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Suppression of tumour necrosis factor-alpha by Schizonepeta tenuifolia water extract via inhibition of IkappaBalpha degradation and Jun N-terminal kinase/stress-activated protein kinase activation.
J Pharm Pharmacol. 2010 Aug; 62(8):1069-76.JP

Abstract

OBJECTIVES

The anti-inflammatory effects of an aqueous extract of Schizonepeta tenuifolia on lipopolysaccharide (LPS)-induced tumour necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) in vivo and in vitro have been investigated.

METHODS

C57BL/6 mice were orally administered phosphate-buffered saline (control) or S. tenuifolia water extract (50, 200, 500 or 1000 mg/kg) for 10 days before intraperitoneal administration of LPS (1.3 mg/kg). Blood samples were obtained 1 h after LPS challenge, followed by determination of TNF-alpha and IL-6 levels. Peritoneal macrophages from thioglycollate-injected mice were obtained and stimulated with LPS and S. tenuifolia water extract for viability assay, cytokine analysis, real-time RT PCR and Western blotting.

KEY FINDINGS

Oral administration of S. tenuifolia water extract to mice significantly reduced LPS-induced serum levels of TNF-alpha, but not IL-6. When peritoneal macrophages were treated in vitro with S. tenuifolia water extract, the inhibition of LPS-induced TNF-alpha was more pronounced than that of IL-6 at the level of secreted protein and mRNA. S. tenuifolia water extract reduced the degradation of IkappaBalpha and the nuclear relocation of p65 NF-kappaB, but the phosphorylation of IkappaBalpha was not affected. Inhibition of c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) by S. tenuifolia water extract led secondarily to the inhibition of phospho-c-Jun and phospho-ATF-2.

CONCLUSIONS

These results indicated that the downregulation of TNF-alpha by S. tenuifolia water extract may have involved the inhibition of both IkappaBalpha degradation and activation of c-Jun and ATF-2 involving suppression of JNK/SAPK.

Authors+Show Affiliations

Department of East-West Medical Science, Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si, Gyeonggi-do, South Korea. shehee@khu.ac.krNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20663042

Citation

Kang, Hee, et al. "Suppression of Tumour Necrosis Factor-alpha By Schizonepeta Tenuifolia Water Extract Via Inhibition of IkappaBalpha Degradation and Jun N-terminal Kinase/stress-activated Protein Kinase Activation." The Journal of Pharmacy and Pharmacology, vol. 62, no. 8, 2010, pp. 1069-76.
Kang H, Han SW, Hong JW, et al. Suppression of tumour necrosis factor-alpha by Schizonepeta tenuifolia water extract via inhibition of IkappaBalpha degradation and Jun N-terminal kinase/stress-activated protein kinase activation. J Pharm Pharmacol. 2010;62(8):1069-76.
Kang, H., Han, S. W., Hong, J. W., & Sohn, N. W. (2010). Suppression of tumour necrosis factor-alpha by Schizonepeta tenuifolia water extract via inhibition of IkappaBalpha degradation and Jun N-terminal kinase/stress-activated protein kinase activation. The Journal of Pharmacy and Pharmacology, 62(8), 1069-76. https://doi.org/10.1111/j.2042-7158.2010.01126.x
Kang H, et al. Suppression of Tumour Necrosis Factor-alpha By Schizonepeta Tenuifolia Water Extract Via Inhibition of IkappaBalpha Degradation and Jun N-terminal Kinase/stress-activated Protein Kinase Activation. J Pharm Pharmacol. 2010;62(8):1069-76. PubMed PMID: 20663042.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Suppression of tumour necrosis factor-alpha by Schizonepeta tenuifolia water extract via inhibition of IkappaBalpha degradation and Jun N-terminal kinase/stress-activated protein kinase activation. AU - Kang,Hee, AU - Han,Sang-Woo, AU - Hong,Joung-Woo, AU - Sohn,Nak-Won, PY - 2010/7/29/entrez PY - 2010/7/29/pubmed PY - 2010/11/16/medline SP - 1069 EP - 76 JF - The Journal of pharmacy and pharmacology JO - J Pharm Pharmacol VL - 62 IS - 8 N2 - OBJECTIVES: The anti-inflammatory effects of an aqueous extract of Schizonepeta tenuifolia on lipopolysaccharide (LPS)-induced tumour necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) in vivo and in vitro have been investigated. METHODS: C57BL/6 mice were orally administered phosphate-buffered saline (control) or S. tenuifolia water extract (50, 200, 500 or 1000 mg/kg) for 10 days before intraperitoneal administration of LPS (1.3 mg/kg). Blood samples were obtained 1 h after LPS challenge, followed by determination of TNF-alpha and IL-6 levels. Peritoneal macrophages from thioglycollate-injected mice were obtained and stimulated with LPS and S. tenuifolia water extract for viability assay, cytokine analysis, real-time RT PCR and Western blotting. KEY FINDINGS: Oral administration of S. tenuifolia water extract to mice significantly reduced LPS-induced serum levels of TNF-alpha, but not IL-6. When peritoneal macrophages were treated in vitro with S. tenuifolia water extract, the inhibition of LPS-induced TNF-alpha was more pronounced than that of IL-6 at the level of secreted protein and mRNA. S. tenuifolia water extract reduced the degradation of IkappaBalpha and the nuclear relocation of p65 NF-kappaB, but the phosphorylation of IkappaBalpha was not affected. Inhibition of c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) by S. tenuifolia water extract led secondarily to the inhibition of phospho-c-Jun and phospho-ATF-2. CONCLUSIONS: These results indicated that the downregulation of TNF-alpha by S. tenuifolia water extract may have involved the inhibition of both IkappaBalpha degradation and activation of c-Jun and ATF-2 involving suppression of JNK/SAPK. SN - 2042-7158 UR - https://www.unboundmedicine.com/medline/citation/20663042/Suppression_of_tumour_necrosis_factor_alpha_by_Schizonepeta_tenuifolia_water_extract_via_inhibition_of_IkappaBalpha_degradation_and_Jun_N_terminal_kinase/stress_activated_protein_kinase_activation_ L2 - https://doi.org/10.1111/j.2042-7158.2010.01126.x DB - PRIME DP - Unbound Medicine ER -