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Temperature alters ethanol-induced fluidization of C57 mouse brain membranes.
Alcohol. 1991 Mar-Apr; 8(2):117-21.A

Abstract

The interaction between temperature and ethanol-induced fluidization was investigated in brain synaptic plasma membranes from C57BL/6 mice. Changes in fluidity were measured using the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene. Fluorescence polarization was tested in the presence and absence of ethanol at 25, 32 and 37 degrees C. An increase in temperature resulted in a significant increase in the baseline fluidity of the membranes and an increase in the magnitude of ethanol-induced fluidization of brain membranes. The combined effect of temperature on baseline fluidity and the magnitude of the response to ethanol resulted in a significant temperature-related increase in the relative response to ethanol (% change in polarization). The minimum concentration of ethanol required to cause a significant increase in the fluidity of the membranes was 170.7 mM at 25 degrees C and 85.3 mM at both 32 and 37 degrees C. The present results indicate that temperature-related changes in the effects of ethanol on membrane properties may underlie the effects of temperature on ethanol sensitivity in C57 mice.

Authors+Show Affiliations

Alcohol and Brain Research Laboratory, School of Pharmacy, University of Southern California, Los Angeles 90033.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2064752

Citation

Bejanian, M, et al. "Temperature Alters Ethanol-induced Fluidization of C57 Mouse Brain Membranes." Alcohol (Fayetteville, N.Y.), vol. 8, no. 2, 1991, pp. 117-21.
Bejanian M, Alkana RL, von Hungen K, et al. Temperature alters ethanol-induced fluidization of C57 mouse brain membranes. Alcohol. 1991;8(2):117-21.
Bejanian, M., Alkana, R. L., von Hungen, K., Baxter, C. F., & Syapin, P. J. (1991). Temperature alters ethanol-induced fluidization of C57 mouse brain membranes. Alcohol (Fayetteville, N.Y.), 8(2), 117-21.
Bejanian M, et al. Temperature Alters Ethanol-induced Fluidization of C57 Mouse Brain Membranes. Alcohol. 1991 Mar-Apr;8(2):117-21. PubMed PMID: 2064752.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Temperature alters ethanol-induced fluidization of C57 mouse brain membranes. AU - Bejanian,M, AU - Alkana,R L, AU - von Hungen,K, AU - Baxter,C F, AU - Syapin,P J, PY - 1991/3/1/pubmed PY - 1991/3/1/medline PY - 1991/3/1/entrez SP - 117 EP - 21 JF - Alcohol (Fayetteville, N.Y.) JO - Alcohol VL - 8 IS - 2 N2 - The interaction between temperature and ethanol-induced fluidization was investigated in brain synaptic plasma membranes from C57BL/6 mice. Changes in fluidity were measured using the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene. Fluorescence polarization was tested in the presence and absence of ethanol at 25, 32 and 37 degrees C. An increase in temperature resulted in a significant increase in the baseline fluidity of the membranes and an increase in the magnitude of ethanol-induced fluidization of brain membranes. The combined effect of temperature on baseline fluidity and the magnitude of the response to ethanol resulted in a significant temperature-related increase in the relative response to ethanol (% change in polarization). The minimum concentration of ethanol required to cause a significant increase in the fluidity of the membranes was 170.7 mM at 25 degrees C and 85.3 mM at both 32 and 37 degrees C. The present results indicate that temperature-related changes in the effects of ethanol on membrane properties may underlie the effects of temperature on ethanol sensitivity in C57 mice. SN - 0741-8329 UR - https://www.unboundmedicine.com/medline/citation/2064752/Temperature_alters_ethanol_induced_fluidization_of_C57_mouse_brain_membranes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0741-8329(91)91319-W DB - PRIME DP - Unbound Medicine ER -