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Effects of a high-salt diet on TRPV-1-dependent renal nerve activity in Dahl salt-sensitive rats.
Am J Nephrol. 2010; 32(3):194-200.AJ

Abstract

OBJECTIVE

To test the hypothesis that transient receptor potential vanilloid type 1 channel (TRPV1)-mediated increases in afferent renal nerve activity (ARNA) and release of substance P (SP) and calcitonin gene-related peptide (CGRP) from the renal pelvis are suppressed in Dahl salt-sensitive (DS), but not -resistant (DR), rats fed a high-salt (HS) diet.

METHODS AND RESULTS

Male DS and DR rats were given a HS or low-salt (LS) diet for 3 weeks. Perfusion of capsaicin (CAP, 10(-6)M), a selective TRPV1 agonist, into the left renal pelvis increased ipsilateral ARNA in all groups, but with a smaller magnitude in DS-HS compared to other groups. CAP increased contralateral urine flow in all groups except DS-HS rats. CAP-induced release of SP and CGRP from the renal pelvis was less in DS-HS compared to other groups. Western blot showed that TRPV1 expression in the kidney decreased while expression of neurokinin 1 receptors increased in DS-HS compared to other groups.

CONCLUSION

TRPV1-mediated increases in ARNA and release of SP and CGRP in the renal pelvis are impaired in DS rats fed a HS diet, which can likely be attributed to suppressed TRPV1 expression in the kidney and contributes to increased salt sensitivity.

Authors+Show Affiliations

Department of Medicine, Michigan State University, East Lansing, Mich 48824, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20639627

Citation

Xie, Chaoqin, and Donna H. Wang. "Effects of a High-salt Diet On TRPV-1-dependent Renal Nerve Activity in Dahl Salt-sensitive Rats." American Journal of Nephrology, vol. 32, no. 3, 2010, pp. 194-200.
Xie C, Wang DH. Effects of a high-salt diet on TRPV-1-dependent renal nerve activity in Dahl salt-sensitive rats. Am J Nephrol. 2010;32(3):194-200.
Xie, C., & Wang, D. H. (2010). Effects of a high-salt diet on TRPV-1-dependent renal nerve activity in Dahl salt-sensitive rats. American Journal of Nephrology, 32(3), 194-200. https://doi.org/10.1159/000316528
Xie C, Wang DH. Effects of a High-salt Diet On TRPV-1-dependent Renal Nerve Activity in Dahl Salt-sensitive Rats. Am J Nephrol. 2010;32(3):194-200. PubMed PMID: 20639627.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of a high-salt diet on TRPV-1-dependent renal nerve activity in Dahl salt-sensitive rats. AU - Xie,Chaoqin, AU - Wang,Donna H, Y1 - 2010/07/15/ PY - 2010/05/05/received PY - 2010/06/02/accepted PY - 2010/7/20/entrez PY - 2010/7/20/pubmed PY - 2010/12/14/medline SP - 194 EP - 200 JF - American journal of nephrology JO - Am J Nephrol VL - 32 IS - 3 N2 - OBJECTIVE: To test the hypothesis that transient receptor potential vanilloid type 1 channel (TRPV1)-mediated increases in afferent renal nerve activity (ARNA) and release of substance P (SP) and calcitonin gene-related peptide (CGRP) from the renal pelvis are suppressed in Dahl salt-sensitive (DS), but not -resistant (DR), rats fed a high-salt (HS) diet. METHODS AND RESULTS: Male DS and DR rats were given a HS or low-salt (LS) diet for 3 weeks. Perfusion of capsaicin (CAP, 10(-6)M), a selective TRPV1 agonist, into the left renal pelvis increased ipsilateral ARNA in all groups, but with a smaller magnitude in DS-HS compared to other groups. CAP increased contralateral urine flow in all groups except DS-HS rats. CAP-induced release of SP and CGRP from the renal pelvis was less in DS-HS compared to other groups. Western blot showed that TRPV1 expression in the kidney decreased while expression of neurokinin 1 receptors increased in DS-HS compared to other groups. CONCLUSION: TRPV1-mediated increases in ARNA and release of SP and CGRP in the renal pelvis are impaired in DS rats fed a HS diet, which can likely be attributed to suppressed TRPV1 expression in the kidney and contributes to increased salt sensitivity. SN - 1421-9670 UR - https://www.unboundmedicine.com/medline/citation/20639627/Effects_of_a_high_salt_diet_on_TRPV_1_dependent_renal_nerve_activity_in_Dahl_salt_sensitive_rats_ L2 - https://www.karger.com?DOI=10.1159/000316528 DB - PRIME DP - Unbound Medicine ER -