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Life-cycle chronic gamma exposure of Arabidopsis thaliana induces growth effects but no discernable effects on oxidative stress pathways.
Plant Physiol Biochem. 2010 Sep; 48(9):778-86.PP

Abstract

Arabidopsis thaliana was exposed to low-dose chronic gamma irradiation during a full life cycle (seed to seed) and several biological responses were investigated. Applied dose rates were 2336, 367 and 81 microGy h(-1). Following 24 days (inflorescence emergence), 34 days (approximately 50% of flowers open) and 54 days (silice ripening) exposure, plants were harvested and monitored for biometric parameters, capacities of enzymes involved in the antioxidative defence mechanisms (SOD, APOD, GLUR, GPOD, SPOD, CAT, ME), glutathione and ascorbate pool, lipid peroxidation products, altered gene expression of selected genes encoding for antioxidative enzymes or reactive oxygen species production, and DNA integrity. Root fresh weight was significantly reduced after gamma exposure compared to the control at all stages monitored but no significant differences in root weight for the different dose rates applied was observed. Leaf and stem fresh weight were significantly reduced at the highest irradiation level after 54 days exposure only. Also total plant fresh was significantly lower at silice riping and this for the highest and medium dose rate applied. The dose rate estimated to result in a 10% reduction in growth (EDR-10) ranged between 60 and 80 microGy h(-1). Germination of seeds from the gamma irradiated plants was not hampered. For several of the antioxidative defence enzymes studied, the enzyme capacity was generally stimulated towards flowering but generally no significant effect of dose rate on enzyme capacity was observed. Gene analysis revealed a significant transient and dose dependent change in expression of RBOHC indicating active reactive oxygen production induced by gamma irradiation. No effect of irradiation was observed on concentration or reduction state of the non-enzymatic antioxidants, ascorbate and glutathione. The level of lipid peroxidation products remained constant throughout the observation period and was not affected by dose rate. The comet assay did not reveal any effect of gamma dose rate on DNA integrity.

Authors+Show Affiliations

Belgian Nuclear Research Centre (SCK.CEN), Biosphere Impact Studies, Boeretang 200, 2400 Mol, Belgium. hildegarde.vandenhove@sckcen.beNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20637647

Citation

Vandenhove, Hildegarde, et al. "Life-cycle Chronic Gamma Exposure of Arabidopsis Thaliana Induces Growth Effects but No Discernable Effects On Oxidative Stress Pathways." Plant Physiology and Biochemistry : PPB, vol. 48, no. 9, 2010, pp. 778-86.
Vandenhove H, Vanhoudt N, Cuypers A, et al. Life-cycle chronic gamma exposure of Arabidopsis thaliana induces growth effects but no discernable effects on oxidative stress pathways. Plant Physiol Biochem. 2010;48(9):778-86.
Vandenhove, H., Vanhoudt, N., Cuypers, A., van Hees, M., Wannijn, J., & Horemans, N. (2010). Life-cycle chronic gamma exposure of Arabidopsis thaliana induces growth effects but no discernable effects on oxidative stress pathways. Plant Physiology and Biochemistry : PPB, 48(9), 778-86. https://doi.org/10.1016/j.plaphy.2010.06.006
Vandenhove H, et al. Life-cycle Chronic Gamma Exposure of Arabidopsis Thaliana Induces Growth Effects but No Discernable Effects On Oxidative Stress Pathways. Plant Physiol Biochem. 2010;48(9):778-86. PubMed PMID: 20637647.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Life-cycle chronic gamma exposure of Arabidopsis thaliana induces growth effects but no discernable effects on oxidative stress pathways. AU - Vandenhove,Hildegarde, AU - Vanhoudt,Nathalie, AU - Cuypers,Ann, AU - van Hees,May, AU - Wannijn,Jean, AU - Horemans,Nele, Y1 - 2010/06/25/ PY - 2009/09/11/received PY - 2010/05/19/revised PY - 2010/06/18/accepted PY - 2010/7/20/entrez PY - 2010/7/20/pubmed PY - 2011/2/10/medline SP - 778 EP - 86 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol Biochem VL - 48 IS - 9 N2 - Arabidopsis thaliana was exposed to low-dose chronic gamma irradiation during a full life cycle (seed to seed) and several biological responses were investigated. Applied dose rates were 2336, 367 and 81 microGy h(-1). Following 24 days (inflorescence emergence), 34 days (approximately 50% of flowers open) and 54 days (silice ripening) exposure, plants were harvested and monitored for biometric parameters, capacities of enzymes involved in the antioxidative defence mechanisms (SOD, APOD, GLUR, GPOD, SPOD, CAT, ME), glutathione and ascorbate pool, lipid peroxidation products, altered gene expression of selected genes encoding for antioxidative enzymes or reactive oxygen species production, and DNA integrity. Root fresh weight was significantly reduced after gamma exposure compared to the control at all stages monitored but no significant differences in root weight for the different dose rates applied was observed. Leaf and stem fresh weight were significantly reduced at the highest irradiation level after 54 days exposure only. Also total plant fresh was significantly lower at silice riping and this for the highest and medium dose rate applied. The dose rate estimated to result in a 10% reduction in growth (EDR-10) ranged between 60 and 80 microGy h(-1). Germination of seeds from the gamma irradiated plants was not hampered. For several of the antioxidative defence enzymes studied, the enzyme capacity was generally stimulated towards flowering but generally no significant effect of dose rate on enzyme capacity was observed. Gene analysis revealed a significant transient and dose dependent change in expression of RBOHC indicating active reactive oxygen production induced by gamma irradiation. No effect of irradiation was observed on concentration or reduction state of the non-enzymatic antioxidants, ascorbate and glutathione. The level of lipid peroxidation products remained constant throughout the observation period and was not affected by dose rate. The comet assay did not reveal any effect of gamma dose rate on DNA integrity. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/20637647/Life_cycle_chronic_gamma_exposure_of_Arabidopsis_thaliana_induces_growth_effects_but_no_discernable_effects_on_oxidative_stress_pathways_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0981-9428(10)00136-1 DB - PRIME DP - Unbound Medicine ER -