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Oridonin induces G2/M cell cycle arrest and apoptosis through MAPK and p53 signaling pathways in HepG2 cells.
Oncol Rep. 2010 Sep; 24(3):647-51.OR

Abstract

Oridonin, the main active constituent of Isodon rubescen, has antihepatocarcinoma activity in experimental and clinical settings. The aims of the study were to explore the anticancer effect of oridonin in HepG2 cells and to investigate the underlying mechanisms. Results showed that oridonin treatment for 24 or 48 h resulted in a marked decrease in cell viability time- and dose-dependently. IC50 values were determined as 38.86 microM and 24.90 microM for 24-h and 48-h treatments, respectively. Flow cytometric analysis showed that a 24-h treatment of 40 microM oridonin induced G2/M cell cycle arrest and apoptosis. Typical apoptotic nucleus alterations were observed with fluorescence microscope after DAPI staining. Immunoblot analysis demonstrated that oridonin treatment increased expression levels of p-JNK, p-p38, p-p53 and p21, elevated the level of cyclin B1/p-Cdc2 (Tyr15) complex, and inhibited the expression of p-ERK. Moreover, oridonin treatment activated caspase-9 and caspase-3. In conclusion, oridonin induced G2/M cell cycle arrest and apoptosis in HepG2 cells through MAPK and p53 pathways, which advances our understanding on the molecular mechanisms of oridonin in hepatocarcinoma management.

Authors+Show Affiliations

Center for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20664969

Citation

Wang, Hui, et al. "Oridonin Induces G2/M Cell Cycle Arrest and Apoptosis Through MAPK and P53 Signaling Pathways in HepG2 Cells." Oncology Reports, vol. 24, no. 3, 2010, pp. 647-51.
Wang H, Ye Y, Chui JH, et al. Oridonin induces G2/M cell cycle arrest and apoptosis through MAPK and p53 signaling pathways in HepG2 cells. Oncol Rep. 2010;24(3):647-51.
Wang, H., Ye, Y., Chui, J. H., Zhu, G. Y., Li, Y. W., Fong, D. W., & Yu, Z. L. (2010). Oridonin induces G2/M cell cycle arrest and apoptosis through MAPK and p53 signaling pathways in HepG2 cells. Oncology Reports, 24(3), 647-51.
Wang H, et al. Oridonin Induces G2/M Cell Cycle Arrest and Apoptosis Through MAPK and P53 Signaling Pathways in HepG2 Cells. Oncol Rep. 2010;24(3):647-51. PubMed PMID: 20664969.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oridonin induces G2/M cell cycle arrest and apoptosis through MAPK and p53 signaling pathways in HepG2 cells. AU - Wang,Hui, AU - Ye,Yan, AU - Chui,Jian-Hong, AU - Zhu,Guo-Yuan, AU - Li,Ying-Wei, AU - Fong,David W F, AU - Yu,Zhi-Ling, PY - 2010/7/29/entrez PY - 2010/7/29/pubmed PY - 2010/12/14/medline SP - 647 EP - 51 JF - Oncology reports JO - Oncol Rep VL - 24 IS - 3 N2 - Oridonin, the main active constituent of Isodon rubescen, has antihepatocarcinoma activity in experimental and clinical settings. The aims of the study were to explore the anticancer effect of oridonin in HepG2 cells and to investigate the underlying mechanisms. Results showed that oridonin treatment for 24 or 48 h resulted in a marked decrease in cell viability time- and dose-dependently. IC50 values were determined as 38.86 microM and 24.90 microM for 24-h and 48-h treatments, respectively. Flow cytometric analysis showed that a 24-h treatment of 40 microM oridonin induced G2/M cell cycle arrest and apoptosis. Typical apoptotic nucleus alterations were observed with fluorescence microscope after DAPI staining. Immunoblot analysis demonstrated that oridonin treatment increased expression levels of p-JNK, p-p38, p-p53 and p21, elevated the level of cyclin B1/p-Cdc2 (Tyr15) complex, and inhibited the expression of p-ERK. Moreover, oridonin treatment activated caspase-9 and caspase-3. In conclusion, oridonin induced G2/M cell cycle arrest and apoptosis in HepG2 cells through MAPK and p53 pathways, which advances our understanding on the molecular mechanisms of oridonin in hepatocarcinoma management. SN - 1791-2431 UR - https://www.unboundmedicine.com/medline/citation/20664969/Oridonin_induces_G2/M_cell_cycle_arrest_and_apoptosis_through_MAPK_and_p53_signaling_pathways_in_HepG2_cells_ L2 - http://www.spandidos-publications.com/or/24/3/647 DB - PRIME DP - Unbound Medicine ER -