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Multiple promoters contribute to swarming and the coordination of transcription with flagellar assembly in Salmonella.
J Bacteriol. 2010 Sep; 192(18):4752-62.JB

Abstract

In Salmonella, there are three classes of promoters in the flagellar transcriptional hierarchy. This organization allows genes needed earlier in the construction of flagella to be transcribed before genes needed later. Four operons (fliAZY, flgMN, fliDST, and flgKL) are expressed from both class 2 and class 3 promoters. To investigate the purpose for expressing genes from multiple flagellar promoters, mutants were constructed for each operon that were defective in either class 2 transcription or class 3 transcription. The mutants were checked for defects in swimming through liquids, swarming over surfaces, and transcriptional regulation. The expression of the hook-associated proteins (FlgK, FlgL, and FliD) from class 3 promoters was found to be important for swarming motility. Both flgMN promoters were involved in coordinating class 3 transcription with the stage of assembly of the hook-basal body. Finally, the fliAZY class 3 promoter lowered class 3 transcription in stationary phase. These results indicate that the multiple flagellar promoters respond to specific environmental conditions and help coordinate transcription with flagellar assembly.

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Authors+Show Affiliations

Wozniak CE
Department of Microbiology, University of Washington, Seattle, Washington 98195, USA. hughes@biology.utah.edu
Chevance FF
No affiliation info available
Hughes KT
No affiliation info available

MeSH

Bacterial ProteinsFlagellaMembrane ProteinsOperonPolymerase Chain ReactionPromoter Regions, GeneticSalmonella typhimuriumSigma FactorTranscription, Genetic

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

20639318

Citation

Wozniak, Christopher E., et al. "Multiple Promoters Contribute to Swarming and the Coordination of Transcription With Flagellar Assembly in Salmonella." Journal of Bacteriology, vol. 192, no. 18, 2010, pp. 4752-62.
Wozniak CE, Chevance FF, Hughes KT. Multiple promoters contribute to swarming and the coordination of transcription with flagellar assembly in Salmonella. J Bacteriol. 2010;192(18):4752-62.
Wozniak, C. E., Chevance, F. F., & Hughes, K. T. (2010). Multiple promoters contribute to swarming and the coordination of transcription with flagellar assembly in Salmonella. Journal of Bacteriology, 192(18), 4752-62. https://doi.org/10.1128/JB.00093-10
Wozniak CE, Chevance FF, Hughes KT. Multiple Promoters Contribute to Swarming and the Coordination of Transcription With Flagellar Assembly in Salmonella. J Bacteriol. 2010;192(18):4752-62. PubMed PMID: 20639318.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiple promoters contribute to swarming and the coordination of transcription with flagellar assembly in Salmonella. AU - Wozniak,Christopher E, AU - Chevance,Fabienne F V, AU - Hughes,Kelly T, Y1 - 2010/07/16/ PY - 2010/7/20/entrez PY - 2010/7/20/pubmed PY - 2010/9/14/medline SP - 4752 EP - 62 JF - Journal of bacteriology JO - J Bacteriol VL - 192 IS - 18 N2 - In Salmonella, there are three classes of promoters in the flagellar transcriptional hierarchy. This organization allows genes needed earlier in the construction of flagella to be transcribed before genes needed later. Four operons (fliAZY, flgMN, fliDST, and flgKL) are expressed from both class 2 and class 3 promoters. To investigate the purpose for expressing genes from multiple flagellar promoters, mutants were constructed for each operon that were defective in either class 2 transcription or class 3 transcription. The mutants were checked for defects in swimming through liquids, swarming over surfaces, and transcriptional regulation. The expression of the hook-associated proteins (FlgK, FlgL, and FliD) from class 3 promoters was found to be important for swarming motility. Both flgMN promoters were involved in coordinating class 3 transcription with the stage of assembly of the hook-basal body. Finally, the fliAZY class 3 promoter lowered class 3 transcription in stationary phase. These results indicate that the multiple flagellar promoters respond to specific environmental conditions and help coordinate transcription with flagellar assembly. SN - 1098-5530 UR - https://www.unboundmedicine.com/medline/citation/20639318/Multiple_promoters_contribute_to_swarming_and_the_coordination_of_transcription_with_flagellar_assembly_in_Salmonella_ L2 - https://journals.asm.org/doi/10.1128/JB.00093-10?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -