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Inorganic polyphosphates in mitochondria.
Biochemistry (Mosc). 2010 Jul; 75(7):825-31.B

Abstract

Current data concerning the crucial role of inorganic polyphosphates (polyP) in mitochondrial functions and dysfunctions in yeast and animal cells are reviewed. Biopolymers with short chain length (approximately 15 phosphate residues) were found in the mitochondria of Saccharomyces cerevisiae. They comprised 7-10% of the total polyP content of the cell. The polyP are located in the membranes and intermembrane space of mitochondria. The mitochondrial membranes possess polyP/Ca2+/polyhydroxybutyrate complexes. PolyP accumulation is typical of promitochondria but not of functionally active mitochondria. Yeast mitochondria possess two exopolyphosphatases splitting P(i) from the end of the polyP chain. One of them, encoded by the PPX1 gene, is located in the matrix; the other one, encoded by the PPN1 gene, is membrane-bound. Formation of well-developed mitochondria in the cells of S. cerevisiae after glucose depletion is accompanied by decrease in the polyP level and the chain length. In PPN1 mutants, the polyP chain length increased under glucose consumption, and the formation of well-developed mitochondria was blocked. These mutants were defective in respiration functions and consumption of oxidizable carbon sources such as lactate and ethanol. Since polyP is a compound with high-energy bonds, its metabolism vitally depends on the cell bioenergetics. The maximal level of short-chain acid-soluble polyP was observed in S. cerevisiae under consumption of glucose, while the long-chain polyP prevailed under ethanol consumption. In insects, polyP in the mitochondria change drastically during ontogenetic development, indicating involvement of the polymers in the regulation of mitochondrial metabolism during ontogenesis. In human cell lines, specific reduction of mitochondrial polyP under expression of yeast exopolyphosphatase PPX1 significantly modulates mitochondrial bioenergetics and transport.

Authors+Show Affiliations

Kulakovskaya TV
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, Russia. alla@ibpm.pushchino.ru
Lichko LP
No affiliation info available
Vagabov VM
No affiliation info available
Kulaev IS
No affiliation info available

MeSH

Acid Anhydride HydrolasesAnimalsCell LineHumansMitochondriaMolecular StructurePolyphosphatesSaccharomyces cerevisiaeSaccharomyces cerevisiae Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

20673205

Citation

Kulakovskaya, T V., et al. "Inorganic Polyphosphates in Mitochondria." Biochemistry. Biokhimiia, vol. 75, no. 7, 2010, pp. 825-31.
Kulakovskaya TV, Lichko LP, Vagabov VM, et al. Inorganic polyphosphates in mitochondria. Biochemistry (Mosc). 2010;75(7):825-31.
Kulakovskaya, T. V., Lichko, L. P., Vagabov, V. M., & Kulaev, I. S. (2010). Inorganic polyphosphates in mitochondria. Biochemistry. Biokhimiia, 75(7), 825-31.
Kulakovskaya TV, et al. Inorganic Polyphosphates in Mitochondria. Biochemistry (Mosc). 2010;75(7):825-31. PubMed PMID: 20673205.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inorganic polyphosphates in mitochondria. AU - Kulakovskaya,T V, AU - Lichko,L P, AU - Vagabov,V M, AU - Kulaev,I S, PY - 2010/8/3/entrez PY - 2010/8/3/pubmed PY - 2010/11/5/medline SP - 825 EP - 31 JF - Biochemistry. Biokhimiia JO - Biochemistry (Mosc) VL - 75 IS - 7 N2 - Current data concerning the crucial role of inorganic polyphosphates (polyP) in mitochondrial functions and dysfunctions in yeast and animal cells are reviewed. Biopolymers with short chain length (approximately 15 phosphate residues) were found in the mitochondria of Saccharomyces cerevisiae. They comprised 7-10% of the total polyP content of the cell. The polyP are located in the membranes and intermembrane space of mitochondria. The mitochondrial membranes possess polyP/Ca2+/polyhydroxybutyrate complexes. PolyP accumulation is typical of promitochondria but not of functionally active mitochondria. Yeast mitochondria possess two exopolyphosphatases splitting P(i) from the end of the polyP chain. One of them, encoded by the PPX1 gene, is located in the matrix; the other one, encoded by the PPN1 gene, is membrane-bound. Formation of well-developed mitochondria in the cells of S. cerevisiae after glucose depletion is accompanied by decrease in the polyP level and the chain length. In PPN1 mutants, the polyP chain length increased under glucose consumption, and the formation of well-developed mitochondria was blocked. These mutants were defective in respiration functions and consumption of oxidizable carbon sources such as lactate and ethanol. Since polyP is a compound with high-energy bonds, its metabolism vitally depends on the cell bioenergetics. The maximal level of short-chain acid-soluble polyP was observed in S. cerevisiae under consumption of glucose, while the long-chain polyP prevailed under ethanol consumption. In insects, polyP in the mitochondria change drastically during ontogenetic development, indicating involvement of the polymers in the regulation of mitochondrial metabolism during ontogenesis. In human cell lines, specific reduction of mitochondrial polyP under expression of yeast exopolyphosphatase PPX1 significantly modulates mitochondrial bioenergetics and transport. SN - 1608-3040 UR - https://www.unboundmedicine.com/medline/citation/20673205/Inorganic_polyphosphates_in_mitochondria_ L2 - https://doi.org/10.1134/s0006297910070035 DB - PRIME DP - Unbound Medicine ER -