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Comparing diagnostic accuracy of Kato-Katz, Koga agar plate, ether-concentration, and FLOTAC for Schistosoma mansoni and soil-transmitted helminths.
PLoS Negl Trop Dis. 2010 Jul 20; 4(7):e754.PN

Abstract

BACKGROUND

Infections with schistosomes and soil-transmitted helminths exert a considerable yet underappreciated economic and public health burden on afflicted populations. Accurate diagnosis is crucial for patient management, drug efficacy evaluations, and monitoring of large-scale community-based control programs.

METHODS/PRINCIPAL FINDINGS

The diagnostic accuracy of four copromicroscopic techniques (i.e., Kato-Katz, Koga agar plate, ether-concentration, and FLOTAC) for the detection of Schistosoma mansoni and soil-transmitted helminth eggs was compared using stool samples from 112 school children in Côte d'Ivoire. Combined results of all four methods served as a diagnostic 'gold' standard and revealed prevalences of S. mansoni, hookworm, Trichuris trichiura, Strongyloides stercoralis and Ascaris lumbricoides of 83.0%, 55.4%, 40.2%, 33.9% and 28.6%, respectively. A single FLOTAC from stool samples preserved in sodium acetate-acetic acid-formalin for 30 or 83 days showed a higher sensitivity for S. mansoni diagnosis (91.4%) than the ether-concentration method on stool samples preserved for 40 days (85.0%) or triplicate Kato-Katz using fresh stool samples (77.4%). Moreover, a single FLOTAC detected hookworm, A. lumbricoides and T. trichiura infections with a higher sensitivity than any of the other methods used, but resulted in lower egg counts. The Koga agar plate method was the most accurate diagnostic assay for S. stercoralis.

CONCLUSION/SIGNIFICANCE

We have shown that the FLOTAC method holds promise for the diagnosis of S. mansoni. Moreover, our study confirms that FLOTAC is a sensitive technique for detection of common soil-transmitted helminths. For the diagnosis of S. stercoralis, the Koga agar plate method remains the method of choice.

Authors+Show Affiliations

Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20651931

Citation

Glinz, Dominik, et al. "Comparing Diagnostic Accuracy of Kato-Katz, Koga Agar Plate, Ether-concentration, and FLOTAC for Schistosoma Mansoni and Soil-transmitted Helminths." PLoS Neglected Tropical Diseases, vol. 4, no. 7, 2010, pp. e754.
Glinz D, Silué KD, Knopp S, et al. Comparing diagnostic accuracy of Kato-Katz, Koga agar plate, ether-concentration, and FLOTAC for Schistosoma mansoni and soil-transmitted helminths. PLoS Negl Trop Dis. 2010;4(7):e754.
Glinz, D., Silué, K. D., Knopp, S., Lohourignon, L. K., Yao, K. P., Steinmann, P., Rinaldi, L., Cringoli, G., N'Goran, E. K., & Utzinger, J. (2010). Comparing diagnostic accuracy of Kato-Katz, Koga agar plate, ether-concentration, and FLOTAC for Schistosoma mansoni and soil-transmitted helminths. PLoS Neglected Tropical Diseases, 4(7), e754. https://doi.org/10.1371/journal.pntd.0000754
Glinz D, et al. Comparing Diagnostic Accuracy of Kato-Katz, Koga Agar Plate, Ether-concentration, and FLOTAC for Schistosoma Mansoni and Soil-transmitted Helminths. PLoS Negl Trop Dis. 2010 Jul 20;4(7):e754. PubMed PMID: 20651931.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparing diagnostic accuracy of Kato-Katz, Koga agar plate, ether-concentration, and FLOTAC for Schistosoma mansoni and soil-transmitted helminths. AU - Glinz,Dominik, AU - Silué,Kigbafori D, AU - Knopp,Stefanie, AU - Lohourignon,Laurent K, AU - Yao,Kouassi P, AU - Steinmann,Peter, AU - Rinaldi,Laura, AU - Cringoli,Giuseppe, AU - N'Goran,Eliézer K, AU - Utzinger,Jürg, Y1 - 2010/07/20/ PY - 2009/12/11/received PY - 2010/06/04/accepted PY - 2010/7/24/entrez PY - 2010/7/24/pubmed PY - 2010/10/15/medline SP - e754 EP - e754 JF - PLoS neglected tropical diseases JO - PLoS Negl Trop Dis VL - 4 IS - 7 N2 - BACKGROUND: Infections with schistosomes and soil-transmitted helminths exert a considerable yet underappreciated economic and public health burden on afflicted populations. Accurate diagnosis is crucial for patient management, drug efficacy evaluations, and monitoring of large-scale community-based control programs. METHODS/PRINCIPAL FINDINGS: The diagnostic accuracy of four copromicroscopic techniques (i.e., Kato-Katz, Koga agar plate, ether-concentration, and FLOTAC) for the detection of Schistosoma mansoni and soil-transmitted helminth eggs was compared using stool samples from 112 school children in Côte d'Ivoire. Combined results of all four methods served as a diagnostic 'gold' standard and revealed prevalences of S. mansoni, hookworm, Trichuris trichiura, Strongyloides stercoralis and Ascaris lumbricoides of 83.0%, 55.4%, 40.2%, 33.9% and 28.6%, respectively. A single FLOTAC from stool samples preserved in sodium acetate-acetic acid-formalin for 30 or 83 days showed a higher sensitivity for S. mansoni diagnosis (91.4%) than the ether-concentration method on stool samples preserved for 40 days (85.0%) or triplicate Kato-Katz using fresh stool samples (77.4%). Moreover, a single FLOTAC detected hookworm, A. lumbricoides and T. trichiura infections with a higher sensitivity than any of the other methods used, but resulted in lower egg counts. The Koga agar plate method was the most accurate diagnostic assay for S. stercoralis. CONCLUSION/SIGNIFICANCE: We have shown that the FLOTAC method holds promise for the diagnosis of S. mansoni. Moreover, our study confirms that FLOTAC is a sensitive technique for detection of common soil-transmitted helminths. For the diagnosis of S. stercoralis, the Koga agar plate method remains the method of choice. SN - 1935-2735 UR - https://www.unboundmedicine.com/medline/citation/20651931/Comparing_diagnostic_accuracy_of_Kato_Katz_Koga_agar_plate_ether_concentration_and_FLOTAC_for_Schistosoma_mansoni_and_soil_transmitted_helminths_ L2 - https://dx.plos.org/10.1371/journal.pntd.0000754 DB - PRIME DP - Unbound Medicine ER -