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Mapping the myoglobin concentration, oxygenation, and optical pathlength in heart ex vivo using near-infrared imaging.
Anal Biochem 2010; 407(1):120-7AB

Abstract

A method that provides maps of absolute concentrations of oxygenated and deoxygenated myoglobin (Mb), its oxygenation, and its near-infrared (NIR) optical pathlength in cardiac tissue was developed. These parameters are available simultaneously. The method is based on NIR diffuse reflectance spectroscopic imaging and specific processing of the NIR images, which included a first derivative of the diffuse reflectance spectrum. Mb oxygenation, total Mb concentration, and NIR light pathlength were found to be in the range of 92%, 0.3 mM, and 12.5 mm, respectively, in beating isolated buffer-perfused and arrested pig hearts. The charge-coupled device camera enables sub-millimeter spatial resolution and spectroscopic imaging in 1.5 to 2.0 min. The technique is noninvasive and nondestructive. The equipment has no mechanical contact with the tissue of interest, leaving it undisturbed.

Authors+Show Affiliations

National Research Council of Canada Institute for Biodiagnostics, Winnipeg, Manitoba, Canada R3B 1Y6. eugene.gussakovsky@nrc-cnrc.gc.caNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20643093

Citation

Gussakovsky, Eugene, et al. "Mapping the Myoglobin Concentration, Oxygenation, and Optical Pathlength in Heart Ex Vivo Using Near-infrared Imaging." Analytical Biochemistry, vol. 407, no. 1, 2010, pp. 120-7.
Gussakovsky E, Yang Y, Rendell J, et al. Mapping the myoglobin concentration, oxygenation, and optical pathlength in heart ex vivo using near-infrared imaging. Anal Biochem. 2010;407(1):120-7.
Gussakovsky, E., Yang, Y., Rendell, J., Jilkina, O., & Kupriyanov, V. (2010). Mapping the myoglobin concentration, oxygenation, and optical pathlength in heart ex vivo using near-infrared imaging. Analytical Biochemistry, 407(1), pp. 120-7. doi:10.1016/j.ab.2010.07.010.
Gussakovsky E, et al. Mapping the Myoglobin Concentration, Oxygenation, and Optical Pathlength in Heart Ex Vivo Using Near-infrared Imaging. Anal Biochem. 2010 Dec 1;407(1):120-7. PubMed PMID: 20643093.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mapping the myoglobin concentration, oxygenation, and optical pathlength in heart ex vivo using near-infrared imaging. AU - Gussakovsky,Eugene, AU - Yang,Yanmin, AU - Rendell,John, AU - Jilkina,Olga, AU - Kupriyanov,Valery, Y1 - 2010/07/17/ PY - 2010/06/09/received PY - 2010/07/14/accepted PY - 2010/7/21/entrez PY - 2010/7/21/pubmed PY - 2011/1/19/medline SP - 120 EP - 7 JF - Analytical biochemistry JO - Anal. Biochem. VL - 407 IS - 1 N2 - A method that provides maps of absolute concentrations of oxygenated and deoxygenated myoglobin (Mb), its oxygenation, and its near-infrared (NIR) optical pathlength in cardiac tissue was developed. These parameters are available simultaneously. The method is based on NIR diffuse reflectance spectroscopic imaging and specific processing of the NIR images, which included a first derivative of the diffuse reflectance spectrum. Mb oxygenation, total Mb concentration, and NIR light pathlength were found to be in the range of 92%, 0.3 mM, and 12.5 mm, respectively, in beating isolated buffer-perfused and arrested pig hearts. The charge-coupled device camera enables sub-millimeter spatial resolution and spectroscopic imaging in 1.5 to 2.0 min. The technique is noninvasive and nondestructive. The equipment has no mechanical contact with the tissue of interest, leaving it undisturbed. SN - 1096-0309 UR - https://www.unboundmedicine.com/medline/citation/20643093/Mapping_the_myoglobin_concentration_oxygenation_and_optical_pathlength_in_heart_ex_vivo_using_near_infrared_imaging_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2697(10)00456-2 DB - PRIME DP - Unbound Medicine ER -