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[Ginsenoside Rg1 protects rat hippocampal neurons from radiation injury by regulating NOS activity].
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Jul; 30(7):1522-5.NF

Abstract

OBJECTIVE

To study the protective effect of ginsenoside Rg1 on cultured rat hippocampal neurons against radiation injury and explore new therapies for preventing radiation encephalopathy.

METHODS

Rat hippocampal neurons cultured for 12 days were subjected to a single-dose X-ray exposure of 30 Gy. 4',6-diamidino-2-phenylindole (DAPI) staining was used to detect the neuronal apoptosis and NOS activity kit utilized to evaluate NOS activity in the cells after the exposure.

RESULTS

Nuclear condensation was detected in (25.3-/+3.57)% of the neurons 24 h after 30 Gy X-ray exposure, a rate significantly higher than that in the control cells [(1.95-/+0.78)%, P<0.01]. In the neurons pretreated with ginsenoside Rg1, only (7.43-/+1.51)% of the cells presented with nuclear condensation after the exposure, significantly different from the rates in the control cultures and the exposed cultures (P<0.01). The NOS activity of exposed cultures was 6.46-/+0.95 U/ml, significantly higher than that in the control cultures (3.20-/+0.70 U/ml, P<0.01). The NOS activity of the neurons pretreated with ginsenoside Rg1 was 3.85-/+0.69 U/ml, significantly different from that in the control cultures (P<0.05) and the exposed cultures (P<0.01).

CONCLUSION

Ginsenoside Rg1 offers significant protective effect on rat hippocampal neurons from radiation-induced apoptosis by reducing the activity of NOS.

Authors+Show Affiliations

Department of Radiotherapy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. poor9927@126.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

chi

PubMed ID

20650756

Citation

Chen, Yong, et al. "[Ginsenoside Rg1 Protects Rat Hippocampal Neurons From Radiation Injury By Regulating NOS Activity]." Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, vol. 30, no. 7, 2010, pp. 1522-5.
Chen Y, Sun AM, Chen ZX, et al. [Ginsenoside Rg1 protects rat hippocampal neurons from radiation injury by regulating NOS activity]. Nan Fang Yi Ke Da Xue Xue Bao. 2010;30(7):1522-5.
Chen, Y., Sun, A. M., Chen, Z. X., Liu, Y., Chen, L. H., & Yuan, Y. W. (2010). [Ginsenoside Rg1 protects rat hippocampal neurons from radiation injury by regulating NOS activity]. Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, 30(7), 1522-5.
Chen Y, et al. [Ginsenoside Rg1 Protects Rat Hippocampal Neurons From Radiation Injury By Regulating NOS Activity]. Nan Fang Yi Ke Da Xue Xue Bao. 2010;30(7):1522-5. PubMed PMID: 20650756.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Ginsenoside Rg1 protects rat hippocampal neurons from radiation injury by regulating NOS activity]. AU - Chen,Yong, AU - Sun,Ai-min, AU - Chen,Zhi-xian, AU - Liu,Ying, AU - Chen,Long-hua, AU - Yuan,Ya-wei, PY - 2010/7/24/entrez PY - 2010/7/24/pubmed PY - 2013/6/26/medline SP - 1522 EP - 5 JF - Nan fang yi ke da xue xue bao = Journal of Southern Medical University JO - Nan Fang Yi Ke Da Xue Xue Bao VL - 30 IS - 7 N2 - OBJECTIVE: To study the protective effect of ginsenoside Rg1 on cultured rat hippocampal neurons against radiation injury and explore new therapies for preventing radiation encephalopathy. METHODS: Rat hippocampal neurons cultured for 12 days were subjected to a single-dose X-ray exposure of 30 Gy. 4',6-diamidino-2-phenylindole (DAPI) staining was used to detect the neuronal apoptosis and NOS activity kit utilized to evaluate NOS activity in the cells after the exposure. RESULTS: Nuclear condensation was detected in (25.3-/+3.57)% of the neurons 24 h after 30 Gy X-ray exposure, a rate significantly higher than that in the control cells [(1.95-/+0.78)%, P<0.01]. In the neurons pretreated with ginsenoside Rg1, only (7.43-/+1.51)% of the cells presented with nuclear condensation after the exposure, significantly different from the rates in the control cultures and the exposed cultures (P<0.01). The NOS activity of exposed cultures was 6.46-/+0.95 U/ml, significantly higher than that in the control cultures (3.20-/+0.70 U/ml, P<0.01). The NOS activity of the neurons pretreated with ginsenoside Rg1 was 3.85-/+0.69 U/ml, significantly different from that in the control cultures (P<0.05) and the exposed cultures (P<0.01). CONCLUSION: Ginsenoside Rg1 offers significant protective effect on rat hippocampal neurons from radiation-induced apoptosis by reducing the activity of NOS. SN - 1673-4254 UR - https://www.unboundmedicine.com/medline/citation/20650756/[Ginsenoside_Rg1_protects_rat_hippocampal_neurons_from_radiation_injury_by_regulating_NOS_activity]_ DB - PRIME DP - Unbound Medicine ER -