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Assessment of prevalence and changing epidemiology of extended-spectrum beta-lactamase-producing Enterobacteriaceae fecal carriers using a chromogenic medium.
Diagn Microbiol Infect Dis. 2010 Aug; 67(4):376-9.DM

Abstract

Five hundred fecal samples from 462 patients (68.4% ambulatory) (February-April, 2007) from Madrid (Spain) were screened for extended-spectrum beta-lactamase (ESBL) producers using ceftazidime and cefotaxime (1 mg/L) MacConkey (MAC) agar plates and a chromogenic media (chromID ESBL; bioMérieux, Marcy-l'Etoile, France). bla(ESBL), qnr, aac(6')Ib-cr, and 16S rRNA methylase genes were assessed. A prevalence of 8.2% of ESBL fecal carriers was observed (8.9% hospitalized, 7.9% nonhospitalized patients), higher than that previously observed (1991, 0.6%; 2003, 7.0%). Sensitivity, specificity, and positive and negative predicted values were 100%, 94.8%, 63%, and 100% for chromID ESBL and 87.8%, 89.8%, 43.4%, and 98.9% for MAC, respectively. ESBL distribution was as follows: CTX-M-9-group, 40% (mainly CTX-M-14); CTX-M-1-group, 26.6% (mainly CTX-M-15); SHV-type, 29% (mainly SHV-12); and TEM-type, 4.4%. These enzymes were found in pulsed-field gel electrophoresis nonclonally related Escherichia coli and Klebsiella pneumoniae isolates. Transferable quinolone resistance was confirmed in CTX-M-9 (qnrS1), CTX-M-15 [aac(6')Ib-cr, qnrS1], and SHV-12 (qnrB7, qnrS1) producers but not 16S rRNA methylase genes. The chromID ESBL medium was reliable to screen ESBL fecal carriers with a general decrease in the laboratory workload. Time-to-time monitoring of ESBL fecal carriers is useful to ascertain current trend of ESBL epidemiology.

Authors+Show Affiliations

Servicio de Microbiología, Hospital Universitario Ramón y Cajal and CIBER en Epidemiología y Salud Pública (CIBERESP), Instituto de Investigación Ramón y Cajal (IRYCIS), 28034-Madrid, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20638607

Citation

Paniagua, Raquel, et al. "Assessment of Prevalence and Changing Epidemiology of Extended-spectrum Beta-lactamase-producing Enterobacteriaceae Fecal Carriers Using a Chromogenic Medium." Diagnostic Microbiology and Infectious Disease, vol. 67, no. 4, 2010, pp. 376-9.
Paniagua R, Valverde A, Coque TM, et al. Assessment of prevalence and changing epidemiology of extended-spectrum beta-lactamase-producing Enterobacteriaceae fecal carriers using a chromogenic medium. Diagn Microbiol Infect Dis. 2010;67(4):376-9.
Paniagua, R., Valverde, A., Coque, T. M., Baquero, F., & Cantón, R. (2010). Assessment of prevalence and changing epidemiology of extended-spectrum beta-lactamase-producing Enterobacteriaceae fecal carriers using a chromogenic medium. Diagnostic Microbiology and Infectious Disease, 67(4), 376-9. https://doi.org/10.1016/j.diagmicrobio.2010.03.012
Paniagua R, et al. Assessment of Prevalence and Changing Epidemiology of Extended-spectrum Beta-lactamase-producing Enterobacteriaceae Fecal Carriers Using a Chromogenic Medium. Diagn Microbiol Infect Dis. 2010;67(4):376-9. PubMed PMID: 20638607.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessment of prevalence and changing epidemiology of extended-spectrum beta-lactamase-producing Enterobacteriaceae fecal carriers using a chromogenic medium. AU - Paniagua,Raquel, AU - Valverde,Aránzazu, AU - Coque,Teresa M, AU - Baquero,Fernando, AU - Cantón,Rafael, PY - 2010/02/20/received PY - 2010/03/20/accepted PY - 2010/7/20/entrez PY - 2010/7/20/pubmed PY - 2010/10/20/medline SP - 376 EP - 9 JF - Diagnostic microbiology and infectious disease JO - Diagn. Microbiol. Infect. Dis. VL - 67 IS - 4 N2 - Five hundred fecal samples from 462 patients (68.4% ambulatory) (February-April, 2007) from Madrid (Spain) were screened for extended-spectrum beta-lactamase (ESBL) producers using ceftazidime and cefotaxime (1 mg/L) MacConkey (MAC) agar plates and a chromogenic media (chromID ESBL; bioMérieux, Marcy-l'Etoile, France). bla(ESBL), qnr, aac(6')Ib-cr, and 16S rRNA methylase genes were assessed. A prevalence of 8.2% of ESBL fecal carriers was observed (8.9% hospitalized, 7.9% nonhospitalized patients), higher than that previously observed (1991, 0.6%; 2003, 7.0%). Sensitivity, specificity, and positive and negative predicted values were 100%, 94.8%, 63%, and 100% for chromID ESBL and 87.8%, 89.8%, 43.4%, and 98.9% for MAC, respectively. ESBL distribution was as follows: CTX-M-9-group, 40% (mainly CTX-M-14); CTX-M-1-group, 26.6% (mainly CTX-M-15); SHV-type, 29% (mainly SHV-12); and TEM-type, 4.4%. These enzymes were found in pulsed-field gel electrophoresis nonclonally related Escherichia coli and Klebsiella pneumoniae isolates. Transferable quinolone resistance was confirmed in CTX-M-9 (qnrS1), CTX-M-15 [aac(6')Ib-cr, qnrS1], and SHV-12 (qnrB7, qnrS1) producers but not 16S rRNA methylase genes. The chromID ESBL medium was reliable to screen ESBL fecal carriers with a general decrease in the laboratory workload. Time-to-time monitoring of ESBL fecal carriers is useful to ascertain current trend of ESBL epidemiology. SN - 1879-0070 UR - https://www.unboundmedicine.com/medline/citation/20638607/Assessment_of_prevalence_and_changing_epidemiology_of_extended_spectrum_beta_lactamase_producing_Enterobacteriaceae_fecal_carriers_using_a_chromogenic_medium_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(10)00118-5 DB - PRIME DP - Unbound Medicine ER -