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Urate is a ligand for the transcriptional regulator PecS.
J Mol Biol. 2010 Sep 24; 402(3):539-51.JM

Abstract

PecS is a member of the MarR (multiple antibiotic resistance regulator) family, which has been shown in Erwinia to regulate the expression of virulence genes. MarR homologs typically bind a small molecule ligand, resulting in attenuated DNA binding. For PecS, the natural ligand has not been identified. We have previously shown that urate is a ligand for the Deinococcus radiodurans-encoded MarR homolog HucR (hypothetical uricase regulator) and identified residues responsible for ligand binding. We show here that all four residues involved in urate binding and propagation of conformational changes to DNA recognition helices are conserved in PecS homologs, suggesting that urate is the ligand for PecS. Consistent with this prediction, Agrobacterium tumefaciens PecS specifically binds urate, and urate attenuates DNA binding in vitro. PecS binds two operator sites in the intergenic region between the divergent pecS gene and pecM genes, one of which features two partially overlapping repeats to which PecS binds as a dimer on opposite faces of the duplex. Notably, urate dissociates PecS from cognate DNA, allowing transcription of both genes in vivo. Taken together, our data show that urate is a ligand for PecS and suggest that urate serves a novel function in signaling the colonization of a host plant.

Authors+Show Affiliations

Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

20678501

Citation

Perera, Inoka C., and Anne Grove. "Urate Is a Ligand for the Transcriptional Regulator PecS." Journal of Molecular Biology, vol. 402, no. 3, 2010, pp. 539-51.
Perera IC, Grove A. Urate is a ligand for the transcriptional regulator PecS. J Mol Biol. 2010;402(3):539-51.
Perera, I. C., & Grove, A. (2010). Urate is a ligand for the transcriptional regulator PecS. Journal of Molecular Biology, 402(3), 539-51. https://doi.org/10.1016/j.jmb.2010.07.053
Perera IC, Grove A. Urate Is a Ligand for the Transcriptional Regulator PecS. J Mol Biol. 2010 Sep 24;402(3):539-51. PubMed PMID: 20678501.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Urate is a ligand for the transcriptional regulator PecS. AU - Perera,Inoka C, AU - Grove,Anne, Y1 - 2010/08/03/ PY - 2010/07/07/received PY - 2010/07/17/revised PY - 2010/07/26/accepted PY - 2010/8/4/entrez PY - 2010/8/4/pubmed PY - 2010/10/1/medline SP - 539 EP - 51 JF - Journal of molecular biology JO - J Mol Biol VL - 402 IS - 3 N2 - PecS is a member of the MarR (multiple antibiotic resistance regulator) family, which has been shown in Erwinia to regulate the expression of virulence genes. MarR homologs typically bind a small molecule ligand, resulting in attenuated DNA binding. For PecS, the natural ligand has not been identified. We have previously shown that urate is a ligand for the Deinococcus radiodurans-encoded MarR homolog HucR (hypothetical uricase regulator) and identified residues responsible for ligand binding. We show here that all four residues involved in urate binding and propagation of conformational changes to DNA recognition helices are conserved in PecS homologs, suggesting that urate is the ligand for PecS. Consistent with this prediction, Agrobacterium tumefaciens PecS specifically binds urate, and urate attenuates DNA binding in vitro. PecS binds two operator sites in the intergenic region between the divergent pecS gene and pecM genes, one of which features two partially overlapping repeats to which PecS binds as a dimer on opposite faces of the duplex. Notably, urate dissociates PecS from cognate DNA, allowing transcription of both genes in vivo. Taken together, our data show that urate is a ligand for PecS and suggest that urate serves a novel function in signaling the colonization of a host plant. SN - 1089-8638 UR - https://www.unboundmedicine.com/medline/citation/20678501/Urate_is_a_ligand_for_the_transcriptional_regulator_PecS_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2836(10)00829-6 DB - PRIME DP - Unbound Medicine ER -