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Direct ethanol production from cellulosic materials at high temperature using the thermotolerant yeast Kluyveromyces marxianus displaying cellulolytic enzymes.
Appl Microbiol Biotechnol. 2010 Sep; 88(1):381-8.AM

Abstract

To exploit cellulosic materials for fuel ethanol production, a microorganism capable of high temperature and simultaneous saccharification-fermentation has been required. However, a major drawback is the optimum temperature for the saccharification and fermentation. Most ethanol-fermenting microbes have an optimum temperature for ethanol fermentation ranging between 28 degrees C and 37 degrees C, while the activity of cellulolytic enzymes is highest at around 50 degrees C and significantly decreases with a decrease in temperature. Therefore, in the present study, a thermotolerant yeast, Kluyveromyces marxianus, which has high growth and fermentation at elevated temperatures, was used as a producer of ethanol from cellulose. The strain was genetically engineered to display Trichoderma reesei endoglucanase and Aspergillus aculeatus beta-glucosidase on the cell surface, which successfully converts a cellulosic beta-glucan to ethanol directly at 48 degrees C with a yield of 4.24 g/l from 10 g/l within 12 h. The yield (in grams of ethanol produced per gram of beta-glucan consumed) was 0.47 g/g, which corresponds to 92.2% of the theoretical yield. This indicates that high-temperature cellulose fermentation to ethanol can be efficiently accomplished using a recombinant K. marxianus strain displaying thermostable cellulolytic enzymes on the cell surface.

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Authors+Show Affiliations

Yanase S
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada, Kobe, 657-8501, Japan.
Hasunuma T
No affiliation info available
Yamada R
No affiliation info available
Tanaka T
No affiliation info available
Ogino C
No affiliation info available
Fukuda H
No affiliation info available
Kondo A
No affiliation info available

MeSH

AspergillusCellobioseCellulasesCelluloseEthanolFungal ProteinsGlucoseHot TemperatureKluyveromycesRecombinant ProteinsTrichoderma

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20676628

Citation

Yanase, Shuhei, et al. "Direct Ethanol Production From Cellulosic Materials at High Temperature Using the Thermotolerant Yeast Kluyveromyces Marxianus Displaying Cellulolytic Enzymes." Applied Microbiology and Biotechnology, vol. 88, no. 1, 2010, pp. 381-8.
Yanase S, Hasunuma T, Yamada R, et al. Direct ethanol production from cellulosic materials at high temperature using the thermotolerant yeast Kluyveromyces marxianus displaying cellulolytic enzymes. Appl Microbiol Biotechnol. 2010;88(1):381-8.
Yanase, S., Hasunuma, T., Yamada, R., Tanaka, T., Ogino, C., Fukuda, H., & Kondo, A. (2010). Direct ethanol production from cellulosic materials at high temperature using the thermotolerant yeast Kluyveromyces marxianus displaying cellulolytic enzymes. Applied Microbiology and Biotechnology, 88(1), 381-8. https://doi.org/10.1007/s00253-010-2784-z
Yanase S, et al. Direct Ethanol Production From Cellulosic Materials at High Temperature Using the Thermotolerant Yeast Kluyveromyces Marxianus Displaying Cellulolytic Enzymes. Appl Microbiol Biotechnol. 2010;88(1):381-8. PubMed PMID: 20676628.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct ethanol production from cellulosic materials at high temperature using the thermotolerant yeast Kluyveromyces marxianus displaying cellulolytic enzymes. AU - Yanase,Shuhei, AU - Hasunuma,Tomohisa, AU - Yamada,Ryosuke, AU - Tanaka,Tsutomu, AU - Ogino,Chiaki, AU - Fukuda,Hideki, AU - Kondo,Akihiko, Y1 - 2010/07/31/ PY - 2010/05/26/received PY - 2010/07/16/accepted PY - 2010/07/14/revised PY - 2010/8/3/entrez PY - 2010/8/3/pubmed PY - 2010/12/14/medline SP - 381 EP - 8 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 88 IS - 1 N2 - To exploit cellulosic materials for fuel ethanol production, a microorganism capable of high temperature and simultaneous saccharification-fermentation has been required. However, a major drawback is the optimum temperature for the saccharification and fermentation. Most ethanol-fermenting microbes have an optimum temperature for ethanol fermentation ranging between 28 degrees C and 37 degrees C, while the activity of cellulolytic enzymes is highest at around 50 degrees C and significantly decreases with a decrease in temperature. Therefore, in the present study, a thermotolerant yeast, Kluyveromyces marxianus, which has high growth and fermentation at elevated temperatures, was used as a producer of ethanol from cellulose. The strain was genetically engineered to display Trichoderma reesei endoglucanase and Aspergillus aculeatus beta-glucosidase on the cell surface, which successfully converts a cellulosic beta-glucan to ethanol directly at 48 degrees C with a yield of 4.24 g/l from 10 g/l within 12 h. The yield (in grams of ethanol produced per gram of beta-glucan consumed) was 0.47 g/g, which corresponds to 92.2% of the theoretical yield. This indicates that high-temperature cellulose fermentation to ethanol can be efficiently accomplished using a recombinant K. marxianus strain displaying thermostable cellulolytic enzymes on the cell surface. SN - 1432-0614 UR - https://www.unboundmedicine.com/medline/citation/20676628/Direct_ethanol_production_from_cellulosic_materials_at_high_temperature_using_the_thermotolerant_yeast_Kluyveromyces_marxianus_displaying_cellulolytic_enzymes_ L2 - https://dx.doi.org/10.1007/s00253-010-2784-z DB - PRIME DP - Unbound Medicine ER -