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Comparison of an in-house latex agglutination test with IgM ELISA and MAT in the diagnosis of leptospirosis.
Indian J Med Microbiol. 2010 Jul-Sep; 28(3):238-40.IJ

Abstract

The laboratory diagnosis of leptospirosis is fraught with several problems. Isolation of Leptospira by culture has a low sensitivity and the microscopic agglutination test (MAT) is time consuming To overcome these problems, a rapid latex agglutination test (LAT) has been standardized for the detection of antileptospiral antibodies in serum samples from suspected cases of leptospirosis. We compared the efficiency of the LAT to a commercially available IgM ELISA and MAT. A total of 150 serum samples were tested by LAT, IgM ELISA, and MAT. The positivity was 26.7%, 26% and 24% respectively. The sensitivity and specificity of LAT as compared to MAT was 90.62 and 91.96% respectively. Even though LAT and ELISA showed similar results, its rapidity and simplicity made latex agglutination test more suitable as a rapid screening test.

Authors+Show Affiliations

Department of Microbiology, JIPMER, Pondicherry, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

20644313

Citation

Shekatkar, S, et al. "Comparison of an In-house Latex Agglutination Test With IgM ELISA and MAT in the Diagnosis of Leptospirosis." Indian Journal of Medical Microbiology, vol. 28, no. 3, 2010, pp. 238-40.
Shekatkar S, Acharya NS, Harish BN, et al. Comparison of an in-house latex agglutination test with IgM ELISA and MAT in the diagnosis of leptospirosis. Indian J Med Microbiol. 2010;28(3):238-40.
Shekatkar, S., Acharya, N. S., Harish, B. N., & Parija, S. C. (2010). Comparison of an in-house latex agglutination test with IgM ELISA and MAT in the diagnosis of leptospirosis. Indian Journal of Medical Microbiology, 28(3), 238-40. https://doi.org/10.4103/0255-0857.66484
Shekatkar S, et al. Comparison of an In-house Latex Agglutination Test With IgM ELISA and MAT in the Diagnosis of Leptospirosis. Indian J Med Microbiol. 2010 Jul-Sep;28(3):238-40. PubMed PMID: 20644313.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of an in-house latex agglutination test with IgM ELISA and MAT in the diagnosis of leptospirosis. AU - Shekatkar,S, AU - Acharya,N S, AU - Harish,B N, AU - Parija,S C, PY - 2010/7/21/entrez PY - 2010/7/21/pubmed PY - 2010/10/20/medline SP - 238 EP - 40 JF - Indian journal of medical microbiology JO - Indian J Med Microbiol VL - 28 IS - 3 N2 - The laboratory diagnosis of leptospirosis is fraught with several problems. Isolation of Leptospira by culture has a low sensitivity and the microscopic agglutination test (MAT) is time consuming To overcome these problems, a rapid latex agglutination test (LAT) has been standardized for the detection of antileptospiral antibodies in serum samples from suspected cases of leptospirosis. We compared the efficiency of the LAT to a commercially available IgM ELISA and MAT. A total of 150 serum samples were tested by LAT, IgM ELISA, and MAT. The positivity was 26.7%, 26% and 24% respectively. The sensitivity and specificity of LAT as compared to MAT was 90.62 and 91.96% respectively. Even though LAT and ELISA showed similar results, its rapidity and simplicity made latex agglutination test more suitable as a rapid screening test. SN - 1998-3646 UR - https://www.unboundmedicine.com/medline/citation/20644313/Comparison_of_an_in_house_latex_agglutination_test_with_IgM_ELISA_and_MAT_in_the_diagnosis_of_leptospirosis_ L2 - http://www.ijmm.org/article.asp?issn=0255-0857;year=2010;volume=28;issue=3;spage=238;epage=240;aulast=Shekatkar DB - PRIME DP - Unbound Medicine ER -