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Easy genotyping of complement C3 'slow' and 'fast' allotypes by tetra-primer amplification refractory mutation system PCR.

Abstract

Complement C3 'slow' and 'fast' allotypes are associated with immune-mediated disorders and may affect the outcome of renal transplantation. We report a tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) that provides a rapid, reproducible and cost-effective method to genotype both complement C3 'slow' and 'fast' alleles by a single tube reaction.

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  • Authors

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    Source

    Molecular and cellular probes 24:6 2010 Dec pg 401-2

    MeSH

    Alleles
    Cell Line
    Complement C3
    DNA Primers
    Genotype
    Humans
    Mutation
    Polymerase Chain Reaction
    Polymorphism, Single Nucleotide

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    20655380