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Red fluorescent protein with reversibly photoswitchable absorbance for photochromic FRET.
Chem Biol 2010; 17(7):745-55CB

Abstract

We have developed the first red fluorescent protein, named rsTagRFP, which possesses reversibly photoswitchable absorbance spectra. Illumination with blue and yellow light switches rsTagRFP into a red fluorescent state (ON state) or nonfluorescent state (OFF state), respectively. The ON and OFF states exhibit absorbance maxima at 567 and 440 nm, respectively. Due to the photoswitchable absorbance, rsTagRFP can be used as an acceptor for a photochromic Förster resonance energy transfer (pcFRET). The photochromic acceptor facilitates determination of a protein-protein interaction by providing an internal control for FRET. Using pcFRET with EYFP as a donor, we observed an interaction between epidermal growth factor receptor and growth factor receptor-binding protein 2 in live cells by detecting the modulation of both the fluorescence intensity and lifetime of the EYFP donor upon the ON-OFF photoswitching of the rsTagRFP acceptor.

Authors+Show Affiliations

Department of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20659687

Citation

Subach, Fedor V., et al. "Red Fluorescent Protein With Reversibly Photoswitchable Absorbance for Photochromic FRET." Chemistry & Biology, vol. 17, no. 7, 2010, pp. 745-55.
Subach FV, Zhang L, Gadella TW, et al. Red fluorescent protein with reversibly photoswitchable absorbance for photochromic FRET. Chem Biol. 2010;17(7):745-55.
Subach, F. V., Zhang, L., Gadella, T. W., Gurskaya, N. G., Lukyanov, K. A., & Verkhusha, V. V. (2010). Red fluorescent protein with reversibly photoswitchable absorbance for photochromic FRET. Chemistry & Biology, 17(7), pp. 745-55. doi:10.1016/j.chembiol.2010.05.022.
Subach FV, et al. Red Fluorescent Protein With Reversibly Photoswitchable Absorbance for Photochromic FRET. Chem Biol. 2010 Jul 30;17(7):745-55. PubMed PMID: 20659687.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Red fluorescent protein with reversibly photoswitchable absorbance for photochromic FRET. AU - Subach,Fedor V, AU - Zhang,Lijuan, AU - Gadella,Theodorus W J, AU - Gurskaya,Nadya G, AU - Lukyanov,Konstantin A, AU - Verkhusha,Vladislav V, PY - 2009/12/31/received PY - 2010/05/10/revised PY - 2010/05/11/accepted PY - 2010/7/28/entrez PY - 2010/7/28/pubmed PY - 2010/11/3/medline SP - 745 EP - 55 JF - Chemistry & biology JO - Chem. Biol. VL - 17 IS - 7 N2 - We have developed the first red fluorescent protein, named rsTagRFP, which possesses reversibly photoswitchable absorbance spectra. Illumination with blue and yellow light switches rsTagRFP into a red fluorescent state (ON state) or nonfluorescent state (OFF state), respectively. The ON and OFF states exhibit absorbance maxima at 567 and 440 nm, respectively. Due to the photoswitchable absorbance, rsTagRFP can be used as an acceptor for a photochromic Förster resonance energy transfer (pcFRET). The photochromic acceptor facilitates determination of a protein-protein interaction by providing an internal control for FRET. Using pcFRET with EYFP as a donor, we observed an interaction between epidermal growth factor receptor and growth factor receptor-binding protein 2 in live cells by detecting the modulation of both the fluorescence intensity and lifetime of the EYFP donor upon the ON-OFF photoswitching of the rsTagRFP acceptor. SN - 1879-1301 UR - https://www.unboundmedicine.com/medline/citation/20659687/Red_fluorescent_protein_with_reversibly_photoswitchable_absorbance_for_photochromic_FRET_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1074-5521(10)00213-9 DB - PRIME DP - Unbound Medicine ER -