Tags

Type your tag names separated by a space and hit enter

Enhanced production of silymarin by Ag+ elicitor in cell suspension cultures of Silybum marianum.
Pharm Biol 2010; 48(6):708-15PB

Abstract

Cell suspension cultures of Silybum marianum L. Gaertn (Compositae) produce silymarin, a mixture of flavonolignans. In an attempt to increase cell growth and silymarin production, we exposed cell cultures to various levels of Ag+ (0.2, 0.4, 0.8, 1, and 2 mM) for different exposure times (12, 24, 48, 72, 144, and 216 h). A dramatic increase in cell growth was observed after 12 h in media supplemented with 0.2, 0.4, 0.8, and 1 mM Ag+, and the value in medium treated by 1 mM Ag+ after 72 h was 7.21 g, which was about two-fold that of the control (3.32 g). The highest silymarin production reached about 56 microg g(-1) DW, 24 h after treatment with Ag+ (0.8 mM), which was 30-fold that of the control. Silybin, isosilybin, silychristin, silydianin, and taxifolin were abundant flavonolignans. Ag+ in low concentrations is a positive elicitor for cell growth and silymarin production in cell suspension cultures of Silybum marianum.

Authors+Show Affiliations

Department of Plant Physiology and Proteomics, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20645746

Citation

Ashtiani, S Rahimi, et al. "Enhanced Production of Silymarin By Ag+ Elicitor in Cell Suspension Cultures of Silybum Marianum." Pharmaceutical Biology, vol. 48, no. 6, 2010, pp. 708-15.
Ashtiani SR, Hasanloo T, Bihamta MR. Enhanced production of silymarin by Ag+ elicitor in cell suspension cultures of Silybum marianum. Pharm Biol. 2010;48(6):708-15.
Ashtiani, S. R., Hasanloo, T., & Bihamta, M. R. (2010). Enhanced production of silymarin by Ag+ elicitor in cell suspension cultures of Silybum marianum. Pharmaceutical Biology, 48(6), pp. 708-15. doi:10.3109/13880200903264426.
Ashtiani SR, Hasanloo T, Bihamta MR. Enhanced Production of Silymarin By Ag+ Elicitor in Cell Suspension Cultures of Silybum Marianum. Pharm Biol. 2010;48(6):708-15. PubMed PMID: 20645746.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhanced production of silymarin by Ag+ elicitor in cell suspension cultures of Silybum marianum. AU - Ashtiani,S Rahimi, AU - Hasanloo,T, AU - Bihamta,M R, PY - 2010/7/22/entrez PY - 2010/7/22/pubmed PY - 2010/10/28/medline SP - 708 EP - 15 JF - Pharmaceutical biology JO - Pharm Biol VL - 48 IS - 6 N2 - Cell suspension cultures of Silybum marianum L. Gaertn (Compositae) produce silymarin, a mixture of flavonolignans. In an attempt to increase cell growth and silymarin production, we exposed cell cultures to various levels of Ag+ (0.2, 0.4, 0.8, 1, and 2 mM) for different exposure times (12, 24, 48, 72, 144, and 216 h). A dramatic increase in cell growth was observed after 12 h in media supplemented with 0.2, 0.4, 0.8, and 1 mM Ag+, and the value in medium treated by 1 mM Ag+ after 72 h was 7.21 g, which was about two-fold that of the control (3.32 g). The highest silymarin production reached about 56 microg g(-1) DW, 24 h after treatment with Ag+ (0.8 mM), which was 30-fold that of the control. Silybin, isosilybin, silychristin, silydianin, and taxifolin were abundant flavonolignans. Ag+ in low concentrations is a positive elicitor for cell growth and silymarin production in cell suspension cultures of Silybum marianum. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/20645746/Enhanced_production_of_silymarin_by_Ag+_elicitor_in_cell_suspension_cultures_of_Silybum_marianum_ L2 - http://www.tandfonline.com/doi/full/10.3109/13880200903264426 DB - PRIME DP - Unbound Medicine ER -