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Antioxidant, free radical-scavenging activity and cytotoxicity of different solvent extracts and their phenolic constituents from the fruit hull of mangosteen (Garcinia mangostana).
Pharm Biol. 2010 Jan; 48(1):55-62.PB

Abstract

Antioxidative, skin protective activities, and cytotoxicity of three extracts (water, methanol, and hexane) from the fruit hull of mangosteen (Garcinia mangostana Linn. (Guttiferae)) and their phenolic constituents such as alpha-mangostin, epicatechin, and tannin, were evaluated. The amounts of alpha-mangostin, total flavonoid, and total tannin were different among the three extracts, except those of total tannin in methanol and hexane extracts. For the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free-radical scavenging, hydroxyl radical-scavenging, and inhibition of lipid peroxidation experiment, the water extract showed higher activity than the methanol extract and hexane extract. alpha-Mangostin, epicatechin, and tannin also revealed these antioxidant and free radical-scavenging activities. When added simultaneously with H(2)O(2) (200 microM) to keratinocyte cells, the water extract (50 microg/mL), epicatechin (200 microM), and tannin (200 microM) effectively protected cells from oxidative damage, but the methanol extract, hexane extract, and alpha-mangostin did not. The methanol extract and hexane extract exhibited moderate cytotoxicity, whereas alpha-mangostin showed strong cytotoxicity. The present study provides the evidence that Garcinia mangostana extracts, especially the G. mangostana water extract, act as antioxidants and cytoprotective agents against oxidative damage, which is at least partly due to its phenolic compounds in mangosteen.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Ngawhirunpat T
Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, Thailand.
Opanasopi P
No affiliation info available
Sukma M
No affiliation info available
Sittisombut C
No affiliation info available
Kat A
No affiliation info available
Adachi I
No affiliation info available

MeSH

AnimalsAntioxidantsCells, CulturedCytotoxinsFree Radical ScavengersFruitGarcinia mangostanaHumansKeratinocytesMaleMiceMice, Inbred BALB CMiddle AgedPhenolsPlant ExtractsSolventsXanthones

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20645756

Citation

Ngawhirunpat, Tanasait, et al. "Antioxidant, Free Radical-scavenging Activity and Cytotoxicity of Different Solvent Extracts and Their Phenolic Constituents From the Fruit Hull of Mangosteen (Garcinia Mangostana)." Pharmaceutical Biology, vol. 48, no. 1, 2010, pp. 55-62.
Ngawhirunpat T, Opanasopi P, Sukma M, et al. Antioxidant, free radical-scavenging activity and cytotoxicity of different solvent extracts and their phenolic constituents from the fruit hull of mangosteen (Garcinia mangostana). Pharm Biol. 2010;48(1):55-62.
Ngawhirunpat, T., Opanasopi, P., Sukma, M., Sittisombut, C., Kat, A., & Adachi, I. (2010). Antioxidant, free radical-scavenging activity and cytotoxicity of different solvent extracts and their phenolic constituents from the fruit hull of mangosteen (Garcinia mangostana). Pharmaceutical Biology, 48(1), 55-62. https://doi.org/10.3109/13880200903046138
Ngawhirunpat T, et al. Antioxidant, Free Radical-scavenging Activity and Cytotoxicity of Different Solvent Extracts and Their Phenolic Constituents From the Fruit Hull of Mangosteen (Garcinia Mangostana). Pharm Biol. 2010;48(1):55-62. PubMed PMID: 20645756.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant, free radical-scavenging activity and cytotoxicity of different solvent extracts and their phenolic constituents from the fruit hull of mangosteen (Garcinia mangostana). AU - Ngawhirunpat,Tanasait, AU - Opanasopi,Praneet, AU - Sukma,Monrudee, AU - Sittisombut,Chavalit, AU - Kat,Atsushi, AU - Adachi,Isao, PY - 2010/7/22/entrez PY - 2010/7/22/pubmed PY - 2011/4/26/medline SP - 55 EP - 62 JF - Pharmaceutical biology JO - Pharm Biol VL - 48 IS - 1 N2 - Antioxidative, skin protective activities, and cytotoxicity of three extracts (water, methanol, and hexane) from the fruit hull of mangosteen (Garcinia mangostana Linn. (Guttiferae)) and their phenolic constituents such as alpha-mangostin, epicatechin, and tannin, were evaluated. The amounts of alpha-mangostin, total flavonoid, and total tannin were different among the three extracts, except those of total tannin in methanol and hexane extracts. For the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free-radical scavenging, hydroxyl radical-scavenging, and inhibition of lipid peroxidation experiment, the water extract showed higher activity than the methanol extract and hexane extract. alpha-Mangostin, epicatechin, and tannin also revealed these antioxidant and free radical-scavenging activities. When added simultaneously with H(2)O(2) (200 microM) to keratinocyte cells, the water extract (50 microg/mL), epicatechin (200 microM), and tannin (200 microM) effectively protected cells from oxidative damage, but the methanol extract, hexane extract, and alpha-mangostin did not. The methanol extract and hexane extract exhibited moderate cytotoxicity, whereas alpha-mangostin showed strong cytotoxicity. The present study provides the evidence that Garcinia mangostana extracts, especially the G. mangostana water extract, act as antioxidants and cytoprotective agents against oxidative damage, which is at least partly due to its phenolic compounds in mangosteen. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/20645756/Antioxidant_free_radical_scavenging_activity_and_cytotoxicity_of_different_solvent_extracts_and_their_phenolic_constituents_from_the_fruit_hull_of_mangosteen__Garcinia_mangostana__ DB - PRIME DP - Unbound Medicine ER -