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Detection of SYT and EWS gene rearrangements by dual-color break-apart CISH in liquid-based cytology samples of synovial sarcoma and Ewing sarcoma/primitive neuroectodermal tumor.
Am J Clin Pathol. 2010 Aug; 134(2):323-31.AJ

Abstract

To improve cytologic diagnostic accuracy for translocation-associated sarcomas, we explored dual-color break-apart (dc) chromogenic in situ hybridization (CISH) on liquid-based cytology (LBC) samples of 2 prototypic sarcomas: synovial sarcoma (SS) and Ewing sarcoma/primitive neuroectodermal tumor (ES/PNET). LBC samples of 10 cases of SS and 9 cases of ES/PNET were subjected to dc-CISH using probes for the specifically rearranged genes in each tumor entity: SYT in SS and EWS in ES/PNET. Rearranged SYT was successfully detected in all SSs but not in any ES/PNETs. In contrast, EWS rearrangement was identified in all ES/PNETs but not in any SSs. These results were validated by dc-fluorescence in situ hybridization and reverse transcription-polymerase chain reaction. dc-CISH on LBC samples is a reliable modality to detect gene rearrangements in sarcomas. This system has a clear advantage over other methods, enabling simultaneous visualization of the genetic abnormality and well-preserved, nonoverlapping cytomorphologic features with clear background under bright-field microscope.

Authors+Show Affiliations

Dept. of Pathology, Teikyo University School of Medicine, Kaga, Itabashi-ku, Tokyo 173-8605, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20660338

Citation

Kumagai, Arisa, et al. "Detection of SYT and EWS Gene Rearrangements By Dual-color Break-apart CISH in Liquid-based Cytology Samples of Synovial Sarcoma and Ewing Sarcoma/primitive Neuroectodermal Tumor." American Journal of Clinical Pathology, vol. 134, no. 2, 2010, pp. 323-31.
Kumagai A, Motoi T, Tsuji K, et al. Detection of SYT and EWS gene rearrangements by dual-color break-apart CISH in liquid-based cytology samples of synovial sarcoma and Ewing sarcoma/primitive neuroectodermal tumor. Am J Clin Pathol. 2010;134(2):323-31.
Kumagai, A., Motoi, T., Tsuji, K., Imamura, T., & Fukusato, T. (2010). Detection of SYT and EWS gene rearrangements by dual-color break-apart CISH in liquid-based cytology samples of synovial sarcoma and Ewing sarcoma/primitive neuroectodermal tumor. American Journal of Clinical Pathology, 134(2), 323-31. https://doi.org/10.1309/AJCPTLSM15XKPDDU
Kumagai A, et al. Detection of SYT and EWS Gene Rearrangements By Dual-color Break-apart CISH in Liquid-based Cytology Samples of Synovial Sarcoma and Ewing Sarcoma/primitive Neuroectodermal Tumor. Am J Clin Pathol. 2010;134(2):323-31. PubMed PMID: 20660338.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of SYT and EWS gene rearrangements by dual-color break-apart CISH in liquid-based cytology samples of synovial sarcoma and Ewing sarcoma/primitive neuroectodermal tumor. AU - Kumagai,Arisa, AU - Motoi,Toru, AU - Tsuji,Kaori, AU - Imamura,Tetsuo, AU - Fukusato,Toshio, PY - 2010/7/28/entrez PY - 2010/7/28/pubmed PY - 2010/8/21/medline SP - 323 EP - 31 JF - American journal of clinical pathology JO - Am J Clin Pathol VL - 134 IS - 2 N2 - To improve cytologic diagnostic accuracy for translocation-associated sarcomas, we explored dual-color break-apart (dc) chromogenic in situ hybridization (CISH) on liquid-based cytology (LBC) samples of 2 prototypic sarcomas: synovial sarcoma (SS) and Ewing sarcoma/primitive neuroectodermal tumor (ES/PNET). LBC samples of 10 cases of SS and 9 cases of ES/PNET were subjected to dc-CISH using probes for the specifically rearranged genes in each tumor entity: SYT in SS and EWS in ES/PNET. Rearranged SYT was successfully detected in all SSs but not in any ES/PNETs. In contrast, EWS rearrangement was identified in all ES/PNETs but not in any SSs. These results were validated by dc-fluorescence in situ hybridization and reverse transcription-polymerase chain reaction. dc-CISH on LBC samples is a reliable modality to detect gene rearrangements in sarcomas. This system has a clear advantage over other methods, enabling simultaneous visualization of the genetic abnormality and well-preserved, nonoverlapping cytomorphologic features with clear background under bright-field microscope. SN - 1943-7722 UR - https://www.unboundmedicine.com/medline/citation/20660338/Detection_of_SYT_and_EWS_gene_rearrangements_by_dual_color_break_apart_CISH_in_liquid_based_cytology_samples_of_synovial_sarcoma_and_Ewing_sarcoma/primitive_neuroectodermal_tumor_ L2 - https://academic.oup.com/ajcp/article-lookup/doi/10.1309/AJCPTLSM15XKPDDU DB - PRIME DP - Unbound Medicine ER -