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Easy genotyping of complement C3 'slow' and 'fast' allotypes by tetra-primer amplification refractory mutation system PCR.
Complement C3 'slow' and 'fast' allotypes are associated with immune-mediated disorders and may affect the outcome of renal transplantation. We report a tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) that provides a rapid, reproducible and cost-effective method to genotype both complement C3 'slow' and 'fast' alleles by a single tube reaction.
Authors, , , , , , , , , ,
Polymerase Chain Reaction
Polymorphism, Single Nucleotide
Pub Type(s)Journal Article
Research Support, Non-U.S. Gov't