gradient elution program as the mobile phase. The optimum in-tube SPME conditions were 20 draw/eject cycles of 40 microL of sample using a CP-Sil 19CB capillary column as an extraction device. Low- and high-molecular weight PAHs were extracted effectively onto the capillary coating from 5% and 30% methanol solutions, respectively. The extracted PAHs were readily desorbed from the capillary by passage of the mobile phase, and no carryover was observed. Using the in-tube SPME HPLC-FLD method, good linearity of the calibration curve (r>0.9972) was obtained in the concentration range of 0.05-2.0 ng/mL, and the detection limits (S/N=3) of PAHs were 0.32-4.63 pg/mL. The in-tube SPME method showed 18-47 fold higher sensitivity than the direct injection method. The intra-day and inter-day precision (relative standard deviations) for a 1 ng/mL PAH mixture were below 5.1% and 7.6% (n=5), respectively. This method was applied successfully to the analysis of tea products and dried food samples without interference peaks, and the recoveries of PAHs spiked into the tea samples were >70%. Low-molecular weight PAHs such as naphthalene and pyrene were detected in many foods, and carcinogenic benzo[a]pyrene, at relatively high concentrations, was also detected in some black tea samples. This method was also utilized to assess the release of PAHs from tea leaves into the liquor. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/20637468/Determination_of_polycyclic_aromatic_hydrocarbons_in_food_samples_by_automated_on_line_in_tube_solid_phase_microextraction_coupled_with_high_performance_liquid_chromatography_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(10)00868-X DB - PRIME DP - Unbound Medicine ER -