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Imbalance of NKp44(+)NKp46(-) and NKp44(-)NKp46(+) natural killer cells in the intestinal mucosa of patients with Crohn's disease.
Gastroenterology. 2010 Sep; 139(3):882-92, 892.e1-3.G

Abstract

BACKGROUND & AIMS

Mucosal natural killer (NK) cells that produce interleukin (IL)-22 mediate intestinal homeostasis and inflammation in mice. However, their role in the pathogenesis of human inflammatory bowel diseases (IBDs) is not known. We investigated intestinal NK cells in intestinal mucosa samples of patients with Crohn's disease (CD).

METHODS

We isolated lamina propria NK cells from intestinal mucosal samples of patients with IBD and subjects without IBD (controls) and analyzed expression patterns of cell surface molecules and cytokine production. Interactions between lamina propria NK cells and intestinal macrophages were examined.

RESULTS

In intestinal mucosa samples from controls, NKp44 and NKp46 were expressed differentially on CD3(-)CD56(+) NK cells, NKp44(+)NKp46(-) (NKp44(+)) NK cells expressed CD127 and the transcription factor retinoic acid-related orphan receptor C (RORC) and produced IL-22 whereas NKp44(-)NKp46(+) (NKp46(+)) NK cells did not express CD127 or RORC and produced interferon (IFN)-gamma. NKp46(+) NK cells were predominant in intestinal mucosa of patients with CD compared with controls or patients with ulcerative colitis. Upon interaction with intestinal inflammatory macrophages NKp46(+), NK cells from patients with CD were activated via IL-23 and produced IFN-gamma; this activation required cell-to-cell contact.

CONCLUSIONS

The balance of NKp44(+)/NKp46(+) NK cells is disrupted in intestinal mucosa of patients with CD. NKp46(+) NK cells might mediate the pathogenesis of CD by producing IFN-gamma.

Authors+Show Affiliations

Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20638936

Citation

Takayama, Tetsuro, et al. "Imbalance of NKp44(+)NKp46(-) and NKp44(-)NKp46(+) Natural Killer Cells in the Intestinal Mucosa of Patients With Crohn's Disease." Gastroenterology, vol. 139, no. 3, 2010, pp. 882-92, 892.e1-3.
Takayama T, Kamada N, Chinen H, et al. Imbalance of NKp44(+)NKp46(-) and NKp44(-)NKp46(+) natural killer cells in the intestinal mucosa of patients with Crohn's disease. Gastroenterology. 2010;139(3):882-92, 892.e1-3.
Takayama, T., Kamada, N., Chinen, H., Okamoto, S., Kitazume, M. T., Chang, J., Matuzaki, Y., Suzuki, S., Sugita, A., Koganei, K., Hisamatsu, T., Kanai, T., & Hibi, T. (2010). Imbalance of NKp44(+)NKp46(-) and NKp44(-)NKp46(+) natural killer cells in the intestinal mucosa of patients with Crohn's disease. Gastroenterology, 139(3), 882-92, e1-3. https://doi.org/10.1053/j.gastro.2010.05.040
Takayama T, et al. Imbalance of NKp44(+)NKp46(-) and NKp44(-)NKp46(+) Natural Killer Cells in the Intestinal Mucosa of Patients With Crohn's Disease. Gastroenterology. 2010;139(3):882-92, 892.e1-3. PubMed PMID: 20638936.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Imbalance of NKp44(+)NKp46(-) and NKp44(-)NKp46(+) natural killer cells in the intestinal mucosa of patients with Crohn's disease. AU - Takayama,Tetsuro, AU - Kamada,Nobuhiko, AU - Chinen,Hiroshi, AU - Okamoto,Susumu, AU - Kitazume,Mina T, AU - Chang,Jonathan, AU - Matuzaki,Yumi, AU - Suzuki,Sadafumi, AU - Sugita,Akira, AU - Koganei,Kazutaka, AU - Hisamatsu,Tadakazu, AU - Kanai,Takanori, AU - Hibi,Toshifumi, Y1 - 2010/06/01/ PY - 2009/11/23/received PY - 2010/04/30/revised PY - 2010/05/20/accepted PY - 2010/7/20/entrez PY - 2010/7/20/pubmed PY - 2010/9/21/medline SP - 882-92, 892.e1-3 JF - Gastroenterology JO - Gastroenterology VL - 139 IS - 3 N2 - BACKGROUND & AIMS: Mucosal natural killer (NK) cells that produce interleukin (IL)-22 mediate intestinal homeostasis and inflammation in mice. However, their role in the pathogenesis of human inflammatory bowel diseases (IBDs) is not known. We investigated intestinal NK cells in intestinal mucosa samples of patients with Crohn's disease (CD). METHODS: We isolated lamina propria NK cells from intestinal mucosal samples of patients with IBD and subjects without IBD (controls) and analyzed expression patterns of cell surface molecules and cytokine production. Interactions between lamina propria NK cells and intestinal macrophages were examined. RESULTS: In intestinal mucosa samples from controls, NKp44 and NKp46 were expressed differentially on CD3(-)CD56(+) NK cells, NKp44(+)NKp46(-) (NKp44(+)) NK cells expressed CD127 and the transcription factor retinoic acid-related orphan receptor C (RORC) and produced IL-22 whereas NKp44(-)NKp46(+) (NKp46(+)) NK cells did not express CD127 or RORC and produced interferon (IFN)-gamma. NKp46(+) NK cells were predominant in intestinal mucosa of patients with CD compared with controls or patients with ulcerative colitis. Upon interaction with intestinal inflammatory macrophages NKp46(+), NK cells from patients with CD were activated via IL-23 and produced IFN-gamma; this activation required cell-to-cell contact. CONCLUSIONS: The balance of NKp44(+)/NKp46(+) NK cells is disrupted in intestinal mucosa of patients with CD. NKp46(+) NK cells might mediate the pathogenesis of CD by producing IFN-gamma. SN - 1528-0012 UR - https://www.unboundmedicine.com/medline/citation/20638936/Imbalance_of_NKp44_+_NKp46____and_NKp44___NKp46_+__natural_killer_cells_in_the_intestinal_mucosa_of_patients_with_Crohn's_disease_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0016-5085(10)00801-2 DB - PRIME DP - Unbound Medicine ER -