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Broiler chickens, broiler chicken meat, pigs and pork as sources of ExPEC related virulence genes and resistance in Escherichia coli isolates from community-dwelling humans and UTI patients.
Int J Food Microbiol. 2010 Aug 15; 142(1-2):264-72.IJ

Abstract

Urinary tract infection (UTI) is one of the most common bacterial infections. UTI is primarily caused by extraintestinal pathogenic Escherichia coli (ExPEC) from the patients' own fecal flora. The ExPEC often belong to phylogroups B2 and D, the groups which include potent human ExPEC isolates causing UTI, bacteremia, and meningitis. The external sources of these ExPEC in the human intestine are unknown. The food supply may transmit ExPEC to humans. However, evidence of this hypothesis is limited. To assess this hypothesis, the objective of our study was to investigate the presence of ExPEC related virulence genes in E. coli isolates from UTI patients, community-dwelling humans, meat, and production animals. Accordingly, we included 964 geographically and temporally matched E. coli isolates from UTI patients (n=102), community-dwelling humans (n=109), fresh Danish (n=197) and imported broiler chicken meat (n=86), broiler chickens (n=138), fresh Danish (n=177) and imported pork (n=10), and pigs (n=145) in the study. All isolates were investigated for the presence of eight ExPEC related genes (kpsM II, papA, papC, iutA, sfaS, focG, afa, hlyD) using PCR. To investigate any similarities between isolates from the different origins, we performed a cluster analysis including antimicrobial resistance data previously published. We detected seven of the eight ExPEC related genes in isolates from broiler chicken meat, broiler chickens, pork and pigs. Our findings suggest that broiler chicken meat, broiler chickens, pork and pigs could be the source of strains with these ExPEC related virulence genes in community-dwelling humans and UTI patients. Especially detection of ExPEC related virulence genes in isolates belonging to phylogroups B2 and D is very concerning and may have a significant medical impact. The cluster analysis of virulence gene and antimicrobial resistance profiles showed strong similarities between UTI patient, community-dwelling human isolates, meat, and production animal isolates. Thus, these strains from meat and production animals may pose a zoonotic risk.

Authors+Show Affiliations

National Center for Antimicrobials & Infection Control, Statens Serum Institut, Copenhagen S, Denmark. lja@ssi.dkNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20656368

Citation

Jakobsen, Lotte, et al. "Broiler Chickens, Broiler Chicken Meat, Pigs and Pork as Sources of ExPEC Related Virulence Genes and Resistance in Escherichia Coli Isolates From Community-dwelling Humans and UTI Patients." International Journal of Food Microbiology, vol. 142, no. 1-2, 2010, pp. 264-72.
Jakobsen L, Spangholm DJ, Pedersen K, et al. Broiler chickens, broiler chicken meat, pigs and pork as sources of ExPEC related virulence genes and resistance in Escherichia coli isolates from community-dwelling humans and UTI patients. Int J Food Microbiol. 2010;142(1-2):264-72.
Jakobsen, L., Spangholm, D. J., Pedersen, K., Jensen, L. B., Emborg, H. D., Agersø, Y., Aarestrup, F. M., Hammerum, A. M., & Frimodt-Møller, N. (2010). Broiler chickens, broiler chicken meat, pigs and pork as sources of ExPEC related virulence genes and resistance in Escherichia coli isolates from community-dwelling humans and UTI patients. International Journal of Food Microbiology, 142(1-2), 264-72. https://doi.org/10.1016/j.ijfoodmicro.2010.06.025
Jakobsen L, et al. Broiler Chickens, Broiler Chicken Meat, Pigs and Pork as Sources of ExPEC Related Virulence Genes and Resistance in Escherichia Coli Isolates From Community-dwelling Humans and UTI Patients. Int J Food Microbiol. 2010 Aug 15;142(1-2):264-72. PubMed PMID: 20656368.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Broiler chickens, broiler chicken meat, pigs and pork as sources of ExPEC related virulence genes and resistance in Escherichia coli isolates from community-dwelling humans and UTI patients. AU - Jakobsen,Lotte, AU - Spangholm,Daniel J, AU - Pedersen,Karl, AU - Jensen,Lars B, AU - Emborg,Hanne-Dorthe, AU - Agersø,Yvonne, AU - Aarestrup,Frank M, AU - Hammerum,Anette M, AU - Frimodt-Møller,Niels, Y1 - 2010/07/03/ PY - 2009/11/17/received PY - 2010/06/02/revised PY - 2010/06/27/accepted PY - 2010/7/27/entrez PY - 2010/7/27/pubmed PY - 2010/12/14/medline SP - 264 EP - 72 JF - International journal of food microbiology JO - Int J Food Microbiol VL - 142 IS - 1-2 N2 - Urinary tract infection (UTI) is one of the most common bacterial infections. UTI is primarily caused by extraintestinal pathogenic Escherichia coli (ExPEC) from the patients' own fecal flora. The ExPEC often belong to phylogroups B2 and D, the groups which include potent human ExPEC isolates causing UTI, bacteremia, and meningitis. The external sources of these ExPEC in the human intestine are unknown. The food supply may transmit ExPEC to humans. However, evidence of this hypothesis is limited. To assess this hypothesis, the objective of our study was to investigate the presence of ExPEC related virulence genes in E. coli isolates from UTI patients, community-dwelling humans, meat, and production animals. Accordingly, we included 964 geographically and temporally matched E. coli isolates from UTI patients (n=102), community-dwelling humans (n=109), fresh Danish (n=197) and imported broiler chicken meat (n=86), broiler chickens (n=138), fresh Danish (n=177) and imported pork (n=10), and pigs (n=145) in the study. All isolates were investigated for the presence of eight ExPEC related genes (kpsM II, papA, papC, iutA, sfaS, focG, afa, hlyD) using PCR. To investigate any similarities between isolates from the different origins, we performed a cluster analysis including antimicrobial resistance data previously published. We detected seven of the eight ExPEC related genes in isolates from broiler chicken meat, broiler chickens, pork and pigs. Our findings suggest that broiler chicken meat, broiler chickens, pork and pigs could be the source of strains with these ExPEC related virulence genes in community-dwelling humans and UTI patients. Especially detection of ExPEC related virulence genes in isolates belonging to phylogroups B2 and D is very concerning and may have a significant medical impact. The cluster analysis of virulence gene and antimicrobial resistance profiles showed strong similarities between UTI patient, community-dwelling human isolates, meat, and production animal isolates. Thus, these strains from meat and production animals may pose a zoonotic risk. SN - 1879-3460 UR - https://www.unboundmedicine.com/medline/citation/20656368/Broiler_chickens_broiler_chicken_meat_pigs_and_pork_as_sources_of_ExPEC_related_virulence_genes_and_resistance_in_Escherichia_coli_isolates_from_community_dwelling_humans_and_UTI_patients_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1605(10)00367-3 DB - PRIME DP - Unbound Medicine ER -