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Detection, molecular characterization, and clonal diversity of methicillin-resistant Staphylococcus aureus CC398 and CC97 in Spanish slaughter pigs of different age groups.
Foodborne Pathog Dis. 2010 Oct; 7(10):1269-77.FP

Abstract

The objective of this study was to determine the frequency of nasal carriage of methicillin-resistant Staphylococcus aureus (MRSA) in slaughter pigs, to characterize the recovered isolates, and to investigate their genomic relatedness. Nasal swabs were collected from 53 finishing-pigs (F-pigs) and 53 suckling-piglets (S-piglets) at two different abattoirs in La Rioja (Northern Spain) coming from six production holdings. MRSA isolates were characterized by spa−, agr−, SCCmec−, and multilocus sequence typing, pulsed-field gel electrophoresis (PFGE)-ApaI, toxin gene profiling, antimicrobial susceptibility, and determination of antimicrobial resistance genes. MRSA isolates were recovered from 11 F-pigs (14 isolates) and 26 S-piglets (30 isolates). Forty of the 44MRSA presented the spa-types t011, t108, t1197, and t2346, which corresponded to the sequence type ST398 and to the clonal complex CC398. Interestingly, the remaining four isolates from F-pigs presented the spa-type t3992, and they were ascribed to a new sequence type named ST1379 (a single-locus variant of ST97), which was included in clonal complex CC97. Five PFGE-ApaI clusters with up to nine individual patterns detected among our MRSA and low genomic relatedness was observed between F-pig and S-piglet isolates. All MRSA were positive for hla, hld, and hlg hemolysin genes. ST1379 isolates harbored eta, lukE/D, and hlg-2 toxin genes, whereas ST398 isolates were positive for hlb. A great variety of distinct resistance gene patterns were observed, most of them coming from F-pig isolates. MRSA virulence properties seem to be dependent of the isolate clonal lineage. This study showed that slaughter pigs are frequently colonized by MRSA CC398; moreover, the detection of strains belonging to CC97 underlines that other lineages are also able to spread in livestock. Further studies should assess the risk of CC398 and non-CC398 MRSA to enter the food chain as well as the human health implications.

Authors+Show Affiliations

Departamento de Agricultura y Alimentación, Universidad de La Rioja, Madre de Dios 51, Logroño, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20677918

Citation

Gómez-Sanz, Elena, et al. "Detection, Molecular Characterization, and Clonal Diversity of Methicillin-resistant Staphylococcus Aureus CC398 and CC97 in Spanish Slaughter Pigs of Different Age Groups." Foodborne Pathogens and Disease, vol. 7, no. 10, 2010, pp. 1269-77.
Gómez-Sanz E, Torres C, Lozano C, et al. Detection, molecular characterization, and clonal diversity of methicillin-resistant Staphylococcus aureus CC398 and CC97 in Spanish slaughter pigs of different age groups. Foodborne Pathog Dis. 2010;7(10):1269-77.
Gómez-Sanz, E., Torres, C., Lozano, C., Fernández-Pérez, R., Aspiroz, C., Ruiz-Larrea, F., & Zarazaga, M. (2010). Detection, molecular characterization, and clonal diversity of methicillin-resistant Staphylococcus aureus CC398 and CC97 in Spanish slaughter pigs of different age groups. Foodborne Pathogens and Disease, 7(10), 1269-77. https://doi.org/10.1089/fpd.2010.0610
Gómez-Sanz E, et al. Detection, Molecular Characterization, and Clonal Diversity of Methicillin-resistant Staphylococcus Aureus CC398 and CC97 in Spanish Slaughter Pigs of Different Age Groups. Foodborne Pathog Dis. 2010;7(10):1269-77. PubMed PMID: 20677918.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection, molecular characterization, and clonal diversity of methicillin-resistant Staphylococcus aureus CC398 and CC97 in Spanish slaughter pigs of different age groups. AU - Gómez-Sanz,Elena, AU - Torres,Carmen, AU - Lozano,Carmen, AU - Fernández-Pérez,Rocío, AU - Aspiroz,Carmen, AU - Ruiz-Larrea,Fernanda, AU - Zarazaga,Myriam, PY - 2010/8/4/entrez PY - 2010/8/4/pubmed PY - 2010/11/17/medline SP - 1269 EP - 77 JF - Foodborne pathogens and disease JO - Foodborne Pathog Dis VL - 7 IS - 10 N2 - The objective of this study was to determine the frequency of nasal carriage of methicillin-resistant Staphylococcus aureus (MRSA) in slaughter pigs, to characterize the recovered isolates, and to investigate their genomic relatedness. Nasal swabs were collected from 53 finishing-pigs (F-pigs) and 53 suckling-piglets (S-piglets) at two different abattoirs in La Rioja (Northern Spain) coming from six production holdings. MRSA isolates were characterized by spa−, agr−, SCCmec−, and multilocus sequence typing, pulsed-field gel electrophoresis (PFGE)-ApaI, toxin gene profiling, antimicrobial susceptibility, and determination of antimicrobial resistance genes. MRSA isolates were recovered from 11 F-pigs (14 isolates) and 26 S-piglets (30 isolates). Forty of the 44MRSA presented the spa-types t011, t108, t1197, and t2346, which corresponded to the sequence type ST398 and to the clonal complex CC398. Interestingly, the remaining four isolates from F-pigs presented the spa-type t3992, and they were ascribed to a new sequence type named ST1379 (a single-locus variant of ST97), which was included in clonal complex CC97. Five PFGE-ApaI clusters with up to nine individual patterns detected among our MRSA and low genomic relatedness was observed between F-pig and S-piglet isolates. All MRSA were positive for hla, hld, and hlg hemolysin genes. ST1379 isolates harbored eta, lukE/D, and hlg-2 toxin genes, whereas ST398 isolates were positive for hlb. A great variety of distinct resistance gene patterns were observed, most of them coming from F-pig isolates. MRSA virulence properties seem to be dependent of the isolate clonal lineage. This study showed that slaughter pigs are frequently colonized by MRSA CC398; moreover, the detection of strains belonging to CC97 underlines that other lineages are also able to spread in livestock. Further studies should assess the risk of CC398 and non-CC398 MRSA to enter the food chain as well as the human health implications. SN - 1556-7125 UR - https://www.unboundmedicine.com/medline/citation/20677918/Detection_molecular_characterization_and_clonal_diversity_of_methicillin_resistant_Staphylococcus_aureus_CC398_and_CC97_in_Spanish_slaughter_pigs_of_different_age_groups_ L2 - https://www.liebertpub.com/doi/10.1089/fpd.2010.0610?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -