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Complete nucleotide sequence of the IncN plasmid pKOX105 encoding VIM-1, QnrS1 and SHV-12 proteins in Enterobacteriaceae from Bolzano, Italy compared with IncN plasmids encoding KPC enzymes in the USA.
J Antimicrob Chemother. 2010 Oct; 65(10):2070-5.JA

Abstract

OBJECTIVES

We determined the complete nucleotide sequence of pKOX105, a 54 641 bp plasmid from a Klebsiella oxytoca strain that was isolated from a resident of a long-term-care facility in Bolzano, Italy.

METHODS

The plasmid was sequenced using a shotgun approach. Combinatorial PCRs, directed PCRs and walking reads were used to assemble the contigs and to fill in gaps. Gene sequences were compared with reference plasmids and aligned with GenBank data using BLAST and CLUSTAL W software.

RESULTS

pKOX105 belonged to incompatibility group IncN, harboured bla(VIM-1), bla(SHV-12), qnrS1, aacA4 and dfrA14 and conferred resistance to carbapenems, oxyimino-cephalosporins, quinolones, aminoglycosides and trimethoprim. It was highly related to the p9 and p12 plasmids from Klebsiella pneumoniae and K. oxytoca strains isolated at a New York City hospital in 2005 carrying bla(KPC-2) and bla(KPC-3), respectively.

CONCLUSIONS

IncN plasmids are broad host-range plasmids that have contributed significantly to the worldwide dissemination of many different resistance genes in Enterobacteriaceae from animal and human sources. This plasmid family is now playing a crucial role in the global spread of diverse carbapenemase genes in Klebsiella spp.

Authors+Show Affiliations

Department of Infectious, Parasitic and Immune-mediated Diseases, Istituto Superiore di Sanita, Rome, Italy. alecara@iss.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20656680

Citation

Carattoli, Alessandra, et al. "Complete Nucleotide Sequence of the IncN Plasmid pKOX105 Encoding VIM-1, QnrS1 and SHV-12 Proteins in Enterobacteriaceae From Bolzano, Italy Compared With IncN Plasmids Encoding KPC Enzymes in the USA." The Journal of Antimicrobial Chemotherapy, vol. 65, no. 10, 2010, pp. 2070-5.
Carattoli A, Aschbacher R, March A, et al. Complete nucleotide sequence of the IncN plasmid pKOX105 encoding VIM-1, QnrS1 and SHV-12 proteins in Enterobacteriaceae from Bolzano, Italy compared with IncN plasmids encoding KPC enzymes in the USA. J Antimicrob Chemother. 2010;65(10):2070-5.
Carattoli, A., Aschbacher, R., March, A., Larcher, C., Livermore, D. M., & Woodford, N. (2010). Complete nucleotide sequence of the IncN plasmid pKOX105 encoding VIM-1, QnrS1 and SHV-12 proteins in Enterobacteriaceae from Bolzano, Italy compared with IncN plasmids encoding KPC enzymes in the USA. The Journal of Antimicrobial Chemotherapy, 65(10), 2070-5. https://doi.org/10.1093/jac/dkq269
Carattoli A, et al. Complete Nucleotide Sequence of the IncN Plasmid pKOX105 Encoding VIM-1, QnrS1 and SHV-12 Proteins in Enterobacteriaceae From Bolzano, Italy Compared With IncN Plasmids Encoding KPC Enzymes in the USA. J Antimicrob Chemother. 2010;65(10):2070-5. PubMed PMID: 20656680.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Complete nucleotide sequence of the IncN plasmid pKOX105 encoding VIM-1, QnrS1 and SHV-12 proteins in Enterobacteriaceae from Bolzano, Italy compared with IncN plasmids encoding KPC enzymes in the USA. AU - Carattoli,Alessandra, AU - Aschbacher,Richard, AU - March,Albert, AU - Larcher,Clara, AU - Livermore,David M, AU - Woodford,Neil, Y1 - 2010/07/22/ PY - 2010/7/27/entrez PY - 2010/7/27/pubmed PY - 2011/1/22/medline SP - 2070 EP - 5 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 65 IS - 10 N2 - OBJECTIVES: We determined the complete nucleotide sequence of pKOX105, a 54 641 bp plasmid from a Klebsiella oxytoca strain that was isolated from a resident of a long-term-care facility in Bolzano, Italy. METHODS: The plasmid was sequenced using a shotgun approach. Combinatorial PCRs, directed PCRs and walking reads were used to assemble the contigs and to fill in gaps. Gene sequences were compared with reference plasmids and aligned with GenBank data using BLAST and CLUSTAL W software. RESULTS: pKOX105 belonged to incompatibility group IncN, harboured bla(VIM-1), bla(SHV-12), qnrS1, aacA4 and dfrA14 and conferred resistance to carbapenems, oxyimino-cephalosporins, quinolones, aminoglycosides and trimethoprim. It was highly related to the p9 and p12 plasmids from Klebsiella pneumoniae and K. oxytoca strains isolated at a New York City hospital in 2005 carrying bla(KPC-2) and bla(KPC-3), respectively. CONCLUSIONS: IncN plasmids are broad host-range plasmids that have contributed significantly to the worldwide dissemination of many different resistance genes in Enterobacteriaceae from animal and human sources. This plasmid family is now playing a crucial role in the global spread of diverse carbapenemase genes in Klebsiella spp. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/20656680/Complete_nucleotide_sequence_of_the_IncN_plasmid_pKOX105_encoding_VIM_1_QnrS1_and_SHV_12_proteins_in_Enterobacteriaceae_from_Bolzano_Italy_compared_with_IncN_plasmids_encoding_KPC_enzymes_in_the_USA_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkq269 DB - PRIME DP - Unbound Medicine ER -