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Impact of D-pinitol on the attenuation of proinflammatory cytokines, hyperglycemia-mediated oxidative stress and protection of kidney tissue ultrastructure in streptozotocin-induced diabetic rats.
Chem Biol Interact. 2010 Oct 06; 188(1):237-45.CB

Abstract

Oxidative stress plays a crucial role in the progression and development of diabetes and its complications due to chronic hyperglycemia. The present study was aimed to investigate the kidney tissue protective nature of d-pinitol, a cyclitol present in soybean, by assessing the key markers of hyperglycemia-mediated oxidative stress, proinflammatory cytokines and ultrastructural alterations in streptozotocin-induced diabetic rats. Oral administration of d-pinitol (50mg/kg body weight/day) for 30 days to diabetic group of rats showed a significant elevation in the level of total protein and significant decline in the levels of blood urea, serum uric acid, creatinine and advanced glycation endproducts (AGEs) and kidney proinflammatory cytokines such as TNF-alpha, IL-1beta, IL-6, NF-kappaB p65 subunit and nitrite. Further, d-pinitol administration elicited a significant attenuation in the activities of kidney enzymatic antioxidants such as superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), glutathione-S-transferase (GST) and glutathione reductase (GR) and the levels of kidney non-enzymatic antioxidants such as vitamin E, vitamin C and reduced glutathione (GSH) in the diabetic group of rats, with a concomitant decline in the levels of kidney lipid peroxides, hydroperoxides and protein carbonyls. The histological and ultrastructural observations on the kidney tissues also confirmed the renoprotective nature of d-pinitol. Thus the present study demonstrated the renoprotective nature of d-pinitol by attenuating the hyperglycemia-mediated proinflammatory cytokines and antioxidant competence in kidney tissues of streptozotocin-induced diabetic rats.

Authors+Show Affiliations

Department of Biochemistry, University of Madras, Guindy Campus, Chennai 600025, Tamil Nadu, India.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20643114

Citation

Sivakumar, Selvaraj, et al. "Impact of D-pinitol On the Attenuation of Proinflammatory Cytokines, Hyperglycemia-mediated Oxidative Stress and Protection of Kidney Tissue Ultrastructure in Streptozotocin-induced Diabetic Rats." Chemico-biological Interactions, vol. 188, no. 1, 2010, pp. 237-45.
Sivakumar S, Palsamy P, Subramanian SP. Impact of D-pinitol on the attenuation of proinflammatory cytokines, hyperglycemia-mediated oxidative stress and protection of kidney tissue ultrastructure in streptozotocin-induced diabetic rats. Chem Biol Interact. 2010;188(1):237-45.
Sivakumar, S., Palsamy, P., & Subramanian, S. P. (2010). Impact of D-pinitol on the attenuation of proinflammatory cytokines, hyperglycemia-mediated oxidative stress and protection of kidney tissue ultrastructure in streptozotocin-induced diabetic rats. Chemico-biological Interactions, 188(1), 237-45. https://doi.org/10.1016/j.cbi.2010.07.014
Sivakumar S, Palsamy P, Subramanian SP. Impact of D-pinitol On the Attenuation of Proinflammatory Cytokines, Hyperglycemia-mediated Oxidative Stress and Protection of Kidney Tissue Ultrastructure in Streptozotocin-induced Diabetic Rats. Chem Biol Interact. 2010 Oct 6;188(1):237-45. PubMed PMID: 20643114.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impact of D-pinitol on the attenuation of proinflammatory cytokines, hyperglycemia-mediated oxidative stress and protection of kidney tissue ultrastructure in streptozotocin-induced diabetic rats. AU - Sivakumar,Selvaraj, AU - Palsamy,Periyasamy, AU - Subramanian,Sorimuthu Pillai, Y1 - 2010/07/17/ PY - 2010/05/15/received PY - 2010/07/02/revised PY - 2010/07/12/accepted PY - 2010/7/21/entrez PY - 2010/7/21/pubmed PY - 2010/10/5/medline SP - 237 EP - 45 JF - Chemico-biological interactions JO - Chem Biol Interact VL - 188 IS - 1 N2 - Oxidative stress plays a crucial role in the progression and development of diabetes and its complications due to chronic hyperglycemia. The present study was aimed to investigate the kidney tissue protective nature of d-pinitol, a cyclitol present in soybean, by assessing the key markers of hyperglycemia-mediated oxidative stress, proinflammatory cytokines and ultrastructural alterations in streptozotocin-induced diabetic rats. Oral administration of d-pinitol (50mg/kg body weight/day) for 30 days to diabetic group of rats showed a significant elevation in the level of total protein and significant decline in the levels of blood urea, serum uric acid, creatinine and advanced glycation endproducts (AGEs) and kidney proinflammatory cytokines such as TNF-alpha, IL-1beta, IL-6, NF-kappaB p65 subunit and nitrite. Further, d-pinitol administration elicited a significant attenuation in the activities of kidney enzymatic antioxidants such as superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), glutathione-S-transferase (GST) and glutathione reductase (GR) and the levels of kidney non-enzymatic antioxidants such as vitamin E, vitamin C and reduced glutathione (GSH) in the diabetic group of rats, with a concomitant decline in the levels of kidney lipid peroxides, hydroperoxides and protein carbonyls. The histological and ultrastructural observations on the kidney tissues also confirmed the renoprotective nature of d-pinitol. Thus the present study demonstrated the renoprotective nature of d-pinitol by attenuating the hyperglycemia-mediated proinflammatory cytokines and antioxidant competence in kidney tissues of streptozotocin-induced diabetic rats. SN - 1872-7786 UR - https://www.unboundmedicine.com/medline/citation/20643114/Impact_of_D_pinitol_on_the_attenuation_of_proinflammatory_cytokines_hyperglycemia_mediated_oxidative_stress_and_protection_of_kidney_tissue_ultrastructure_in_streptozotocin_induced_diabetic_rats_ DB - PRIME DP - Unbound Medicine ER -