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Molecularly-imprinted microspheres for selective extraction and determination of melamine in milk and feed using gas chromatography-mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Sep 01; 878(25):2333-8.JC

Abstract

Molecularly-imprinted polymers in the form of microspheres were synthesized using the dispersion polymerization protocol; cyromazine was used as dummy template, while methacrylic acid, ethylene glycol dimethacrylate and acetonitrile (MeCN) were used as functional monomer, cross-linker, and porogen, respectively. When compared with the non-imprinted polymer, the molecularly-imprinted polymers (MIPs) showed outstanding affinity toward melamine in MeCN with a maximum binding concentration (B(max)) of 53.20 nmol mg(-1) MIPs, imprinting effect of 4.6, and a dissociation constant (K(d)) of 90.45 microM. After optimization of the molecularly-imprinted solid-phase extraction conditions, a new method was developed to determine the melamine in milk and feed with gas chromatography-mass spectrometry. The performance of this method has been evaluated in the tainted milk and feed in terms of recovery, precision, linearity, the limit of detection (LOD) and limit of quantitation (LOQ). Recovery ranged in samples from 93.1 to 101.3% with intra-day and inter-day relative standard deviation values below 5.34%. The LOD and LOQ of melamine in milk and feed were 0.01 microg mL(-1) (microg g(-1)) and 0.05 microg mL(-1) (microg g(-1)), respectively.

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Authors+Show Affiliations

Li M
State Key Laboratory of Animal Nutrition, China Agricultural University, 2 Yuanmingyuan West Road, Beijing 100193, PR China.
Zhang L
No affiliation info available
Meng Z
No affiliation info available
Wang Z
No affiliation info available
Wu H
No affiliation info available

MeSH

AcetonitrilesAnimal FeedAnimalsEthylene GlycolFood AnalysisGas Chromatography-Mass SpectrometryMethacrylatesMicroscopy, Electron, ScanningMicrospheresMilkMolecular ImprintingReproducibility of ResultsSensitivity and SpecificitySolid Phase ExtractionSolventsTriazines

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20674520

Citation

Li, Mu, et al. "Molecularly-imprinted Microspheres for Selective Extraction and Determination of Melamine in Milk and Feed Using Gas Chromatography-mass Spectrometry." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 878, no. 25, 2010, pp. 2333-8.
Li M, Zhang L, Meng Z, et al. Molecularly-imprinted microspheres for selective extraction and determination of melamine in milk and feed using gas chromatography-mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2010;878(25):2333-8.
Li, M., Zhang, L., Meng, Z., Wang, Z., & Wu, H. (2010). Molecularly-imprinted microspheres for selective extraction and determination of melamine in milk and feed using gas chromatography-mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 878(25), 2333-8. https://doi.org/10.1016/j.jchromb.2010.07.003
Li M, et al. Molecularly-imprinted Microspheres for Selective Extraction and Determination of Melamine in Milk and Feed Using Gas Chromatography-mass Spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Sep 1;878(25):2333-8. PubMed PMID: 20674520.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecularly-imprinted microspheres for selective extraction and determination of melamine in milk and feed using gas chromatography-mass spectrometry. AU - Li,Mu, AU - Zhang,Liying, AU - Meng,Zihui, AU - Wang,Zongyi, AU - Wu,Hui, Y1 - 2010/07/12/ PY - 2010/04/26/received PY - 2010/07/04/revised PY - 2010/07/05/accepted PY - 2010/8/3/entrez PY - 2010/8/3/pubmed PY - 2010/12/16/medline SP - 2333 EP - 8 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 878 IS - 25 N2 - Molecularly-imprinted polymers in the form of microspheres were synthesized using the dispersion polymerization protocol; cyromazine was used as dummy template, while methacrylic acid, ethylene glycol dimethacrylate and acetonitrile (MeCN) were used as functional monomer, cross-linker, and porogen, respectively. When compared with the non-imprinted polymer, the molecularly-imprinted polymers (MIPs) showed outstanding affinity toward melamine in MeCN with a maximum binding concentration (B(max)) of 53.20 nmol mg(-1) MIPs, imprinting effect of 4.6, and a dissociation constant (K(d)) of 90.45 microM. After optimization of the molecularly-imprinted solid-phase extraction conditions, a new method was developed to determine the melamine in milk and feed with gas chromatography-mass spectrometry. The performance of this method has been evaluated in the tainted milk and feed in terms of recovery, precision, linearity, the limit of detection (LOD) and limit of quantitation (LOQ). Recovery ranged in samples from 93.1 to 101.3% with intra-day and inter-day relative standard deviation values below 5.34%. The LOD and LOQ of melamine in milk and feed were 0.01 microg mL(-1) (microg g(-1)) and 0.05 microg mL(-1) (microg g(-1)), respectively. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/20674520/Molecularly_imprinted_microspheres_for_selective_extraction_and_determination_of_melamine_in_milk_and_feed_using_gas_chromatography_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(10)00436-8 DB - PRIME DP - Unbound Medicine ER -