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Temperature alters ethanol-induced fluidization of C57 mouse brain membranes.
Alcohol. 1991 Mar-Apr; 8(2):117-21.A

Abstract

The interaction between temperature and ethanol-induced fluidization was investigated in brain synaptic plasma membranes from C57BL/6 mice. Changes in fluidity were measured using the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene. Fluorescence polarization was tested in the presence and absence of ethanol at 25, 32 and 37 degrees C. An increase in temperature resulted in a significant increase in the baseline fluidity of the membranes and an increase in the magnitude of ethanol-induced fluidization of brain membranes. The combined effect of temperature on baseline fluidity and the magnitude of the response to ethanol resulted in a significant temperature-related increase in the relative response to ethanol (% change in polarization). The minimum concentration of ethanol required to cause a significant increase in the fluidity of the membranes was 170.7 mM at 25 degrees C and 85.3 mM at both 32 and 37 degrees C. The present results indicate that temperature-related changes in the effects of ethanol on membrane properties may underlie the effects of temperature on ethanol sensitivity in C57 mice.

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Authors+Show Affiliations

Bejanian M
Alcohol and Brain Research Laboratory, School of Pharmacy, University of Southern California, Los Angeles 90033.
Alkana RL
No affiliation info available
von Hungen K
No affiliation info available
Baxter CF
No affiliation info available
Syapin PJ
No affiliation info available

MeSH

Analysis of VarianceAnimalsBody TemperatureBrainDiphenylhexatrieneEthanolFluorescence PolarizationIn Vitro TechniquesMaleMembrane FluidityMiceMice, Inbred C57BLRegression AnalysisSynaptic Membranes

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2064752

Citation

Bejanian, M, et al. "Temperature Alters Ethanol-induced Fluidization of C57 Mouse Brain Membranes." Alcohol (Fayetteville, N.Y.), vol. 8, no. 2, 1991, pp. 117-21.
Bejanian M, Alkana RL, von Hungen K, et al. Temperature alters ethanol-induced fluidization of C57 mouse brain membranes. Alcohol. 1991;8(2):117-21.
Bejanian, M., Alkana, R. L., von Hungen, K., Baxter, C. F., & Syapin, P. J. (1991). Temperature alters ethanol-induced fluidization of C57 mouse brain membranes. Alcohol (Fayetteville, N.Y.), 8(2), 117-21.
Bejanian M, et al. Temperature Alters Ethanol-induced Fluidization of C57 Mouse Brain Membranes. Alcohol. 1991 Mar-Apr;8(2):117-21. PubMed PMID: 2064752.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Temperature alters ethanol-induced fluidization of C57 mouse brain membranes. AU - Bejanian,M, AU - Alkana,R L, AU - von Hungen,K, AU - Baxter,C F, AU - Syapin,P J, PY - 1991/3/1/pubmed PY - 1991/3/1/medline PY - 1991/3/1/entrez SP - 117 EP - 21 JF - Alcohol (Fayetteville, N.Y.) JO - Alcohol VL - 8 IS - 2 N2 - The interaction between temperature and ethanol-induced fluidization was investigated in brain synaptic plasma membranes from C57BL/6 mice. Changes in fluidity were measured using the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene. Fluorescence polarization was tested in the presence and absence of ethanol at 25, 32 and 37 degrees C. An increase in temperature resulted in a significant increase in the baseline fluidity of the membranes and an increase in the magnitude of ethanol-induced fluidization of brain membranes. The combined effect of temperature on baseline fluidity and the magnitude of the response to ethanol resulted in a significant temperature-related increase in the relative response to ethanol (% change in polarization). The minimum concentration of ethanol required to cause a significant increase in the fluidity of the membranes was 170.7 mM at 25 degrees C and 85.3 mM at both 32 and 37 degrees C. The present results indicate that temperature-related changes in the effects of ethanol on membrane properties may underlie the effects of temperature on ethanol sensitivity in C57 mice. SN - 0741-8329 UR - https://www.unboundmedicine.com/medline/citation/2064752/Temperature_alters_ethanol_induced_fluidization_of_C57_mouse_brain_membranes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0741-8329(91)91319-W DB - PRIME DP - Unbound Medicine ER -