Tags

Type your tag names separated by a space and hit enter

Isolation of antioxidants from Psoralea corylifolia fruits using high-speed counter-current chromatography guided by thin layer chromatography-antioxidant autographic assay.
J Chromatogr A. 2010 Aug 20; 1217(34):5470-6.JC

Abstract

A combinative method using high-speed counter-current chromatography (HSCCC) and thin layer chromatography (TLC) as an antioxidant autographic assay was developed to separate antioxidant components from the fruits of Psoralea corylifolia. Under the guidance of TLC bioautography, eight compounds including five flavonoids and three coumarins were successfully separated from the fruits of P. corylifolia by HSCCC with an optimized two-phase solvent system, n-hexane-ethyl acetate-methanol-water (1:1.1:1.3:1, v/v/v/v). The separation produced 5.91mg psoralen, 6.26mg isopsoralen, 3.19mg psoralidin, 0.92mg corylifol A, and 2.43mg bavachinin with corresponding purities of 99.5, 99.8, 99.4, 96.4, and 99.0%, as well as three sub-fractions, in a single run from 250mg ethyl acetate fraction of P. corylifolia extract. Following an additional clean-up step by preparative TLC, 0.4mg 8-prenyldaidzein (purity 91.7%), 4.18mg neobavaisoflavone (purity 97.4%) and 4.36mg isobavachalcone (purity 96.8%) were separated from the three individual sub-fractions. The structures of the isolated compounds were identified by (1)H NMR and (13)C NMR. The results of antioxidant activity estimation by electron spin resonance (ESR) method showed that psoralidin was the most active antioxidant with an IC50 value of 44.7microM. This is the first report on simultaneous separation of eight compounds from P. corylifolia by HSCCC.

Links

Publisher Full Text

Authors+Show Affiliations

Xiao G
The MOE Key Laboratory for Standardization of Chinese Medicines, the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, and the Shanghai Key Laboratory for Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Zhangjiang Hi-Tech Park, Shanghai 201210, China.
Li G
No affiliation info available
Chen L
No affiliation info available
Zhang Z
No affiliation info available
Yin JJ
No affiliation info available
Wu T
No affiliation info available
Cheng Z
No affiliation info available
Wei X
No affiliation info available
Wang Z
No affiliation info available

MeSH

AcetatesAntioxidantsAutoradiographyBiphenyl CompoundsChromatography, Thin LayerCoumarinsCountercurrent DistributionEthanolFlavonoidsFruitNuclear Magnetic Resonance, BiomolecularPicratesPlant ExtractsPsoralea

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20663508

Citation

Xiao, Guodong, et al. "Isolation of Antioxidants From Psoralea Corylifolia Fruits Using High-speed Counter-current Chromatography Guided By Thin Layer Chromatography-antioxidant Autographic Assay." Journal of Chromatography. A, vol. 1217, no. 34, 2010, pp. 5470-6.
Xiao G, Li G, Chen L, et al. Isolation of antioxidants from Psoralea corylifolia fruits using high-speed counter-current chromatography guided by thin layer chromatography-antioxidant autographic assay. J Chromatogr A. 2010;1217(34):5470-6.
Xiao, G., Li, G., Chen, L., Zhang, Z., Yin, J. J., Wu, T., Cheng, Z., Wei, X., & Wang, Z. (2010). Isolation of antioxidants from Psoralea corylifolia fruits using high-speed counter-current chromatography guided by thin layer chromatography-antioxidant autographic assay. Journal of Chromatography. A, 1217(34), 5470-6. https://doi.org/10.1016/j.chroma.2010.06.041
Xiao G, et al. Isolation of Antioxidants From Psoralea Corylifolia Fruits Using High-speed Counter-current Chromatography Guided By Thin Layer Chromatography-antioxidant Autographic Assay. J Chromatogr A. 2010 Aug 20;1217(34):5470-6. PubMed PMID: 20663508.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation of antioxidants from Psoralea corylifolia fruits using high-speed counter-current chromatography guided by thin layer chromatography-antioxidant autographic assay. AU - Xiao,Guodong, AU - Li,Guowen, AU - Chen,Liang, AU - Zhang,Zijia, AU - Yin,Jun-Jie, AU - Wu,Tao, AU - Cheng,Zhihong, AU - Wei,Xiaohui, AU - Wang,Zhengtao, Y1 - 2010/06/23/ PY - 2009/12/30/received PY - 2010/06/12/revised PY - 2010/06/16/accepted PY - 2010/7/29/entrez PY - 2010/7/29/pubmed PY - 2010/11/16/medline SP - 5470 EP - 6 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1217 IS - 34 N2 - A combinative method using high-speed counter-current chromatography (HSCCC) and thin layer chromatography (TLC) as an antioxidant autographic assay was developed to separate antioxidant components from the fruits of Psoralea corylifolia. Under the guidance of TLC bioautography, eight compounds including five flavonoids and three coumarins were successfully separated from the fruits of P. corylifolia by HSCCC with an optimized two-phase solvent system, n-hexane-ethyl acetate-methanol-water (1:1.1:1.3:1, v/v/v/v). The separation produced 5.91mg psoralen, 6.26mg isopsoralen, 3.19mg psoralidin, 0.92mg corylifol A, and 2.43mg bavachinin with corresponding purities of 99.5, 99.8, 99.4, 96.4, and 99.0%, as well as three sub-fractions, in a single run from 250mg ethyl acetate fraction of P. corylifolia extract. Following an additional clean-up step by preparative TLC, 0.4mg 8-prenyldaidzein (purity 91.7%), 4.18mg neobavaisoflavone (purity 97.4%) and 4.36mg isobavachalcone (purity 96.8%) were separated from the three individual sub-fractions. The structures of the isolated compounds were identified by (1)H NMR and (13)C NMR. The results of antioxidant activity estimation by electron spin resonance (ESR) method showed that psoralidin was the most active antioxidant with an IC50 value of 44.7microM. This is the first report on simultaneous separation of eight compounds from P. corylifolia by HSCCC. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/20663508/Isolation_of_antioxidants_from_Psoralea_corylifolia_fruits_using_high_speed_counter_current_chromatography_guided_by_thin_layer_chromatography_antioxidant_autographic_assay_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(10)00828-9 DB - PRIME DP - Unbound Medicine ER -