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Nanomaterial-amplified "signal off/on" electrogenerated chemiluminescence aptasensors for the detection of thrombin.
Biosens Bioelectron. 2010 Oct 15; 26(2):754-9.BB

Abstract

Two electrogenerated chemiluminescence (ECL) aptasensors for the detection of thrombin were developed using the thrombin binding aptamer (TBA) taken as a molecular recognition element and nanomaterial as a carrier of the ECL capture/signal probe. In the "signal off" aptasensor, the thiolated capture probe (ss-DNA, 12-mer) was self-assembled on the gold nanoparticles (GNPs) which were self-assembled on the surface of gold electrode, and hybridized with six-base segment of the ss-DNA sequence (Tgt-aptamer, 21-mer) containing TBA-I (ss-DNA, 15-mer) tagged with ruthenium complex, producing a high ECL intensity. Introduction of the analyte thrombin triggered the dissociation of the Tgt-aptamer tagged with ruthenium complex from the aptasensors, led to significantly decrease in ECL intensity. The decreased ECL intensity was in proportion to the concentration of thrombin in a range from 2.7×10(-12) to 2.7×10(-9) M with a detection limit of 8×10(-13) M. In the "signal on" aptasensor, the thiolated TBA-I was self-assembled on the gold electrode for capturing thrombin onto the electrode and then the TBA-II (ss-DNA, 29-mer) labeled with single-walled carbon-nanotubes (SWNT)-ECL tag was bound with epitope of thrombin, producing a high ECL intensity. The increased ECL intensity was linearly with the concentration of thrombin from 1.0×10(-14) M to 1.0×10(-11) M with a detection limit of 3×10(-15) M. The present work demonstrates that using nanomaterial as a carrier for capture probe and signal probe is a promising way to amplify the ECL signal and to improve the sensitivity of the aptasensors.

Authors+Show Affiliations

Key Laboratory of Applied Surface and Colloid Chemistry (Shaanxi Normal University), Ministry of Education, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20650626

Citation

Li, Yan, et al. "Nanomaterial-amplified "signal Off/on" Electrogenerated Chemiluminescence Aptasensors for the Detection of Thrombin." Biosensors & Bioelectronics, vol. 26, no. 2, 2010, pp. 754-9.
Li Y, Qi H, Gao Q, et al. Nanomaterial-amplified "signal off/on" electrogenerated chemiluminescence aptasensors for the detection of thrombin. Biosens Bioelectron. 2010;26(2):754-9.
Li, Y., Qi, H., Gao, Q., Yang, J., & Zhang, C. (2010). Nanomaterial-amplified "signal off/on" electrogenerated chemiluminescence aptasensors for the detection of thrombin. Biosensors & Bioelectronics, 26(2), 754-9. https://doi.org/10.1016/j.bios.2010.06.044
Li Y, et al. Nanomaterial-amplified "signal Off/on" Electrogenerated Chemiluminescence Aptasensors for the Detection of Thrombin. Biosens Bioelectron. 2010 Oct 15;26(2):754-9. PubMed PMID: 20650626.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nanomaterial-amplified "signal off/on" electrogenerated chemiluminescence aptasensors for the detection of thrombin. AU - Li,Yan, AU - Qi,Honglan, AU - Gao,Qiang, AU - Yang,Jia, AU - Zhang,Chengxiao, Y1 - 2010/07/03/ PY - 2010/04/06/received PY - 2010/06/10/revised PY - 2010/06/25/accepted PY - 2010/7/24/entrez PY - 2010/7/24/pubmed PY - 2011/2/8/medline SP - 754 EP - 9 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 26 IS - 2 N2 - Two electrogenerated chemiluminescence (ECL) aptasensors for the detection of thrombin were developed using the thrombin binding aptamer (TBA) taken as a molecular recognition element and nanomaterial as a carrier of the ECL capture/signal probe. In the "signal off" aptasensor, the thiolated capture probe (ss-DNA, 12-mer) was self-assembled on the gold nanoparticles (GNPs) which were self-assembled on the surface of gold electrode, and hybridized with six-base segment of the ss-DNA sequence (Tgt-aptamer, 21-mer) containing TBA-I (ss-DNA, 15-mer) tagged with ruthenium complex, producing a high ECL intensity. Introduction of the analyte thrombin triggered the dissociation of the Tgt-aptamer tagged with ruthenium complex from the aptasensors, led to significantly decrease in ECL intensity. The decreased ECL intensity was in proportion to the concentration of thrombin in a range from 2.7×10(-12) to 2.7×10(-9) M with a detection limit of 8×10(-13) M. In the "signal on" aptasensor, the thiolated TBA-I was self-assembled on the gold electrode for capturing thrombin onto the electrode and then the TBA-II (ss-DNA, 29-mer) labeled with single-walled carbon-nanotubes (SWNT)-ECL tag was bound with epitope of thrombin, producing a high ECL intensity. The increased ECL intensity was linearly with the concentration of thrombin from 1.0×10(-14) M to 1.0×10(-11) M with a detection limit of 3×10(-15) M. The present work demonstrates that using nanomaterial as a carrier for capture probe and signal probe is a promising way to amplify the ECL signal and to improve the sensitivity of the aptasensors. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/20650626/Nanomaterial_amplified_"signal_off/on"_electrogenerated_chemiluminescence_aptasensors_for_the_detection_of_thrombin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(10)00345-3 DB - PRIME DP - Unbound Medicine ER -