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Evaluation of the safety of the dietary antioxidant ergothioneine using the bacterial reverse mutation assay.
Toxicology 2010; 278(1):39-45T

Abstract

The dietary antioxidant L-(+)-ergothioneine was tested for its potential mutagenic activity using the bacterial reverse mutation assay. The experiments were carried out using histidine-requiring auxotrophic strains of Salmonella typhimurium (Salmonella typhimurium TA98, TA100, TA1535 and TA1537), and the tryptophan-requiring auxotrophic strain of Escherichia coli (Escherichia coli WP2 uvrA) in the presence and absence of a post-mitochondrial supernatant (S9) prepared from livers of phenobarbital/β-naphthoflavone-induced rats. The revertant colony numbers of vehicle control plates with and without S9 Mix were within the corresponding historical control data ranges. The reference mutagen treatments (positive controls) showed the expected, biologically relevant increases in induced revertant colonies in all experimental phases in all tester strains. No biologically relevant increases were observed in revertant colony numbers of any of the five test strains following treatment with L-(+)-ergothioneine at any concentration level, either in the presence or absence of metabolic activation (S9 Mix) in the performed experiments. On the basis of the data reported, it can be concluded that L-(+)-ergothioneine did not induce gene mutations by base pair changes or frameshifts in the genome of the strains used. Thus L-(+)-ergothioneine has no mutagenic activity on the applied bacteria tester strains under the test conditions used in this study. Research is continuing to define the role of L-(+)-ergothioneine in disease pathophysiology. Further studies on its safety are suggested.

Authors+Show Affiliations

AIBMR Life Sciences, Inc., Natural and Medicinal Products Research, 4117 S. Meridian, Puyallup, WA 98373, USA. alex@aibmr.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20674647

Citation

Schauss, Alexander G., et al. "Evaluation of the Safety of the Dietary Antioxidant Ergothioneine Using the Bacterial Reverse Mutation Assay." Toxicology, vol. 278, no. 1, 2010, pp. 39-45.
Schauss AG, Vértesi A, Endres JR, et al. Evaluation of the safety of the dietary antioxidant ergothioneine using the bacterial reverse mutation assay. Toxicology. 2010;278(1):39-45.
Schauss, A. G., Vértesi, A., Endres, J. R., Hirka, G., Clewell, A., Qureshi, I., & Pasics, I. (2010). Evaluation of the safety of the dietary antioxidant ergothioneine using the bacterial reverse mutation assay. Toxicology, 278(1), pp. 39-45. doi:10.1016/j.tox.2010.07.015.
Schauss AG, et al. Evaluation of the Safety of the Dietary Antioxidant Ergothioneine Using the Bacterial Reverse Mutation Assay. Toxicology. 2010 Nov 28;278(1):39-45. PubMed PMID: 20674647.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the safety of the dietary antioxidant ergothioneine using the bacterial reverse mutation assay. AU - Schauss,Alexander G, AU - Vértesi,Adél, AU - Endres,John R, AU - Hirka,Gábor, AU - Clewell,Amy, AU - Qureshi,Irfan, AU - Pasics,Ilona, Y1 - 2010/07/30/ PY - 2010/07/20/received PY - 2010/07/22/accepted PY - 2010/8/3/entrez PY - 2010/8/3/pubmed PY - 2011/1/11/medline SP - 39 EP - 45 JF - Toxicology JO - Toxicology VL - 278 IS - 1 N2 - The dietary antioxidant L-(+)-ergothioneine was tested for its potential mutagenic activity using the bacterial reverse mutation assay. The experiments were carried out using histidine-requiring auxotrophic strains of Salmonella typhimurium (Salmonella typhimurium TA98, TA100, TA1535 and TA1537), and the tryptophan-requiring auxotrophic strain of Escherichia coli (Escherichia coli WP2 uvrA) in the presence and absence of a post-mitochondrial supernatant (S9) prepared from livers of phenobarbital/β-naphthoflavone-induced rats. The revertant colony numbers of vehicle control plates with and without S9 Mix were within the corresponding historical control data ranges. The reference mutagen treatments (positive controls) showed the expected, biologically relevant increases in induced revertant colonies in all experimental phases in all tester strains. No biologically relevant increases were observed in revertant colony numbers of any of the five test strains following treatment with L-(+)-ergothioneine at any concentration level, either in the presence or absence of metabolic activation (S9 Mix) in the performed experiments. On the basis of the data reported, it can be concluded that L-(+)-ergothioneine did not induce gene mutations by base pair changes or frameshifts in the genome of the strains used. Thus L-(+)-ergothioneine has no mutagenic activity on the applied bacteria tester strains under the test conditions used in this study. Research is continuing to define the role of L-(+)-ergothioneine in disease pathophysiology. Further studies on its safety are suggested. SN - 1879-3185 UR - https://www.unboundmedicine.com/medline/citation/20674647/Evaluation_of_the_safety_of_the_dietary_antioxidant_ergothioneine_using_the_bacterial_reverse_mutation_assay_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0300-483X(10)00294-5 DB - PRIME DP - Unbound Medicine ER -